Rapid screening of complex DNA samples by single-molecule amplification and sequencing.

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram qu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiaqi Huang, Zongli Zheng, Anders F Andersson, Lars Engstrand, Weimin Ye
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eac2739637ef4a72a0cdcb799b0ebe40
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eac2739637ef4a72a0cdcb799b0ebe40
record_format	dspace
spelling	oai:doaj.org-article:eac2739637ef4a72a0cdcb799b0ebe402021-11-18T06:53:40ZRapid screening of complex DNA samples by single-molecule amplification and sequencing.1932-620310.1371/journal.pone.0019723https://doaj.org/article/eac2739637ef4a72a0cdcb799b0ebe402011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625543/?tool=EBIhttps://doaj.org/toc/1932-6203Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.Jiaqi HuangZongli ZhengAnders F AnderssonLars EngstrandWeimin YePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19723 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiaqi Huang Zongli Zheng Anders F Andersson Lars Engstrand Weimin Ye Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
description	Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.
format	article
author	Jiaqi Huang Zongli Zheng Anders F Andersson Lars Engstrand Weimin Ye
author_facet	Jiaqi Huang Zongli Zheng Anders F Andersson Lars Engstrand Weimin Ye
author_sort	Jiaqi Huang
title	Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
title_short	Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
title_full	Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
title_fullStr	Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
title_full_unstemmed	Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
title_sort	rapid screening of complex dna samples by single-molecule amplification and sequencing.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/eac2739637ef4a72a0cdcb799b0ebe40
work_keys_str_mv	AT jiaqihuang rapidscreeningofcomplexdnasamplesbysinglemoleculeamplificationandsequencing AT zonglizheng rapidscreeningofcomplexdnasamplesbysinglemoleculeamplificationandsequencing AT andersfandersson rapidscreeningofcomplexdnasamplesbysinglemoleculeamplificationandsequencing AT larsengstrand rapidscreeningofcomplexdnasamplesbysinglemoleculeamplificationandsequencing AT weiminye rapidscreeningofcomplexdnasamplesbysinglemoleculeamplificationandsequencing
_version_	1718424263844691968

Rapid screening of complex DNA samples by single-molecule amplification and sequencing.

Ejemplares similares