Detecting Efflux Pumps Genes in the Fluoroquinolones Resistant and Sensitive Strains of Escherichia coli in Patients with Urinary Tract Infection in Qom, Iran

Background and Objectives: Efflux pumps are among the main mechanisms for antibiotic resistance in Escherichia coli (E.coli) strains. The present study aimed to investigate the relationship between 5 efflux pump genes; acrA, acrB, emrA, emrB, and mdtk and fluoroquinolone resistance in E.coli isolate...

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Autores principales:	Ebrahim Eshaghi, Alireza Shahvaroughi farahani, Sara Mousaei, Mohammad Khalifeh-Gholi, Jafar Adnani Sadati, Roohollah Fateh
Formato:	article
Lenguaje:	FA
Publicado:	Qom University of Medical Sciences 2021
Materias:	escherichia coli efflux pump ciprofloxacin norfloxacin ofloxacin levofloxacin acra protein e coli acrb protein e coli Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eabe007e4c1d400ca831ad67d3e8852a
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spelling	oai:doaj.org-article:eabe007e4c1d400ca831ad67d3e8852a2021-12-05T11:40:11ZDetecting Efflux Pumps Genes in the Fluoroquinolones Resistant and Sensitive Strains of Escherichia coli in Patients with Urinary Tract Infection in Qom, Iran1735-77992008-1375https://doaj.org/article/eabe007e4c1d400ca831ad67d3e8852a2021-05-01T00:00:00Zhttp://journal.muq.ac.ir/article-1-3000-en.htmlhttps://doaj.org/toc/1735-7799https://doaj.org/toc/2008-1375Background and Objectives: Efflux pumps are among the main mechanisms for antibiotic resistance in Escherichia coli (E.coli) strains. The present study aimed to investigate the relationship between 5 efflux pump genes; acrA, acrB, emrA, emrB, and mdtk and fluoroquinolone resistance in E.coli isolated from patients with urinary tract infections. Methods: In this descriptive cross-sectional study, clinical samples were collected from several medical centers in Qom City, Iran, from June 2017 to September 2017. After the detection and primary identification of E.coli isolations using culture and biochemical tests, the antimicrobial susceptibility strains to the fluoroquinolones, such as Ciprofloxacin, Norfloxacin, Ofloxacin, and Levofloxacin were determined by disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Identifying the genes encoding efflux pumps was performed in sensitive and resistant strains by Polymerase Chain Reaction (PCR) technique. Results: Of the collected 170 clinical samples, 100 strains of E.coli were isolated and identified based on phenotypic characteristics. Evaluating antibiotic susceptibility of the strains demonstrated that 63(63%) isolates presented resistance to fluoroquinolones. PCR assay demonstrated that the prevalence of acr- AB, emrAB, and mdtk genes among fluoroquinolones resistant isolates were equal to 100%, 98%, and 94%, respectively. Furthermore, the frequency of the mentioned genes in fluoroquinolone-sensitive isolates was measured to be 34.2%, 27.02%, and 13.5%, respectively. Conclusion: The present research results revealed that the presence of acrAB, emrAB, and mdtk efflux pumps genes is potentially a factor in resistance to fluoroquinolone antibiotics. The presence of these genes in sensitive strains may indicate that the genes are not transcribed or translated. According to the high resistance of E.coli strains to fluoroquinolone antibiotics, it is recommended that antibiotic susceptibility testing be performed before starting treatment.Ebrahim EshaghiAlireza Shahvaroughi farahaniSara MousaeiMohammad Khalifeh-GholiJafar Adnani SadatiRoohollah FatehQom University of Medical Sciencesarticleescherichia coliefflux pumpciprofloxacinnorfloxacinofloxacinlevofloxacinacra protein e coliacrb protein e coliMedicine (General)R5-920FAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 15, Iss 3, Pp 210-221 (2021)
institution	DOAJ
collection	DOAJ
language	FA
topic	escherichia coli efflux pump ciprofloxacin norfloxacin ofloxacin levofloxacin acra protein e coli acrb protein e coli Medicine (General) R5-920
