Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes

Volkan Mujdat Tiryaki,1 Virginia M Ayres,1 Adeel A Khan,2 Ijaz Ahmed,3 David I Shreiber,3 Sally Meiners41Electronic and Biological Nanostructures Laboratory, Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, USA; 2Department of Paper Engineering, Chemica...

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Autores principales:	Tiryaki VM, Ayres VM, Khan AA, Ahmed I, Shreiber DI, Meiners S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eaf10fc814624271a10d6bbbda120fb8
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spelling	oai:doaj.org-article:eaf10fc814624271a10d6bbbda120fb82021-12-02T05:09:48ZNanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes1176-91141178-2013https://doaj.org/article/eaf10fc814624271a10d6bbbda120fb82012-07-01T00:00:00Zhttp://www.dovepress.com/nanofibrillar-scaffolds-induce-preferential-activation-of-rho-gtpases--a10474https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Volkan Mujdat Tiryaki,1 Virginia M Ayres,1 Adeel A Khan,2 Ijaz Ahmed,3 David I Shreiber,3 Sally Meiners41Electronic and Biological Nanostructures Laboratory, Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, USA; 2Department of Paper Engineering, Chemical Engineering, Imaging, Western Michigan University, Kalamazoo, MI, USA; 3Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, USA; 4Nanoculture, LLC, Piscataway, NJ, USAAbstract: Cerebral cortical astrocyte responses to polyamide nanofibrillar scaffolds versus poly-L-lysine (PLL)-functionalized planar glass, unfunctionalized planar Aclar coverslips, and PLL-functionalized planar Aclar surfaces were investigated by atomic force microscopy and immunocytochemistry. The physical properties of the cell culture environments were evaluated using contact angle and surface roughness measurements and compared. Astrocyte morphological responses, including filopodia, lamellipodia, and stress fiber formation, and stellation were imaged using atomic force microscopy and phalloidin staining for F-actin. Activation of the corresponding Rho GTPase regulators was investigated using immunolabeling with Cdc42, Rac1, and RhoA. Astrocytes cultured on the nanofibrillar scaffolds showed a unique response that included stellation, cell&ndash;cell interactions by stellate processes, and evidence of depression of RhoA. The results support the hypothesis that the extracellular environment can trigger preferential activation of members of the Rho GTPase family, with demonstrable morphological consequences for cerebral cortical astrocytes.Keywords: stellation, nanofiber, RhoA, atomic force microscopyTiryaki VMAyres VMKhan AAAhmed IShreiber DIMeiners SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3891-3905 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Tiryaki VM Ayres VM Khan AA Ahmed I Shreiber DI Meiners S Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes
description	Volkan Mujdat Tiryaki,1 Virginia M Ayres,1 Adeel A Khan,2 Ijaz Ahmed,3 David I Shreiber,3 Sally Meiners41Electronic and Biological Nanostructures Laboratory, Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, USA; 2Department of Paper Engineering, Chemical Engineering, Imaging, Western Michigan University, Kalamazoo, MI, USA; 3Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, USA; 4Nanoculture, LLC, Piscataway, NJ, USAAbstract: Cerebral cortical astrocyte responses to polyamide nanofibrillar scaffolds versus poly-L-lysine (PLL)-functionalized planar glass, unfunctionalized planar Aclar coverslips, and PLL-functionalized planar Aclar surfaces were investigated by atomic force microscopy and immunocytochemistry. The physical properties of the cell culture environments were evaluated using contact angle and surface roughness measurements and compared. Astrocyte morphological responses, including filopodia, lamellipodia, and stress fiber formation, and stellation were imaged using atomic force microscopy and phalloidin staining for F-actin. Activation of the corresponding Rho GTPase regulators was investigated using immunolabeling with Cdc42, Rac1, and RhoA. Astrocytes cultured on the nanofibrillar scaffolds showed a unique response that included stellation, cell&ndash;cell interactions by stellate processes, and evidence of depression of RhoA. The results support the hypothesis that the extracellular environment can trigger preferential activation of members of the Rho GTPase family, with demonstrable morphological consequences for cerebral cortical astrocytes.Keywords: stellation, nanofiber, RhoA, atomic force microscopy
format	article
author	Tiryaki VM Ayres VM Khan AA Ahmed I Shreiber DI Meiners S
author_facet	Tiryaki VM Ayres VM Khan AA Ahmed I Shreiber DI Meiners S
author_sort	Tiryaki VM
title	Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes
title_short	Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes
title_full	Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes
title_fullStr	Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes
title_full_unstemmed	Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes
title_sort	nanofibrillar scaffolds induce preferential activation of rho gtpases in cerebral cortical astrocytes
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/eaf10fc814624271a10d6bbbda120fb8
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Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes

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