A new method for sampling African swine fever virus genome and its inactivation in environmental samples

Abstract African swine fever (ASF) is currently the most dangerous disease for the global pig industry, causing huge economic losses, due to the lack of effective vaccine or treatment. Only the early detection of ASF virus (ASFV) and proper biosecurity measures are effective to reduce the viral expa...

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Autores principales:	Aleksandra Kosowska, Jose A. Barasona, Sandra Barroso-Arévalo, Belén Rivera, Lucas Domínguez, Jose M. Sánchez-Vizcaíno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eafd98b582a14e7bb032e53a5a6dd4db
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spelling	oai:doaj.org-article:eafd98b582a14e7bb032e53a5a6dd4db2021-11-08T10:47:59ZA new method for sampling African swine fever virus genome and its inactivation in environmental samples10.1038/s41598-021-00552-82045-2322https://doaj.org/article/eafd98b582a14e7bb032e53a5a6dd4db2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00552-8https://doaj.org/toc/2045-2322Abstract African swine fever (ASF) is currently the most dangerous disease for the global pig industry, causing huge economic losses, due to the lack of effective vaccine or treatment. Only the early detection of ASF virus (ASFV) and proper biosecurity measures are effective to reduce the viral expansion. One of the most widely recognized risks as regards the introduction ASFV into a country is infected animals and contaminated livestock vehicles. In order to improve ASF surveillance, we have assessed the capacity for the detection and inactivation of ASFV genome by using Dry-Sponges (3 M) pre-hydrated with a new surfactant liquid. We sampled different surfaces in ASFV-contaminated facilities, including animal skins, and the results were compared to those obtained using a traditional sampling method. The surfactant liquid successfully inactivated the virus, while ASFV DNA was well preserved for the detection. This is an effective method to systematically recover ASFV DNA from different surfaces and skin, which has a key applied relevance in surveillance of vehicles transporting live animals and greatly improves animal welfare. This method provides an important basis for the detection of ASFV genome that can be assessed without the biosafety requirements of a BSL-3 laboratory at least in ASF-affected countries, which may substantially speed up the early detection of the pathogen.Aleksandra KosowskaJose A. BarasonaSandra Barroso-ArévaloBelén RiveraLucas DomínguezJose M. Sánchez-VizcaínoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aleksandra Kosowska Jose A. Barasona Sandra Barroso-Arévalo Belén Rivera Lucas Domínguez Jose M. Sánchez-Vizcaíno A new method for sampling African swine fever virus genome and its inactivation in environmental samples
description	Abstract African swine fever (ASF) is currently the most dangerous disease for the global pig industry, causing huge economic losses, due to the lack of effective vaccine or treatment. Only the early detection of ASF virus (ASFV) and proper biosecurity measures are effective to reduce the viral expansion. One of the most widely recognized risks as regards the introduction ASFV into a country is infected animals and contaminated livestock vehicles. In order to improve ASF surveillance, we have assessed the capacity for the detection and inactivation of ASFV genome by using Dry-Sponges (3 M) pre-hydrated with a new surfactant liquid. We sampled different surfaces in ASFV-contaminated facilities, including animal skins, and the results were compared to those obtained using a traditional sampling method. The surfactant liquid successfully inactivated the virus, while ASFV DNA was well preserved for the detection. This is an effective method to systematically recover ASFV DNA from different surfaces and skin, which has a key applied relevance in surveillance of vehicles transporting live animals and greatly improves animal welfare. This method provides an important basis for the detection of ASFV genome that can be assessed without the biosafety requirements of a BSL-3 laboratory at least in ASF-affected countries, which may substantially speed up the early detection of the pathogen.
format	article
author	Aleksandra Kosowska Jose A. Barasona Sandra Barroso-Arévalo Belén Rivera Lucas Domínguez Jose M. Sánchez-Vizcaíno
author_facet	Aleksandra Kosowska Jose A. Barasona Sandra Barroso-Arévalo Belén Rivera Lucas Domínguez Jose M. Sánchez-Vizcaíno
author_sort	Aleksandra Kosowska
title	A new method for sampling African swine fever virus genome and its inactivation in environmental samples
title_short	A new method for sampling African swine fever virus genome and its inactivation in environmental samples
title_full	A new method for sampling African swine fever virus genome and its inactivation in environmental samples
title_fullStr	A new method for sampling African swine fever virus genome and its inactivation in environmental samples
title_full_unstemmed	A new method for sampling African swine fever virus genome and its inactivation in environmental samples
title_sort	new method for sampling african swine fever virus genome and its inactivation in environmental samples
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eafd98b582a14e7bb032e53a5a6dd4db
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A new method for sampling African swine fever virus genome and its inactivation in environmental samples

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