Differences in innate cytokine responses between European and African children.

Although differences in immunological responses between populations have been found in terms of vaccine efficacy, immune responses to infections and prevalence of chronic inflammatory diseases, the mechanisms responsible for these differences are not well understood. Therefore, innate cytokine respo...

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Autores principales:	Lucja A Labuda, Sanne E de Jong, Lynn Meurs, Abena S Amoah, Moustapha Mbow, Ulysse Ateba-Ngoa, Alwin J van der Ham, André C Knulst, Maria Yazdanbakhsh, Ayola A Adegnika
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb20e806408548b7b673ca2b403aea12
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spelling	oai:doaj.org-article:eb20e806408548b7b673ca2b403aea122021-11-18T08:22:40ZDifferences in innate cytokine responses between European and African children.1932-620310.1371/journal.pone.0095241https://doaj.org/article/eb20e806408548b7b673ca2b403aea122014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24743542/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Although differences in immunological responses between populations have been found in terms of vaccine efficacy, immune responses to infections and prevalence of chronic inflammatory diseases, the mechanisms responsible for these differences are not well understood. Therefore, innate cytokine responses mediated by various classes of pattern-recognition receptors including Toll-like receptors (TLR), C-type lectin receptors (CLRs) and nucleotide-binding oligomerisation domain-like receptors (NLRs) were compared between Dutch (European), semi-urban and rural Gabonese (African) children. Whole blood was stimulated for 24 hours and the pro-inflammatory tumor necrosis factor (TNF) and the anti-inflammatory/regulatory interleukin-10 (IL-10) cytokines in culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). Gabonese children had a lower pro-inflammatory response to poly(I:C) (TLR3 ligand), but a higher pro-inflammatory response to FSL-1 (TLR2/6 ligand), Pam3 (TLR2/1 ligand) and LPS (TLR4 ligand) compared to Dutch children. Anti-inflammatory responses to Pam3 were also higher in Gabonese children. Non-TLR ligands did not induce substantial cytokine production on their own. Interaction between various TLR and non-TLR receptors was further assessed, but no differences were found between the three populations. In conclusion, using a field applicable assay, significant differences were observed in cytokine responses between European and African children to TLR ligands, but not to non-TLR ligands.Lucja A LabudaSanne E de JongLynn MeursAbena S AmoahMoustapha MbowUlysse Ateba-NgoaAlwin J van der HamAndré C KnulstMaria YazdanbakhshAyola A AdegnikaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e95241 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucja A Labuda Sanne E de Jong Lynn Meurs Abena S Amoah Moustapha Mbow Ulysse Ateba-Ngoa Alwin J van der Ham André C Knulst Maria Yazdanbakhsh Ayola A Adegnika Differences in innate cytokine responses between European and African children.
description	Although differences in immunological responses between populations have been found in terms of vaccine efficacy, immune responses to infections and prevalence of chronic inflammatory diseases, the mechanisms responsible for these differences are not well understood. Therefore, innate cytokine responses mediated by various classes of pattern-recognition receptors including Toll-like receptors (TLR), C-type lectin receptors (CLRs) and nucleotide-binding oligomerisation domain-like receptors (NLRs) were compared between Dutch (European), semi-urban and rural Gabonese (African) children. Whole blood was stimulated for 24 hours and the pro-inflammatory tumor necrosis factor (TNF) and the anti-inflammatory/regulatory interleukin-10 (IL-10) cytokines in culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). Gabonese children had a lower pro-inflammatory response to poly(I:C) (TLR3 ligand), but a higher pro-inflammatory response to FSL-1 (TLR2/6 ligand), Pam3 (TLR2/1 ligand) and LPS (TLR4 ligand) compared to Dutch children. Anti-inflammatory responses to Pam3 were also higher in Gabonese children. Non-TLR ligands did not induce substantial cytokine production on their own. Interaction between various TLR and non-TLR receptors was further assessed, but no differences were found between the three populations. In conclusion, using a field applicable assay, significant differences were observed in cytokine responses between European and African children to TLR ligands, but not to non-TLR ligands.
format	article
author	Lucja A Labuda Sanne E de Jong Lynn Meurs Abena S Amoah Moustapha Mbow Ulysse Ateba-Ngoa Alwin J van der Ham André C Knulst Maria Yazdanbakhsh Ayola A Adegnika
author_facet	Lucja A Labuda Sanne E de Jong Lynn Meurs Abena S Amoah Moustapha Mbow Ulysse Ateba-Ngoa Alwin J van der Ham André C Knulst Maria Yazdanbakhsh Ayola A Adegnika
author_sort	Lucja A Labuda
title	Differences in innate cytokine responses between European and African children.
title_short	Differences in innate cytokine responses between European and African children.
title_full	Differences in innate cytokine responses between European and African children.
title_fullStr	Differences in innate cytokine responses between European and African children.
title_full_unstemmed	Differences in innate cytokine responses between European and African children.
title_sort	differences in innate cytokine responses between european and african children.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/eb20e806408548b7b673ca2b403aea12
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Differences in innate cytokine responses between European and African children.

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