Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment

Abstract Spinal cord injury results in irreversible tissue damage followed by a very limited recovery of function. In this study we investigated whether transplantation of undifferentiated human induced pluripotent stem cells (hiPSCs) into the injured rat spinal cord is able to induce morphological...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tamás Bellák, Zoltán Fekécs, Dénes Török, Zsuzsanna Táncos, Csilla Nemes, Zsófia Tézsla, László Gál, Suchitra Polgári, Julianna Kobolák, András Dinnyés, Antal Nógrádi, Krisztián Pajer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eaae80ba082d49dab07c3395dc571997
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eaae80ba082d49dab07c3395dc571997
record_format	dspace
spelling	oai:doaj.org-article:eaae80ba082d49dab07c3395dc5719972021-12-02T13:46:37ZGrafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment10.1038/s41598-020-79846-22045-2322https://doaj.org/article/eaae80ba082d49dab07c3395dc5719972020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79846-2https://doaj.org/toc/2045-2322Abstract Spinal cord injury results in irreversible tissue damage followed by a very limited recovery of function. In this study we investigated whether transplantation of undifferentiated human induced pluripotent stem cells (hiPSCs) into the injured rat spinal cord is able to induce morphological and functional improvement. hiPSCs were grafted intraspinally or intravenously one week after a thoracic (T11) spinal cord contusion injury performed in Fischer 344 rats. Grafted animals showed significantly better functional recovery than the control rats which received only contusion injury. Morphologically, the contusion cavity was significantly smaller, and the amount of spared tissue was significantly greater in grafted animals than in controls. Retrograde tracing studies showed a statistically significant increase in the number of FB-labeled neurons in different segments of the spinal cord, the brainstem and the sensorimotor cortex. The extent of functional improvement was inversely related to the amount of chondroitin-sulphate around the cavity and the astrocytic and microglial reactions in the injured segment. The grafts produced GDNF, IL-10 and MIP1-alpha for at least one week. These data suggest that grafted undifferentiated hiPSCs are able to induce morphological and functional recovery after spinal cord contusion injury.Tamás BellákZoltán FekécsDénes TörökZsuzsanna TáncosCsilla NemesZsófia TézslaLászló GálSuchitra PolgáriJulianna KobolákAndrás DinnyésAntal NógrádiKrisztián PajerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-19 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tamás Bellák Zoltán Fekécs Dénes Török Zsuzsanna Táncos Csilla Nemes Zsófia Tézsla László Gál Suchitra Polgári Julianna Kobolák András Dinnyés Antal Nógrádi Krisztián Pajer Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment
description	Abstract Spinal cord injury results in irreversible tissue damage followed by a very limited recovery of function. In this study we investigated whether transplantation of undifferentiated human induced pluripotent stem cells (hiPSCs) into the injured rat spinal cord is able to induce morphological and functional improvement. hiPSCs were grafted intraspinally or intravenously one week after a thoracic (T11) spinal cord contusion injury performed in Fischer 344 rats. Grafted animals showed significantly better functional recovery than the control rats which received only contusion injury. Morphologically, the contusion cavity was significantly smaller, and the amount of spared tissue was significantly greater in grafted animals than in controls. Retrograde tracing studies showed a statistically significant increase in the number of FB-labeled neurons in different segments of the spinal cord, the brainstem and the sensorimotor cortex. The extent of functional improvement was inversely related to the amount of chondroitin-sulphate around the cavity and the astrocytic and microglial reactions in the injured segment. The grafts produced GDNF, IL-10 and MIP1-alpha for at least one week. These data suggest that grafted undifferentiated hiPSCs are able to induce morphological and functional recovery after spinal cord contusion injury.
format	article
author	Tamás Bellák Zoltán Fekécs Dénes Török Zsuzsanna Táncos Csilla Nemes Zsófia Tézsla László Gál Suchitra Polgári Julianna Kobolák András Dinnyés Antal Nógrádi Krisztián Pajer
author_facet	Tamás Bellák Zoltán Fekécs Dénes Török Zsuzsanna Táncos Csilla Nemes Zsófia Tézsla László Gál Suchitra Polgári Julianna Kobolák András Dinnyés Antal Nógrádi Krisztián Pajer
author_sort	Tamás Bellák
title	Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment
title_short	Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment
title_full	Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment
title_fullStr	Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment
title_full_unstemmed	Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment
title_sort	grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/eaae80ba082d49dab07c3395dc571997
work_keys_str_mv	AT tamasbellak graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT zoltanfekecs graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT denestorok graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT zsuzsannatancos graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT csillanemes graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT zsofiatezsla graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT laszlogal graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT suchitrapolgari graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT juliannakobolak graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT andrasdinnyes graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT antalnogradi graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment AT krisztianpajer graftedhumaninducedpluripotentstemcellsimprovetheoutcomeofspinalcordinjurymodulationofthelesionmicroenvironment
_version_	1718392532204781568

Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment

Ejemplares similares