spellingShingle	escherichia coli efflux pump ciprofloxacin norfloxacin ofloxacin levofloxacin acra protein e coli acrb protein e coli Medicine (General) R5-920 Ebrahim Eshaghi Alireza Shahvaroughi farahani Sara Mousaei Mohammad Khalifeh-Gholi Jafar Adnani Sadati Roohollah Fateh Detecting Efflux Pumps Genes in the Fluoroquinolones Resistant and Sensitive Strains of Escherichia coli in Patients with Urinary Tract Infection in Qom, Iran
description	Background and Objectives: Efflux pumps are among the main mechanisms for antibiotic resistance in Escherichia coli (E.coli) strains. The present study aimed to investigate the relationship between 5 efflux pump genes; acrA, acrB, emrA, emrB, and mdtk and fluoroquinolone resistance in E.coli isolated from patients with urinary tract infections. Methods: In this descriptive cross-sectional study, clinical samples were collected from several medical centers in Qom City, Iran, from June 2017 to September 2017. After the detection and primary identification of E.coli isolations using culture and biochemical tests, the antimicrobial susceptibility strains to the fluoroquinolones, such as Ciprofloxacin, Norfloxacin, Ofloxacin, and Levofloxacin were determined by disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Identifying the genes encoding efflux pumps was performed in sensitive and resistant strains by Polymerase Chain Reaction (PCR) technique. Results: Of the collected 170 clinical samples, 100 strains of E.coli were isolated and identified based on phenotypic characteristics. Evaluating antibiotic susceptibility of the strains demonstrated that 63(63%) isolates presented resistance to fluoroquinolones. PCR assay demonstrated that the prevalence of acr- AB, emrAB, and mdtk genes among fluoroquinolones resistant isolates were equal to 100%, 98%, and 94%, respectively. Furthermore, the frequency of the mentioned genes in fluoroquinolone-sensitive isolates was measured to be 34.2%, 27.02%, and 13.5%, respectively. Conclusion: The present research results revealed that the presence of acrAB, emrAB, and mdtk efflux pumps genes is potentially a factor in resistance to fluoroquinolone antibiotics. The presence of these genes in sensitive strains may indicate that the genes are not transcribed or translated. According to the high resistance of E.coli strains to fluoroquinolone antibiotics, it is recommended that antibiotic susceptibility testing be performed before starting treatment.
format	article
author	Ebrahim Eshaghi Alireza Shahvaroughi farahani Sara Mousaei Mohammad Khalifeh-Gholi Jafar Adnani Sadati Roohollah Fateh
author_facet	Ebrahim Eshaghi Alireza Shahvaroughi farahani Sara Mousaei Mohammad Khalifeh-Gholi Jafar Adnani Sadati Roohollah Fateh
author_sort	Ebrahim Eshaghi
title	Detecting Efflux Pumps Genes in the Fluoroquinolones Resistant and Sensitive Strains of Escherichia coli in Patients with Urinary Tract Infection in Qom, Iran
title_short	Detecting Efflux Pumps Genes in the Fluoroquinolones Resistant and Sensitive Strains of Escherichia coli in Patients with Urinary Tract Infection in Qom, Iran
title_full	Detecting Efflux Pumps Genes in the Fluoroquinolones Resistant and Sensitive Strains of Escherichia coli in Patients with Urinary Tract Infection in Qom, Iran
title_fullStr	Detecting Efflux Pumps Genes in the Fluoroquinolones Resistant and Sensitive Strains of Escherichia coli in Patients with Urinary Tract Infection in Qom, Iran
title_full_unstemmed	Detecting Efflux Pumps Genes in the Fluoroquinolones Resistant and Sensitive Strains of Escherichia coli in Patients with Urinary Tract Infection in Qom, Iran
title_sort	detecting efflux pumps genes in the fluoroquinolones resistant and sensitive strains of escherichia coli in patients with urinary tract infection in qom, iran
publisher	Qom University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/eabe007e4c1d400ca831ad67d3e8852a
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Detecting Efflux Pumps Genes in the Fluoroquinolones Resistant and Sensitive Strains of Escherichia coli in Patients with Urinary Tract Infection in Qom, Iran

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