Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury

Abstract Cell-based therapy using mesenchymal stem cells (MSCs) is a novel treatment strategy for spinal cord injury (SCI). MSCs can be isolated from various tissues, and their characteristics vary based on the source. However, reports demonstrating the effect of transplanted rat cranial bone-derive...

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Autores principales:	Yuyo Maeda, Takashi Otsuka, Masaaki Takeda, Takahito Okazaki, Kiyoharu Shimizu, Masashi Kuwabara, Masahiro Hosogai, Louis Yuge, Takafumi Mitsuhara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eac1922342e049178fcc7113195f8dd6
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spelling	oai:doaj.org-article:eac1922342e049178fcc7113195f8dd62021-11-14T12:20:01ZTransplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury10.1038/s41598-021-01490-12045-2322https://doaj.org/article/eac1922342e049178fcc7113195f8dd62021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01490-1https://doaj.org/toc/2045-2322Abstract Cell-based therapy using mesenchymal stem cells (MSCs) is a novel treatment strategy for spinal cord injury (SCI). MSCs can be isolated from various tissues, and their characteristics vary based on the source. However, reports demonstrating the effect of transplanted rat cranial bone-derived MSCs (rcMSCs) on rat SCI models are lacking. In this study, we determined the effect of transplanting rcMSCs in rat SCI models. MSCs were established from collected bone marrow and cranial bones. SCI rats were established using the weight-drop method and transplanted intravenously with MSCs at 24 h post SCI. The recovery of motor function and hindlimb electrophysiology was evaluated 4 weeks post transplantation. Electrophysiological recovery was evaluated by recording the transcranial electrical stimulation motor-evoked potentials. Tissue repair after SCI was assessed by calculating the cavity ratio. The expression of genes involved in the inflammatory response and cell death in the spinal cord tissue was assessed by real-time polymerase chain reaction. The transplantation of rcMSCs improved motor function and electrophysiology recovery, and reduced cavity ratio. The expression of proinflammatory cytokines was suppressed in the spinal cord tissues of the rats that received rcMSCs. These results demonstrate the efficacy of rcMSCs as cell-based therapy for SCI.Yuyo MaedaTakashi OtsukaMasaaki TakedaTakahito OkazakiKiyoharu ShimizuMasashi KuwabaraMasahiro HosogaiLouis YugeTakafumi MitsuharaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuyo Maeda Takashi Otsuka Masaaki Takeda Takahito Okazaki Kiyoharu Shimizu Masashi Kuwabara Masahiro Hosogai Louis Yuge Takafumi Mitsuhara Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury
description	Abstract Cell-based therapy using mesenchymal stem cells (MSCs) is a novel treatment strategy for spinal cord injury (SCI). MSCs can be isolated from various tissues, and their characteristics vary based on the source. However, reports demonstrating the effect of transplanted rat cranial bone-derived MSCs (rcMSCs) on rat SCI models are lacking. In this study, we determined the effect of transplanting rcMSCs in rat SCI models. MSCs were established from collected bone marrow and cranial bones. SCI rats were established using the weight-drop method and transplanted intravenously with MSCs at 24 h post SCI. The recovery of motor function and hindlimb electrophysiology was evaluated 4 weeks post transplantation. Electrophysiological recovery was evaluated by recording the transcranial electrical stimulation motor-evoked potentials. Tissue repair after SCI was assessed by calculating the cavity ratio. The expression of genes involved in the inflammatory response and cell death in the spinal cord tissue was assessed by real-time polymerase chain reaction. The transplantation of rcMSCs improved motor function and electrophysiology recovery, and reduced cavity ratio. The expression of proinflammatory cytokines was suppressed in the spinal cord tissues of the rats that received rcMSCs. These results demonstrate the efficacy of rcMSCs as cell-based therapy for SCI.
format	article
author	Yuyo Maeda Takashi Otsuka Masaaki Takeda Takahito Okazaki Kiyoharu Shimizu Masashi Kuwabara Masahiro Hosogai Louis Yuge Takafumi Mitsuhara
author_facet	Yuyo Maeda Takashi Otsuka Masaaki Takeda Takahito Okazaki Kiyoharu Shimizu Masashi Kuwabara Masahiro Hosogai Louis Yuge Takafumi Mitsuhara
author_sort	Yuyo Maeda
title	Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury
title_short	Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury
title_full	Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury
title_fullStr	Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury
title_full_unstemmed	Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury
title_sort	transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eac1922342e049178fcc7113195f8dd6
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Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury

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