Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial

Abstract In this study, we evaluated the effects of autologous serum collected after two types of exercise on the in vitro inflammatory profile and T cell phenotype of resting peripheral blood mononuclear cells (PBMCs) in obese men. Serum samples and PBMCs were obtained from eight obese men who perf...

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Autores principales:	Gilson P. Dorneles, Igor M. da Silva, Maeli Andressa Santos, Viviane R. Elsner, Simone G. Fonseca, Alessandra Peres, Pedro R. T. Romão
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eab54c12fa7c45f89e2c640c18190d39
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spelling	oai:doaj.org-article:eab54c12fa7c45f89e2c640c18190d392021-12-02T12:33:14ZImmunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial10.1038/s41598-020-78750-z2045-2322https://doaj.org/article/eab54c12fa7c45f89e2c640c18190d392020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78750-zhttps://doaj.org/toc/2045-2322Abstract In this study, we evaluated the effects of autologous serum collected after two types of exercise on the in vitro inflammatory profile and T cell phenotype of resting peripheral blood mononuclear cells (PBMCs) in obese men. Serum samples and PBMCs were obtained from eight obese men who performed two exercise bouts—high intensity interval exercise (HIIE) and exhaustive exercise session to voluntary fatigue—in a randomized cross-over trial. Pre-exercise PBMCs were incubated with 50% autologous serum (collected before and after each exercise bout) for 4 h. In vitro experiments revealed that post-HIIE serum reduced the histone H4 acetylation status and NF-κB content of PBMCs and suppressed the production of both TNF-α and IL-6 by PBMCs, while increasing IL-10 production. Post-exhaustive exercise serum induced histone H4 hyperacetylation and mitochondrial depolarization in lymphocytes and increased TNF-α production. In vitro post-HIIE serum incubation resulted in an increase in the frequencies of CD4 + CTLA-4 + and CD4 + CD25+ T cells expressing CD39 and CD73. Post-exhaustive exercise serum decreased the frequency of CD4 + CD25 + CD73+ T cells but increased CD4 + CD25-CD39 + T cell frequency. Both post-exercise serums increased the proportions of CD4 + PD-1 + and CD8 + PD-1+ T cells. Blood serum factors released during exercise altered the immune response and T cell phenotype. The type of exercise impacted the immunomodulatory activity of the post-exercise serum on PBMCs.Gilson P. DornelesIgor M. da SilvaMaeli Andressa SantosViviane R. ElsnerSimone G. FonsecaAlessandra PeresPedro R. T. RomãoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gilson P. Dorneles Igor M. da Silva Maeli Andressa Santos Viviane R. Elsner Simone G. Fonseca Alessandra Peres Pedro R. T. Romão Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial
description	Abstract In this study, we evaluated the effects of autologous serum collected after two types of exercise on the in vitro inflammatory profile and T cell phenotype of resting peripheral blood mononuclear cells (PBMCs) in obese men. Serum samples and PBMCs were obtained from eight obese men who performed two exercise bouts—high intensity interval exercise (HIIE) and exhaustive exercise session to voluntary fatigue—in a randomized cross-over trial. Pre-exercise PBMCs were incubated with 50% autologous serum (collected before and after each exercise bout) for 4 h. In vitro experiments revealed that post-HIIE serum reduced the histone H4 acetylation status and NF-κB content of PBMCs and suppressed the production of both TNF-α and IL-6 by PBMCs, while increasing IL-10 production. Post-exhaustive exercise serum induced histone H4 hyperacetylation and mitochondrial depolarization in lymphocytes and increased TNF-α production. In vitro post-HIIE serum incubation resulted in an increase in the frequencies of CD4 + CTLA-4 + and CD4 + CD25+ T cells expressing CD39 and CD73. Post-exhaustive exercise serum decreased the frequency of CD4 + CD25 + CD73+ T cells but increased CD4 + CD25-CD39 + T cell frequency. Both post-exercise serums increased the proportions of CD4 + PD-1 + and CD8 + PD-1+ T cells. Blood serum factors released during exercise altered the immune response and T cell phenotype. The type of exercise impacted the immunomodulatory activity of the post-exercise serum on PBMCs.
format	article
author	Gilson P. Dorneles Igor M. da Silva Maeli Andressa Santos Viviane R. Elsner Simone G. Fonseca Alessandra Peres Pedro R. T. Romão
author_facet	Gilson P. Dorneles Igor M. da Silva Maeli Andressa Santos Viviane R. Elsner Simone G. Fonseca Alessandra Peres Pedro R. T. Romão
author_sort	Gilson P. Dorneles
title	Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial
title_short	Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial
title_full	Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial
title_fullStr	Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial
title_full_unstemmed	Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial
title_sort	immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/eab54c12fa7c45f89e2c640c18190d39
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Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial

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