Induction of antigen-specific immune tolerance using biodegradable nanoparticles containing antigen and dexamethasone

Sang-Hyun Kim, Jun-Hyeok Moon, Seong-Un Jeong, Ho-Hyun Jung, Chan-Su Park, Bang Yeon Hwang, Chong-Kil LeeCollege of Pharmacy, Chungbuk National University, Cheongju, KoreaPurpose: Dexamethasone (Dex) has long been used as a potent immunosuppressive agent in the treatment of inflammatory and autoimmu...

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Autores principales:	Kim SH, Moon JH, Jeong SU, Jung HH, Park CS, Hwang BY, Lee CK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Polymeric nanoparticle Dexamethasone Ovalbumin Tolerogenic dendritic cell Antigen-specific immune tolerance Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eac98eab5b3141de91a23faab0982f46
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spelling	oai:doaj.org-article:eac98eab5b3141de91a23faab0982f462021-12-02T03:06:29ZInduction of antigen-specific immune tolerance using biodegradable nanoparticles containing antigen and dexamethasone1178-2013https://doaj.org/article/eac98eab5b3141de91a23faab0982f462019-07-01T00:00:00Zhttps://www.dovepress.com/induction-of-antigen-specific-immune-tolerance-using-biodegradable-nan-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sang-Hyun Kim, Jun-Hyeok Moon, Seong-Un Jeong, Ho-Hyun Jung, Chan-Su Park, Bang Yeon Hwang, Chong-Kil LeeCollege of Pharmacy, Chungbuk National University, Cheongju, KoreaPurpose: Dexamethasone (Dex) has long been used as a potent immunosuppressive agent in the treatment of inflammatory and autoimmune diseases, despite serious side effects. In the present study, Dex and model antigen ovalbumin (OVA) were encapsulated with poly(lactic-co-glycolic acid) to deliver Dex and OVA preferentially to phagocytic cells, reducing systemic side effects of Dex. The OVA-specific immune tolerance-inducing activity of the nanoparticles (NPs) was examined.Methods: Polymeric NPs containing OVA and Dex (NP[OVA+Dex]) were prepared by the water-in-oil-in-water double emulsion solvent evaporation method. The effects of NP[OVA+Dex] on the maturation and function of immature dendritic cells (DCs) were examined in vitro. Furthermore, the OVA-specific immune tolerizing effects of NP[OVA+Dex] were confirmed in mice that were intravenously injected or orally fed with the NPs.Results: Immature DCs treated in vitro with NP[OVA+Dex] did not mature into immunogenic DCs but instead were converted into tolerogenic DCs. Furthermore, profoundly suppressed generation of OVA-specific cytotoxic T cells and production of OVA-specific IgG were observed in mice injected with NP[OVA+Dex], whereas regulatory T cells were concomitantly increased. Feeding of mice with NP[OVA+Dex] also induced OVA-specific immune tolerance.Conclusion: The present study demonstrates that oral feeding as well as intravenous injection of poly(lactic-co-glycolic acid) NPs encapsulating both antigen and Dex is a useful means of inducing antigen-specific immune tolerance, which is crucial for the treatment of autoimmune diseases.Keywords: polymeric nanoparticle, dexamethasone, ovalbumin, tolerogenic dendritic cell, antigen-specific immune toleranceKim SHMoon JHJeong SUJung HHPark CSHwang BYLee CKDove Medical PressarticlePolymeric nanoparticleDexamethasoneOvalbuminTolerogenic dendritic cellAntigen-specific immune toleranceMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 5229-5242 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Polymeric nanoparticle Dexamethasone Ovalbumin Tolerogenic dendritic cell Antigen-specific immune tolerance Medicine (General) R5-920
spellingShingle	Polymeric nanoparticle Dexamethasone Ovalbumin Tolerogenic dendritic cell Antigen-specific immune tolerance Medicine (General) R5-920 Kim SH Moon JH Jeong SU Jung HH Park CS Hwang BY Lee CK Induction of antigen-specific immune tolerance using biodegradable nanoparticles containing antigen and dexamethasone
