Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA

Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA

Engineered high-fidelity Cas9s have increased discrimination against off-targets. Kinetic analyses of Cas9-HF1 and HypaCas9 engineered Cas9 variants show that their DNA cleavage is impaired by more than 100- fold, which leads to release rather than cleavage of a bound off-target substrate.

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Autores principales: Mu-Sen Liu, Shanzhong Gong, Helen-Hong Yu, Kyungseok Jung, Kenneth A. Johnson, David W. Taylor
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/eaec27d1773d4739aca435216526ab4f
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spelling oai:doaj.org-article:eaec27d1773d4739aca435216526ab4f2021-12-02T18:36:59ZEngineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA10.1038/s41467-020-17411-12041-1723https://doaj.org/article/eaec27d1773d4739aca435216526ab4f2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17411-1https://doaj.org/toc/2041-1723Engineered high-fidelity Cas9s have increased discrimination against off-targets. Kinetic analyses of Cas9-HF1 and HypaCas9 engineered Cas9 variants show that their DNA cleavage is impaired by more than 100- fold, which leads to release rather than cleavage of a bound off-target substrate.Mu-Sen LiuShanzhong GongHelen-Hong YuKyungseok JungKenneth A. JohnsonDavid W. TaylorNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Mu-Sen Liu
Shanzhong Gong
Helen-Hong Yu
Kyungseok Jung
Kenneth A. Johnson
David W. Taylor
Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
description Engineered high-fidelity Cas9s have increased discrimination against off-targets. Kinetic analyses of Cas9-HF1 and HypaCas9 engineered Cas9 variants show that their DNA cleavage is impaired by more than 100- fold, which leads to release rather than cleavage of a bound off-target substrate.
format article
author Mu-Sen Liu
Shanzhong Gong
Helen-Hong Yu
Kyungseok Jung
Kenneth A. Johnson
David W. Taylor
author_facet Mu-Sen Liu
Shanzhong Gong
Helen-Hong Yu
Kyungseok Jung
Kenneth A. Johnson
David W. Taylor
author_sort Mu-Sen Liu
title Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
title_short Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
title_full Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
title_fullStr Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
title_full_unstemmed Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
title_sort engineered crispr/cas9 enzymes improve discrimination by slowing dna cleavage to allow release of off-target dna
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/eaec27d1773d4739aca435216526ab4f
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AT shanzhonggong engineeredcrisprcas9enzymesimprovediscriminationbyslowingdnacleavagetoallowreleaseofofftargetdna
AT helenhongyu engineeredcrisprcas9enzymesimprovediscriminationbyslowingdnacleavagetoallowreleaseofofftargetdna
AT kyungseokjung engineeredcrisprcas9enzymesimprovediscriminationbyslowingdnacleavagetoallowreleaseofofftargetdna
AT kennethajohnson engineeredcrisprcas9enzymesimprovediscriminationbyslowingdnacleavagetoallowreleaseofofftargetdna
AT davidwtaylor engineeredcrisprcas9enzymesimprovediscriminationbyslowingdnacleavagetoallowreleaseofofftargetdna
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