Salmonella-induced changes in the level of key immunoregulatory bacteria affect the transcriptional activity of the <i>Foxp3</i> and <i>RORgt</i> genes in the gut-associated lymphoid tissue of rats

Intestinal microbes involved in many physiological processes owner, contributes to the formation and maintenance of immune homeostasis by regulating immune responses to protect against colonization by pathogens. A special role in the differentiation of various subpopulations of T-lymphocytes play th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yu. V. Bukina, L. Ya. Fedoniuk, G. D. Koval, Yu. O. Shekhovtsova, A. M. Kamyshnyi, A. O. Gubar, V. O. Gubka
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2020
Materias:	microbiome gut-associated lymphoid tissue salmonella vancomycin bacteroides expression rt-pcr Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/eb165809fe7641d5b3e859cc6cf7e85f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eb165809fe7641d5b3e859cc6cf7e85f
record_format	dspace
spelling	oai:doaj.org-article:eb165809fe7641d5b3e859cc6cf7e85f2021-11-22T07:09:55ZSalmonella-induced changes in the level of key immunoregulatory bacteria affect the transcriptional activity of the <i>Foxp3</i> and <i>RORgt</i> genes in the gut-associated lymphoid tissue of rats2220-76192313-739810.15789/2220-7619-SIC-1151https://doaj.org/article/eb165809fe7641d5b3e859cc6cf7e85f2020-11-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1151https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Intestinal microbes involved in many physiological processes owner, contributes to the formation and maintenance of immune homeostasis by regulating immune responses to protect against colonization by pathogens. A special role in the differentiation of various subpopulations of T-lymphocytes play the segmental filamentous bacteria (Segmented filamentous bacteria, SFB), capable of inducing a gut-associated lymphoid tissue (GALT) differentiation proinflammatory Th17-cells and members of the genus Clostridium (cluster IV and XIVa) and Bacteroides fragilis (polysaccharide A [PSA]), stimulating the formation of regulatory T-cells (Treg) and production of suppressor of cytokine IL-10. Important metabolites of B. fragilis are short-chain fatty acids (SCFA), which are able to activate GALT cells through the FFAR2 receptor. Lowering of the SCFA concentration leads to the reduction of the number of Treg in the intestine and breaks Th17/Treg balance. These changes lead to direct reducing of mRNA FFAR2, Foxp3 expression and increasing in RORyt GALT. Therefore, the goal was to determine the level of the key in the edge immunoregulatory bacteria intestinal microflora rats and their effects on the transcriptional activity of the genes Foxp3 and RORyt in GALT with Salmonella-induced inflammation and during administration of vancomycin and B. fragilis. To determine the genus and species of bacteria, as well as their number in the microflora of rats, was used the method of polymerase chain reaction (PCR-RV) with their identification by 16S rDNA genes. To study the transcriptional activity of genes using polymerase chain reaction reverse transcription real-time (RT-PCR). During the experiment with the introduction of animals vancomycin and Salmonella there was an increase in the level of SFB and a decrease in A. muciniphila, F. prausnitzii. Also, during infecting rats with S. Enteritidis and S. Typhimurium on the background of pre-treatment with vancomycin, there was an increase in the number of SFBs against the background of a pronounced decrease in Bacteroides—Prevotela group, A. muciniphila, Clostridium spp. clusters XIV, IV, and F. prausnitzii, which led to a decrease in the expression level of Foxp3+ mRNA and an increase in RORyt+, respectively. However, administration of B. fragilis to animals receiving S. Enteritidis or S. Typhimurium against pretreatment with vancomycin caused a decrease in the level of SFB and mRNA RORyt+, and, conversely, increased the number of Bacteroides—Prevotela group, A. muciniphila, Clostridium spp. clusters XIV, IV, F. prausnitzii and expression of Foxp3+ genes, which indicates the restoration of the homeostasis of the intestinal microbiome. The obtained results showed that B. fragilis can be successfully used in the treatment of inflammatory bowel diseases or diseases with impaired intestinal barrier function.Yu. V. BukinaL. Ya. FedoniukG. D. KovalYu. O. ShekhovtsovaA. M. KamyshnyiA. O. GubarV. O. GubkaSankt-Peterburg : NIIÈM imeni Pasteraarticlemicrobiomegut-associated lymphoid tissuesalmonellavancomycinbacteroidesexpressionrt-pcrInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 10, Iss 4, Pp 671-685 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	microbiome gut-associated lymphoid tissue salmonella vancomycin bacteroides expression rt-pcr Infectious and parasitic diseases RC109-216
spellingShingle	microbiome gut-associated lymphoid tissue salmonella vancomycin bacteroides expression rt-pcr Infectious and parasitic diseases RC109-216 Yu. V. Bukina L. Ya. Fedoniuk G. D. Koval Yu. O. Shekhovtsova A. M. Kamyshnyi A. O. Gubar V. O. Gubka Salmonella-induced changes in the level of key immunoregulatory bacteria affect the transcriptional activity of the <i>Foxp3</i> and <i>RORgt</i> genes in the gut-associated lymphoid tissue of rats
