Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions.

Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate the gene expressio...

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Autores principales:	Carmen Naturil-Alfonso, María Dels Desamparats Saenz-de-Juano, David S Peñaranda, José S Vicente, Francisco Marco-Jiménez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eab62b80d2ea4c119d8717d09db20dde
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spelling	oai:doaj.org-article:eab62b80d2ea4c119d8717d09db20dde2021-11-18T08:05:24ZTranscriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions.1932-620310.1371/journal.pone.0051271https://doaj.org/article/eab62b80d2ea4c119d8717d09db20dde2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23251477/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate the gene expression profile of rabbit parthenote embryos cultured under in vivo conditions using microarray analysis. Transcriptomic profiles indicate 2541 differentially expressed genes between parthenotes and normal in vivo fertilised blastocysts, of which 76 genes were upregulated and 16 genes downregulated in in vivo cultured parthenote blastocyst, using 3 fold-changes as a cut-off. While differentially upregulated expressed genes are related to transport and protein metabolic process, downregulated expressed genes are related to DNA and RNA binding. Using microarray data, 6 imprinted genes were identified as conserved among rabbits, humans and mice: GRB10, ATP10A, ZNF215, NDN, IMPACT and SFMBT2. We also found that 26 putative genes have at least one member of that gene family imprinted in other species. These data strengthen the view that a large fraction of genes is differentially expressed between parthenogenetic and normal embryos cultured under the same conditions and offer a new approach to the identification of imprinted genes in rabbit.Carmen Naturil-AlfonsoMaría Dels Desamparats Saenz-de-JuanoDavid S PeñarandaJosé S VicenteFrancisco Marco-JiménezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51271 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carmen Naturil-Alfonso María Dels Desamparats Saenz-de-Juano David S Peñaranda José S Vicente Francisco Marco-Jiménez Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions.
description	Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate the gene expression profile of rabbit parthenote embryos cultured under in vivo conditions using microarray analysis. Transcriptomic profiles indicate 2541 differentially expressed genes between parthenotes and normal in vivo fertilised blastocysts, of which 76 genes were upregulated and 16 genes downregulated in in vivo cultured parthenote blastocyst, using 3 fold-changes as a cut-off. While differentially upregulated expressed genes are related to transport and protein metabolic process, downregulated expressed genes are related to DNA and RNA binding. Using microarray data, 6 imprinted genes were identified as conserved among rabbits, humans and mice: GRB10, ATP10A, ZNF215, NDN, IMPACT and SFMBT2. We also found that 26 putative genes have at least one member of that gene family imprinted in other species. These data strengthen the view that a large fraction of genes is differentially expressed between parthenogenetic and normal embryos cultured under the same conditions and offer a new approach to the identification of imprinted genes in rabbit.
format	article
author	Carmen Naturil-Alfonso María Dels Desamparats Saenz-de-Juano David S Peñaranda José S Vicente Francisco Marco-Jiménez
author_facet	Carmen Naturil-Alfonso María Dels Desamparats Saenz-de-Juano David S Peñaranda José S Vicente Francisco Marco-Jiménez
author_sort	Carmen Naturil-Alfonso
title	Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions.
title_short	Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions.
title_full	Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions.
title_fullStr	Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions.
title_full_unstemmed	Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions.
title_sort	transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/eab62b80d2ea4c119d8717d09db20dde
work_keys_str_mv	AT carmennaturilalfonso transcriptomeprofilingofrabbitparthenogeneticblastocystsdevelopedunderinvivoconditions AT mariadelsdesamparatssaenzdejuano transcriptomeprofilingofrabbitparthenogeneticblastocystsdevelopedunderinvivoconditions AT davidspenaranda transcriptomeprofilingofrabbitparthenogeneticblastocystsdevelopedunderinvivoconditions AT josesvicente transcriptomeprofilingofrabbitparthenogeneticblastocystsdevelopedunderinvivoconditions AT franciscomarcojimenez transcriptomeprofilingofrabbitparthenogeneticblastocystsdevelopedunderinvivoconditions
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Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions.

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