Molecular Identification of Multiple Antibiotic Resistant Fish Pathogenic Enterococcus faecalis and their Control by Medicinal Herbs

Abstract The opportunistic fish pathogen, Enterococcus faecalis has been reported to cause mass mortality in several fish species in different countries. The objectives of this study were to (i) identify E. faecalis from the diseased fishes through molecular techniques; (ii) assess the antibiotic su...

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Autores principales: Muntasir Rahman, Md. Mahbubur Rahman, Suzan Chandra Deb, Md. Shahanoor Alam, Md. Jahangir Alam, Md. Tofazzal Islam
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Publicado: Nature Portfolio 2017
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spelling oai:doaj.org-article:eacfa5cf7946406390db51810d3ded962021-12-02T12:32:28ZMolecular Identification of Multiple Antibiotic Resistant Fish Pathogenic Enterococcus faecalis and their Control by Medicinal Herbs10.1038/s41598-017-03673-12045-2322https://doaj.org/article/eacfa5cf7946406390db51810d3ded962017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03673-1https://doaj.org/toc/2045-2322Abstract The opportunistic fish pathogen, Enterococcus faecalis has been reported to cause mass mortality in several fish species in different countries. The objectives of this study were to (i) identify E. faecalis from the diseased fishes through molecular techniques; (ii) assess the antibiotic susceptibility profile of E. faecalis isolates; and (iii) control disease in tilapia fish by treatment with medicinal plant extracts. A total of 48 isolates were phenotypically identified as Enterococcus species from tilapia, stinging catfish and walking catfish cultivated in several fish farms in Gazipur. Ten randomly selected isolates were identified as E. faecalis by 16S rRNA gene sequencing. Artificial infection revealed that most of the isolates caused moderate to high mortality in fishes with characteristic disease symptoms. These isolates exhibited resistance to multiple antibiotics in vitro. Bioassay revealed that organic extracts of Tamarindus indica and Emblica officinalis leaves, Allium sativum bulb, and Syzygium aromaticum bud inhibited the growth of E. faecalis. Methanol extracts of A. sativum and methanol and acetone extracts of S. aromaticum significantly reduced the mortality of fish artificially infected with E. faecalis as both preventive and therapeutic agents. This is the first report on molecular identification, and herbal control of fish pathogenic E. faecalis in Bangladesh.Muntasir RahmanMd. Mahbubur RahmanSuzan Chandra DebMd. Shahanoor AlamMd. Jahangir AlamMd. Tofazzal IslamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Muntasir Rahman
Md. Mahbubur Rahman
Suzan Chandra Deb
Md. Shahanoor Alam
Md. Jahangir Alam
Md. Tofazzal Islam
Molecular Identification of Multiple Antibiotic Resistant Fish Pathogenic Enterococcus faecalis and their Control by Medicinal Herbs
description Abstract The opportunistic fish pathogen, Enterococcus faecalis has been reported to cause mass mortality in several fish species in different countries. The objectives of this study were to (i) identify E. faecalis from the diseased fishes through molecular techniques; (ii) assess the antibiotic susceptibility profile of E. faecalis isolates; and (iii) control disease in tilapia fish by treatment with medicinal plant extracts. A total of 48 isolates were phenotypically identified as Enterococcus species from tilapia, stinging catfish and walking catfish cultivated in several fish farms in Gazipur. Ten randomly selected isolates were identified as E. faecalis by 16S rRNA gene sequencing. Artificial infection revealed that most of the isolates caused moderate to high mortality in fishes with characteristic disease symptoms. These isolates exhibited resistance to multiple antibiotics in vitro. Bioassay revealed that organic extracts of Tamarindus indica and Emblica officinalis leaves, Allium sativum bulb, and Syzygium aromaticum bud inhibited the growth of E. faecalis. Methanol extracts of A. sativum and methanol and acetone extracts of S. aromaticum significantly reduced the mortality of fish artificially infected with E. faecalis as both preventive and therapeutic agents. This is the first report on molecular identification, and herbal control of fish pathogenic E. faecalis in Bangladesh.
format article
author Muntasir Rahman
Md. Mahbubur Rahman
Suzan Chandra Deb
Md. Shahanoor Alam
Md. Jahangir Alam
Md. Tofazzal Islam
author_facet Muntasir Rahman
Md. Mahbubur Rahman
Suzan Chandra Deb
Md. Shahanoor Alam
Md. Jahangir Alam
Md. Tofazzal Islam
author_sort Muntasir Rahman
title Molecular Identification of Multiple Antibiotic Resistant Fish Pathogenic Enterococcus faecalis and their Control by Medicinal Herbs
title_short Molecular Identification of Multiple Antibiotic Resistant Fish Pathogenic Enterococcus faecalis and their Control by Medicinal Herbs
title_full Molecular Identification of Multiple Antibiotic Resistant Fish Pathogenic Enterococcus faecalis and their Control by Medicinal Herbs
title_fullStr Molecular Identification of Multiple Antibiotic Resistant Fish Pathogenic Enterococcus faecalis and their Control by Medicinal Herbs
title_full_unstemmed Molecular Identification of Multiple Antibiotic Resistant Fish Pathogenic Enterococcus faecalis and their Control by Medicinal Herbs
title_sort molecular identification of multiple antibiotic resistant fish pathogenic enterococcus faecalis and their control by medicinal herbs
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/eacfa5cf7946406390db51810d3ded96
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