Processing of retinal signals in normal and HCN deficient mice.

This study investigates the role of two different HCN channel isoforms in the light response of the outer retina. Taking advantage of HCN-deficient mice models and of in vitro (patch-clamp) and in vivo (ERG) recordings of retinal activity we show that HCN1 and HCN2 channels are expressed at distinct...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Luca Della Santina, Ilaria Piano, Lorenzo Cangiano, Antonella Caputo, Andreas Ludwig, Luigi Cervetto, Claudia Gargini
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eac2d5601d724b3c98b18ed5a28213a8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eac2d5601d724b3c98b18ed5a28213a8
record_format	dspace
spelling	oai:doaj.org-article:eac2d5601d724b3c98b18ed5a28213a82021-11-18T07:29:58ZProcessing of retinal signals in normal and HCN deficient mice.1932-620310.1371/journal.pone.0029812https://doaj.org/article/eac2d5601d724b3c98b18ed5a28213a82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22279546/?tool=EBIhttps://doaj.org/toc/1932-6203This study investigates the role of two different HCN channel isoforms in the light response of the outer retina. Taking advantage of HCN-deficient mice models and of in vitro (patch-clamp) and in vivo (ERG) recordings of retinal activity we show that HCN1 and HCN2 channels are expressed at distinct retinal sites and serve different functions. Specifically, HCN1 operate mainly at the level of the photoreceptor inner segment from where, together with other voltage sensitive channels, they control the time course of the response to bright light. Conversely, HCN2 channels are mainly expressed on the dendrites of bipolar cells and affect the response to dim lights. Single cell recordings in HCN1⁻/⁻ mice or during a pharmacological blockade of I(h) show that, contrary to previous reports, I(kx) alone is able to generate the fast initial transient in the rod bright flash response. Here we demonstrate that the relative contribution of I(h) and I(kx) to the rods' temporal tuning depends on the membrane potential. This is the first instance in which the light response of normal and HCN1- or HCN2-deficient mice is analyzed in single cells in retinal slice preparations and in integrated full field ERG responses from intact animals. This comparison reveals a high degree of correlation between single cell current clamp data and ERG measurements. A novel picture emerges showing that the temporal profile of the visual response to dim and bright luminance changes is separately determined by the coordinated gating of distinct voltage dependent conductances in photoreceptors and bipolar cells.Luca Della SantinaIlaria PianoLorenzo CangianoAntonella CaputoAndreas LudwigLuigi CervettoClaudia GarginiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29812 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luca Della Santina Ilaria Piano Lorenzo Cangiano Antonella Caputo Andreas Ludwig Luigi Cervetto Claudia Gargini Processing of retinal signals in normal and HCN deficient mice.
description	This study investigates the role of two different HCN channel isoforms in the light response of the outer retina. Taking advantage of HCN-deficient mice models and of in vitro (patch-clamp) and in vivo (ERG) recordings of retinal activity we show that HCN1 and HCN2 channels are expressed at distinct retinal sites and serve different functions. Specifically, HCN1 operate mainly at the level of the photoreceptor inner segment from where, together with other voltage sensitive channels, they control the time course of the response to bright light. Conversely, HCN2 channels are mainly expressed on the dendrites of bipolar cells and affect the response to dim lights. Single cell recordings in HCN1⁻/⁻ mice or during a pharmacological blockade of I(h) show that, contrary to previous reports, I(kx) alone is able to generate the fast initial transient in the rod bright flash response. Here we demonstrate that the relative contribution of I(h) and I(kx) to the rods' temporal tuning depends on the membrane potential. This is the first instance in which the light response of normal and HCN1- or HCN2-deficient mice is analyzed in single cells in retinal slice preparations and in integrated full field ERG responses from intact animals. This comparison reveals a high degree of correlation between single cell current clamp data and ERG measurements. A novel picture emerges showing that the temporal profile of the visual response to dim and bright luminance changes is separately determined by the coordinated gating of distinct voltage dependent conductances in photoreceptors and bipolar cells.
format	article
author	Luca Della Santina Ilaria Piano Lorenzo Cangiano Antonella Caputo Andreas Ludwig Luigi Cervetto Claudia Gargini
author_facet	Luca Della Santina Ilaria Piano Lorenzo Cangiano Antonella Caputo Andreas Ludwig Luigi Cervetto Claudia Gargini
author_sort	Luca Della Santina
title	Processing of retinal signals in normal and HCN deficient mice.
title_short	Processing of retinal signals in normal and HCN deficient mice.
title_full	Processing of retinal signals in normal and HCN deficient mice.
title_fullStr	Processing of retinal signals in normal and HCN deficient mice.
title_full_unstemmed	Processing of retinal signals in normal and HCN deficient mice.
title_sort	processing of retinal signals in normal and hcn deficient mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/eac2d5601d724b3c98b18ed5a28213a8
work_keys_str_mv	AT lucadellasantina processingofretinalsignalsinnormalandhcndeficientmice AT ilariapiano processingofretinalsignalsinnormalandhcndeficientmice AT lorenzocangiano processingofretinalsignalsinnormalandhcndeficientmice AT antonellacaputo processingofretinalsignalsinnormalandhcndeficientmice AT andreasludwig processingofretinalsignalsinnormalandhcndeficientmice AT luigicervetto processingofretinalsignalsinnormalandhcndeficientmice AT claudiagargini processingofretinalsignalsinnormalandhcndeficientmice
_version_	1718423384724865024

Processing of retinal signals in normal and HCN deficient mice.

Ejemplares similares