description	Intestinal microbes involved in many physiological processes owner, contributes to the formation and maintenance of immune homeostasis by regulating immune responses to protect against colonization by pathogens. A special role in the differentiation of various subpopulations of T-lymphocytes play the segmental filamentous bacteria (Segmented filamentous bacteria, SFB), capable of inducing a gut-associated lymphoid tissue (GALT) differentiation proinflammatory Th17-cells and members of the genus Clostridium (cluster IV and XIVa) and Bacteroides fragilis (polysaccharide A [PSA]), stimulating the formation of regulatory T-cells (Treg) and production of suppressor of cytokine IL-10. Important metabolites of B. fragilis are short-chain fatty acids (SCFA), which are able to activate GALT cells through the FFAR2 receptor. Lowering of the SCFA concentration leads to the reduction of the number of Treg in the intestine and breaks Th17/Treg balance. These changes lead to direct reducing of mRNA FFAR2, Foxp3 expression and increasing in RORyt GALT. Therefore, the goal was to determine the level of the key in the edge immunoregulatory bacteria intestinal microflora rats and their effects on the transcriptional activity of the genes Foxp3 and RORyt in GALT with Salmonella-induced inflammation and during administration of vancomycin and B. fragilis. To determine the genus and species of bacteria, as well as their number in the microflora of rats, was used the method of polymerase chain reaction (PCR-RV) with their identification by 16S rDNA genes. To study the transcriptional activity of genes using polymerase chain reaction reverse transcription real-time (RT-PCR). During the experiment with the introduction of animals vancomycin and Salmonella there was an increase in the level of SFB and a decrease in A. muciniphila, F. prausnitzii. Also, during infecting rats with S. Enteritidis and S. Typhimurium on the background of pre-treatment with vancomycin, there was an increase in the number of SFBs against the background of a pronounced decrease in Bacteroides—Prevotela group, A. muciniphila, Clostridium spp. clusters XIV, IV, and F. prausnitzii, which led to a decrease in the expression level of Foxp3+ mRNA and an increase in RORyt+, respectively. However, administration of B. fragilis to animals receiving S. Enteritidis or S. Typhimurium against pretreatment with vancomycin caused a decrease in the level of SFB and mRNA RORyt+, and, conversely, increased the number of Bacteroides—Prevotela group, A. muciniphila, Clostridium spp. clusters XIV, IV, F. prausnitzii and expression of Foxp3+ genes, which indicates the restoration of the homeostasis of the intestinal microbiome. The obtained results showed that B. fragilis can be successfully used in the treatment of inflammatory bowel diseases or diseases with impaired intestinal barrier function.
format	article
author	Yu. V. Bukina L. Ya. Fedoniuk G. D. Koval Yu. O. Shekhovtsova A. M. Kamyshnyi A. O. Gubar V. O. Gubka
author_facet	Yu. V. Bukina L. Ya. Fedoniuk G. D. Koval Yu. O. Shekhovtsova A. M. Kamyshnyi A. O. Gubar V. O. Gubka
author_sort	Yu. V. Bukina
title	Salmonella-induced changes in the level of key immunoregulatory bacteria affect the transcriptional activity of the <i>Foxp3</i> and <i>RORgt</i> genes in the gut-associated lymphoid tissue of rats
title_short	Salmonella-induced changes in the level of key immunoregulatory bacteria affect the transcriptional activity of the <i>Foxp3</i> and <i>RORgt</i> genes in the gut-associated lymphoid tissue of rats
title_full	Salmonella-induced changes in the level of key immunoregulatory bacteria affect the transcriptional activity of the <i>Foxp3</i> and <i>RORgt</i> genes in the gut-associated lymphoid tissue of rats
title_fullStr	Salmonella-induced changes in the level of key immunoregulatory bacteria affect the transcriptional activity of the <i>Foxp3</i> and <i>RORgt</i> genes in the gut-associated lymphoid tissue of rats
title_full_unstemmed	Salmonella-induced changes in the level of key immunoregulatory bacteria affect the transcriptional activity of the <i>Foxp3</i> and <i>RORgt</i> genes in the gut-associated lymphoid tissue of rats
title_sort	salmonella-induced changes in the level of key immunoregulatory bacteria affect the transcriptional activity of the <i>foxp3</i> and <i>rorgt</i> genes in the gut-associated lymphoid tissue of rats
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2020
url	https://doaj.org/article/eb165809fe7641d5b3e859cc6cf7e85f
work_keys_str_mv	AT yuvbukina salmonellainducedchangesinthelevelofkeyimmunoregulatorybacteriaaffectthetranscriptionalactivityoftheifoxp3iandirorgtigenesinthegutassociatedlymphoidtissueofrats AT lyafedoniuk salmonellainducedchangesinthelevelofkeyimmunoregulatorybacteriaaffectthetranscriptionalactivityoftheifoxp3iandirorgtigenesinthegutassociatedlymphoidtissueofrats AT gdkoval salmonellainducedchangesinthelevelofkeyimmunoregulatorybacteriaaffectthetranscriptionalactivityoftheifoxp3iandirorgtigenesinthegutassociatedlymphoidtissueofrats AT yuoshekhovtsova salmonellainducedchangesinthelevelofkeyimmunoregulatorybacteriaaffectthetranscriptionalactivityoftheifoxp3iandirorgtigenesinthegutassociatedlymphoidtissueofrats AT amkamyshnyi salmonellainducedchangesinthelevelofkeyimmunoregulatorybacteriaaffectthetranscriptionalactivityoftheifoxp3iandirorgtigenesinthegutassociatedlymphoidtissueofrats AT aogubar salmonellainducedchangesinthelevelofkeyimmunoregulatorybacteriaaffectthetranscriptionalactivityoftheifoxp3iandirorgtigenesinthegutassociatedlymphoidtissueofrats AT vogubka salmonellainducedchangesinthelevelofkeyimmunoregulatorybacteriaaffectthetranscriptionalactivityoftheifoxp3iandirorgtigenesinthegutassociatedlymphoidtissueofrats
_version_	1718417889532313600

Salmonella-induced changes in the level of key immunoregulatory bacteria affect the transcriptional activity of the <i>Foxp3</i> and <i>RORgt</i> genes in the gut-associated lymphoid tissue of rats

Ejemplares similares