description	Sang-Hyun Kim, Jun-Hyeok Moon, Seong-Un Jeong, Ho-Hyun Jung, Chan-Su Park, Bang Yeon Hwang, Chong-Kil LeeCollege of Pharmacy, Chungbuk National University, Cheongju, KoreaPurpose: Dexamethasone (Dex) has long been used as a potent immunosuppressive agent in the treatment of inflammatory and autoimmune diseases, despite serious side effects. In the present study, Dex and model antigen ovalbumin (OVA) were encapsulated with poly(lactic-co-glycolic acid) to deliver Dex and OVA preferentially to phagocytic cells, reducing systemic side effects of Dex. The OVA-specific immune tolerance-inducing activity of the nanoparticles (NPs) was examined.Methods: Polymeric NPs containing OVA and Dex (NP[OVA+Dex]) were prepared by the water-in-oil-in-water double emulsion solvent evaporation method. The effects of NP[OVA+Dex] on the maturation and function of immature dendritic cells (DCs) were examined in vitro. Furthermore, the OVA-specific immune tolerizing effects of NP[OVA+Dex] were confirmed in mice that were intravenously injected or orally fed with the NPs.Results: Immature DCs treated in vitro with NP[OVA+Dex] did not mature into immunogenic DCs but instead were converted into tolerogenic DCs. Furthermore, profoundly suppressed generation of OVA-specific cytotoxic T cells and production of OVA-specific IgG were observed in mice injected with NP[OVA+Dex], whereas regulatory T cells were concomitantly increased. Feeding of mice with NP[OVA+Dex] also induced OVA-specific immune tolerance.Conclusion: The present study demonstrates that oral feeding as well as intravenous injection of poly(lactic-co-glycolic acid) NPs encapsulating both antigen and Dex is a useful means of inducing antigen-specific immune tolerance, which is crucial for the treatment of autoimmune diseases.Keywords: polymeric nanoparticle, dexamethasone, ovalbumin, tolerogenic dendritic cell, antigen-specific immune tolerance
format	article
author	Kim SH Moon JH Jeong SU Jung HH Park CS Hwang BY Lee CK
author_facet	Kim SH Moon JH Jeong SU Jung HH Park CS Hwang BY Lee CK
author_sort	Kim SH
title	Induction of antigen-specific immune tolerance using biodegradable nanoparticles containing antigen and dexamethasone
title_short	Induction of antigen-specific immune tolerance using biodegradable nanoparticles containing antigen and dexamethasone
title_full	Induction of antigen-specific immune tolerance using biodegradable nanoparticles containing antigen and dexamethasone
title_fullStr	Induction of antigen-specific immune tolerance using biodegradable nanoparticles containing antigen and dexamethasone
title_full_unstemmed	Induction of antigen-specific immune tolerance using biodegradable nanoparticles containing antigen and dexamethasone
title_sort	induction of antigen-specific immune tolerance using biodegradable nanoparticles containing antigen and dexamethasone
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/eac98eab5b3141de91a23faab0982f46
work_keys_str_mv	AT kimsh inductionofantigenspecificimmunetoleranceusingbiodegradablenanoparticlescontainingantigenanddexamethasone AT moonjh inductionofantigenspecificimmunetoleranceusingbiodegradablenanoparticlescontainingantigenanddexamethasone AT jeongsu inductionofantigenspecificimmunetoleranceusingbiodegradablenanoparticlescontainingantigenanddexamethasone AT junghh inductionofantigenspecificimmunetoleranceusingbiodegradablenanoparticlescontainingantigenanddexamethasone AT parkcs inductionofantigenspecificimmunetoleranceusingbiodegradablenanoparticlescontainingantigenanddexamethasone AT hwangby inductionofantigenspecificimmunetoleranceusingbiodegradablenanoparticlescontainingantigenanddexamethasone AT leeck inductionofantigenspecificimmunetoleranceusingbiodegradablenanoparticlescontainingantigenanddexamethasone
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Induction of antigen-specific immune tolerance using biodegradable nanoparticles containing antigen and dexamethasone

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