Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae

Abstract Anisakidae, marine nematodes, are underrecognized fish-borne zoonotic parasites. Studies on factors that could trigger parasites to actively migrate out of the fish are very limited. The objective of this study was to assess the impact of different environmental conditions (temperature, CO2...

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Autores principales:	Aiyan Guan, Inge Van Damme, Frank Devlieghere, Sarah Gabriël
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ead6cdbd500e472eb4aa13ce9e4cdb6c
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spelling	oai:doaj.org-article:ead6cdbd500e472eb4aa13ce9e4cdb6c2021-12-02T10:54:22ZEffect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae10.1038/s41598-021-83505-52045-2322https://doaj.org/article/ead6cdbd500e472eb4aa13ce9e4cdb6c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83505-5https://doaj.org/toc/2045-2322Abstract Anisakidae, marine nematodes, are underrecognized fish-borne zoonotic parasites. Studies on factors that could trigger parasites to actively migrate out of the fish are very limited. The objective of this study was to assess the impact of different environmental conditions (temperature, CO2 and O2) on larval motility (in situ movement) and mobility (migration) in vitro. Larvae were collected by candling or enzymatic digestion from infected fish, identified morphologically and confirmed molecularly. Individual larvae were transferred to a semi-solid Phosphate Buffered Saline agar, and subjected to different temperatures (6 ℃, 12 ℃, 22 ℃, 37 ℃) at air conditions. Moreover, different combinations of CO2 and O2 with N2 as filler were tested, at both 6 °C and 12 °C. Video recordings of larvae were translated into scores for larval motility and mobility. Results showed that temperature had significant influence on larval movements, with the highest motility and mobility observed at 22 ℃ for Anisakis spp. larvae and 37 ℃ for Pseudoterranova spp. larvae. During the first 10 min, the median migration of Anisakis spp. larvae was 10 cm at 22 ℃, and the median migration of Pseudoterranova spp. larvae was 3 cm at 37 ℃. Larval mobility was not significantly different under the different CO2 or O2 conditions at 6 °C and 12 ℃. It was concluded that temperature significantly facilitated larval movement with the optimum temperature being different for Anisakis spp. and Pseudoterranova spp., while CO2 and O2 did not on the short term. This should be further validated in parasite-infected/spiked fish fillets.Aiyan GuanInge Van DammeFrank DevlieghereSarah GabriëlNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aiyan Guan Inge Van Damme Frank Devlieghere Sarah Gabriël Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae
description	Abstract Anisakidae, marine nematodes, are underrecognized fish-borne zoonotic parasites. Studies on factors that could trigger parasites to actively migrate out of the fish are very limited. The objective of this study was to assess the impact of different environmental conditions (temperature, CO2 and O2) on larval motility (in situ movement) and mobility (migration) in vitro. Larvae were collected by candling or enzymatic digestion from infected fish, identified morphologically and confirmed molecularly. Individual larvae were transferred to a semi-solid Phosphate Buffered Saline agar, and subjected to different temperatures (6 ℃, 12 ℃, 22 ℃, 37 ℃) at air conditions. Moreover, different combinations of CO2 and O2 with N2 as filler were tested, at both 6 °C and 12 °C. Video recordings of larvae were translated into scores for larval motility and mobility. Results showed that temperature had significant influence on larval movements, with the highest motility and mobility observed at 22 ℃ for Anisakis spp. larvae and 37 ℃ for Pseudoterranova spp. larvae. During the first 10 min, the median migration of Anisakis spp. larvae was 10 cm at 22 ℃, and the median migration of Pseudoterranova spp. larvae was 3 cm at 37 ℃. Larval mobility was not significantly different under the different CO2 or O2 conditions at 6 °C and 12 ℃. It was concluded that temperature significantly facilitated larval movement with the optimum temperature being different for Anisakis spp. and Pseudoterranova spp., while CO2 and O2 did not on the short term. This should be further validated in parasite-infected/spiked fish fillets.
format	article
author	Aiyan Guan Inge Van Damme Frank Devlieghere Sarah Gabriël
author_facet	Aiyan Guan Inge Van Damme Frank Devlieghere Sarah Gabriël
author_sort	Aiyan Guan
title	Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae
title_short	Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae
title_full	Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae
title_fullStr	Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae
title_full_unstemmed	Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae
title_sort	effect of temperature, co2 and o2 on motility and mobility of anisakidae larvae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ead6cdbd500e472eb4aa13ce9e4cdb6c
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Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae

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