Rabbit as a novel animal model for hepatitis E virus infection and vaccine evaluation.

<h4>Background</h4>The identification of hepatitis E virus (HEV) from rabbits motivated us to assess the possibility of using rabbits as a non-human primate animal model for HEV infection and vaccine evaluation.<h4>Methodology/principal findings</h4>First, 75 rabbits were ino...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xianfeng Cheng, Song Wang, Xing Dai, Chengbo Shi, Yufeng Wen, Ming Zhu, Shenwei Zhan, Jihong Meng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb13128ebb884528bc98a261ca0a60ea
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eb13128ebb884528bc98a261ca0a60ea
record_format	dspace
spelling	oai:doaj.org-article:eb13128ebb884528bc98a261ca0a60ea2021-11-18T08:05:12ZRabbit as a novel animal model for hepatitis E virus infection and vaccine evaluation.1932-620310.1371/journal.pone.0051616https://doaj.org/article/eb13128ebb884528bc98a261ca0a60ea2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23272124/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The identification of hepatitis E virus (HEV) from rabbits motivated us to assess the possibility of using rabbits as a non-human primate animal model for HEV infection and vaccine evaluation.<h4>Methodology/principal findings</h4>First, 75 rabbits were inoculated with seven strains of genotypes 1, 3, 4, and rabbit HEV, to determine the appropriate strain, administrative route and viral dosage. Second, 15 rabbits were randomly divided into three groups and vaccinated with 0 µg (placebo), 10 µg and 20 µg of HEV candidate vaccine, HEV p179, respectively. After three doses of the vaccination, the rabbits were challenged with 3.3×10(5) genome equivalents of genotype 4 HEV strain H4-NJ703. The strain of genotype 1 HEV was not found to be infectious for rabbits. However, approximately 80% of the animals were infected by two rabbit HEV strains. All rabbits inoculated with a genotype 3 strain were seroconverted but did not show viremia or fecal viral shedding. Although two genotype 4 strains, H4-NJ153 and H4-NJ112, only resulted in part of rabbits infected, another strain of genotype 4, H4-NJ703, had an infection rate of 100% (five out of five) when administrated intravenously. However, only two out of fifteen rabbits showed virus excretion and seroconversion when inoculated orally with H4-NJ703 of three different dosages. In the vaccine evaluation study, rabbits vaccinated with 20 µg of the HEV p179 produced anti-HEV with titers of 1∶10(4)-1∶10(5) and were completely protected from infection. Rabbits vaccinated with 10 µg produced anti-HEV with titers of 1∶10(3)-1∶10(4) and were protected from hepatitis, but two out of the five rabbits showed virus shedding.<h4>Conclusions/significance</h4>Rabbits may be served as an alternative to the non-human primate models for HEV infection and vaccine evaluation when certain virus strains, appropriate viral dosages, and the intravenous route of inoculation are selected.Xianfeng ChengSong WangXing DaiChengbo ShiYufeng WenMing ZhuShenwei ZhanJihong MengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51616 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xianfeng Cheng Song Wang Xing Dai Chengbo Shi Yufeng Wen Ming Zhu Shenwei Zhan Jihong Meng Rabbit as a novel animal model for hepatitis E virus infection and vaccine evaluation.
description	<h4>Background</h4>The identification of hepatitis E virus (HEV) from rabbits motivated us to assess the possibility of using rabbits as a non-human primate animal model for HEV infection and vaccine evaluation.<h4>Methodology/principal findings</h4>First, 75 rabbits were inoculated with seven strains of genotypes 1, 3, 4, and rabbit HEV, to determine the appropriate strain, administrative route and viral dosage. Second, 15 rabbits were randomly divided into three groups and vaccinated with 0 µg (placebo), 10 µg and 20 µg of HEV candidate vaccine, HEV p179, respectively. After three doses of the vaccination, the rabbits were challenged with 3.3×10(5) genome equivalents of genotype 4 HEV strain H4-NJ703. The strain of genotype 1 HEV was not found to be infectious for rabbits. However, approximately 80% of the animals were infected by two rabbit HEV strains. All rabbits inoculated with a genotype 3 strain were seroconverted but did not show viremia or fecal viral shedding. Although two genotype 4 strains, H4-NJ153 and H4-NJ112, only resulted in part of rabbits infected, another strain of genotype 4, H4-NJ703, had an infection rate of 100% (five out of five) when administrated intravenously. However, only two out of fifteen rabbits showed virus excretion and seroconversion when inoculated orally with H4-NJ703 of three different dosages. In the vaccine evaluation study, rabbits vaccinated with 20 µg of the HEV p179 produced anti-HEV with titers of 1∶10(4)-1∶10(5) and were completely protected from infection. Rabbits vaccinated with 10 µg produced anti-HEV with titers of 1∶10(3)-1∶10(4) and were protected from hepatitis, but two out of the five rabbits showed virus shedding.<h4>Conclusions/significance</h4>Rabbits may be served as an alternative to the non-human primate models for HEV infection and vaccine evaluation when certain virus strains, appropriate viral dosages, and the intravenous route of inoculation are selected.
format	article
author	Xianfeng Cheng Song Wang Xing Dai Chengbo Shi Yufeng Wen Ming Zhu Shenwei Zhan Jihong Meng
author_facet	Xianfeng Cheng Song Wang Xing Dai Chengbo Shi Yufeng Wen Ming Zhu Shenwei Zhan Jihong Meng
author_sort	Xianfeng Cheng
title	Rabbit as a novel animal model for hepatitis E virus infection and vaccine evaluation.
title_short	Rabbit as a novel animal model for hepatitis E virus infection and vaccine evaluation.
title_full	Rabbit as a novel animal model for hepatitis E virus infection and vaccine evaluation.
title_fullStr	Rabbit as a novel animal model for hepatitis E virus infection and vaccine evaluation.
title_full_unstemmed	Rabbit as a novel animal model for hepatitis E virus infection and vaccine evaluation.
title_sort	rabbit as a novel animal model for hepatitis e virus infection and vaccine evaluation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/eb13128ebb884528bc98a261ca0a60ea
work_keys_str_mv	AT xianfengcheng rabbitasanovelanimalmodelforhepatitisevirusinfectionandvaccineevaluation AT songwang rabbitasanovelanimalmodelforhepatitisevirusinfectionandvaccineevaluation AT xingdai rabbitasanovelanimalmodelforhepatitisevirusinfectionandvaccineevaluation AT chengboshi rabbitasanovelanimalmodelforhepatitisevirusinfectionandvaccineevaluation AT yufengwen rabbitasanovelanimalmodelforhepatitisevirusinfectionandvaccineevaluation AT mingzhu rabbitasanovelanimalmodelforhepatitisevirusinfectionandvaccineevaluation AT shenweizhan rabbitasanovelanimalmodelforhepatitisevirusinfectionandvaccineevaluation AT jihongmeng rabbitasanovelanimalmodelforhepatitisevirusinfectionandvaccineevaluation
_version_	1718422263713234944

Rabbit as a novel animal model for hepatitis E virus infection and vaccine evaluation.

Ejemplares similares