Lipid pathway alterations in Parkinson's disease primary visual cortex.

<h4>Background</h4>We present a lipidomics analysis of human Parkinson's disease tissues. We have focused on the primary visual cortex, a region that is devoid of pathological changes and Lewy bodies; and two additional regions, the amygdala and anterior cingulate cortex which conta...

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Autores principales: Danni Cheng, Andrew M Jenner, Guanghou Shui, Wei Fun Cheong, Todd W Mitchell, Jessica R Nealon, Woojin S Kim, Heather McCann, Markus R Wenk, Glenda M Halliday, Brett Garner
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Publicado: Public Library of Science (PLoS) 2011
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spelling oai:doaj.org-article:eb0e9b627a6442e8b6633f95c48d193c2021-11-18T06:58:01ZLipid pathway alterations in Parkinson's disease primary visual cortex.1932-620310.1371/journal.pone.0017299https://doaj.org/article/eb0e9b627a6442e8b6633f95c48d193c2011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21387008/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>We present a lipidomics analysis of human Parkinson's disease tissues. We have focused on the primary visual cortex, a region that is devoid of pathological changes and Lewy bodies; and two additional regions, the amygdala and anterior cingulate cortex which contain Lewy bodies at different disease stages but do not have as severe degeneration as the substantia nigra.<h4>Methodology/principal findings</h4>Using liquid chromatography mass spectrometry lipidomics techniques for an initial screen of 200 lipid species, significant changes in 79 sphingolipid, glycerophospholipid and cholesterol species were detected in the visual cortex of Parkinson's disease patients (n = 10) compared to controls (n = 10) as assessed by two-sided unpaired t-test (p-value <0.05). False discovery rate analysis confirmed that 73 of these 79 lipid species were significantly changed in the visual cortex (q-value <0.05). By contrast, changes in 17 and 12 lipid species were identified in the Parkinson's disease amygdala and anterior cingulate cortex, respectively, compared to controls; none of which remained significant after false discovery rate analysis. Using gas chromatography mass spectrometry techniques, 6 out of 7 oxysterols analysed from both non-enzymatic and enzymatic pathways were also selectively increased in the Parkinson's disease visual cortex. Many of these changes in visual cortex lipids were correlated with relevant changes in the expression of genes involved in lipid metabolism and an oxidative stress response as determined by quantitative polymerase chain reaction techniques.<h4>Conclusions/significance</h4>The data indicate that changes in lipid metabolism occur in the Parkinson's disease visual cortex in the absence of obvious pathology. This suggests that normalization of lipid metabolism and/or oxidative stress status in the visual cortex may represent a novel route for treatment of non-motor symptoms, such as visual hallucinations, that are experienced by a majority of Parkinson's disease patients.Danni ChengAndrew M JennerGuanghou ShuiWei Fun CheongTodd W MitchellJessica R NealonWoojin S KimHeather McCannMarkus R WenkGlenda M HallidayBrett GarnerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e17299 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Danni Cheng
Andrew M Jenner
Guanghou Shui
Wei Fun Cheong
Todd W Mitchell
Jessica R Nealon
Woojin S Kim
Heather McCann
Markus R Wenk
Glenda M Halliday
Brett Garner
Lipid pathway alterations in Parkinson's disease primary visual cortex.
description <h4>Background</h4>We present a lipidomics analysis of human Parkinson's disease tissues. We have focused on the primary visual cortex, a region that is devoid of pathological changes and Lewy bodies; and two additional regions, the amygdala and anterior cingulate cortex which contain Lewy bodies at different disease stages but do not have as severe degeneration as the substantia nigra.<h4>Methodology/principal findings</h4>Using liquid chromatography mass spectrometry lipidomics techniques for an initial screen of 200 lipid species, significant changes in 79 sphingolipid, glycerophospholipid and cholesterol species were detected in the visual cortex of Parkinson's disease patients (n = 10) compared to controls (n = 10) as assessed by two-sided unpaired t-test (p-value <0.05). False discovery rate analysis confirmed that 73 of these 79 lipid species were significantly changed in the visual cortex (q-value <0.05). By contrast, changes in 17 and 12 lipid species were identified in the Parkinson's disease amygdala and anterior cingulate cortex, respectively, compared to controls; none of which remained significant after false discovery rate analysis. Using gas chromatography mass spectrometry techniques, 6 out of 7 oxysterols analysed from both non-enzymatic and enzymatic pathways were also selectively increased in the Parkinson's disease visual cortex. Many of these changes in visual cortex lipids were correlated with relevant changes in the expression of genes involved in lipid metabolism and an oxidative stress response as determined by quantitative polymerase chain reaction techniques.<h4>Conclusions/significance</h4>The data indicate that changes in lipid metabolism occur in the Parkinson's disease visual cortex in the absence of obvious pathology. This suggests that normalization of lipid metabolism and/or oxidative stress status in the visual cortex may represent a novel route for treatment of non-motor symptoms, such as visual hallucinations, that are experienced by a majority of Parkinson's disease patients.
format article
author Danni Cheng
Andrew M Jenner
Guanghou Shui
Wei Fun Cheong
Todd W Mitchell
Jessica R Nealon
Woojin S Kim
Heather McCann
Markus R Wenk
Glenda M Halliday
Brett Garner
author_facet Danni Cheng
Andrew M Jenner
Guanghou Shui
Wei Fun Cheong
Todd W Mitchell
Jessica R Nealon
Woojin S Kim
Heather McCann
Markus R Wenk
Glenda M Halliday
Brett Garner
author_sort Danni Cheng
title Lipid pathway alterations in Parkinson's disease primary visual cortex.
title_short Lipid pathway alterations in Parkinson's disease primary visual cortex.
title_full Lipid pathway alterations in Parkinson's disease primary visual cortex.
title_fullStr Lipid pathway alterations in Parkinson's disease primary visual cortex.
title_full_unstemmed Lipid pathway alterations in Parkinson's disease primary visual cortex.
title_sort lipid pathway alterations in parkinson's disease primary visual cortex.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/eb0e9b627a6442e8b6633f95c48d193c
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