Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope

The authors have recently developed molecular force microscopy (MFM) which uses fluorescence polarisation to measure cell-surface receptor force orientation. Here they show that structured illumination microscopes, which inherently use fluorescence polarisation, can be used for MFM in a turn-key man...

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Autores principales: Aaron Blanchard, J. Dale Combs, Joshua M. Brockman, Anna V. Kellner, Roxanne Glazier, Hanquan Su, Rachel L. Bender, Alisina S. Bazrafshan, Wenchun Chen, M. Edward Quach, Renhao Li, Alexa L. Mattheyses, Khalid Salaita
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/eb25c5882deb4c9a8bdcaa77f44d63b7
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spelling oai:doaj.org-article:eb25c5882deb4c9a8bdcaa77f44d63b72021-12-02T14:53:37ZTurn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope10.1038/s41467-021-24602-x2041-1723https://doaj.org/article/eb25c5882deb4c9a8bdcaa77f44d63b72021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24602-xhttps://doaj.org/toc/2041-1723The authors have recently developed molecular force microscopy (MFM) which uses fluorescence polarisation to measure cell-surface receptor force orientation. Here they show that structured illumination microscopes, which inherently use fluorescence polarisation, can be used for MFM in a turn-key manner.Aaron BlanchardJ. Dale CombsJoshua M. BrockmanAnna V. KellnerRoxanne GlazierHanquan SuRachel L. BenderAlisina S. BazrafshanWenchun ChenM. Edward QuachRenhao LiAlexa L. MattheysesKhalid SalaitaNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Aaron Blanchard
J. Dale Combs
Joshua M. Brockman
Anna V. Kellner
Roxanne Glazier
Hanquan Su
Rachel L. Bender
Alisina S. Bazrafshan
Wenchun Chen
M. Edward Quach
Renhao Li
Alexa L. Mattheyses
Khalid Salaita
Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
description The authors have recently developed molecular force microscopy (MFM) which uses fluorescence polarisation to measure cell-surface receptor force orientation. Here they show that structured illumination microscopes, which inherently use fluorescence polarisation, can be used for MFM in a turn-key manner.
format article
author Aaron Blanchard
J. Dale Combs
Joshua M. Brockman
Anna V. Kellner
Roxanne Glazier
Hanquan Su
Rachel L. Bender
Alisina S. Bazrafshan
Wenchun Chen
M. Edward Quach
Renhao Li
Alexa L. Mattheyses
Khalid Salaita
author_facet Aaron Blanchard
J. Dale Combs
Joshua M. Brockman
Anna V. Kellner
Roxanne Glazier
Hanquan Su
Rachel L. Bender
Alisina S. Bazrafshan
Wenchun Chen
M. Edward Quach
Renhao Li
Alexa L. Mattheyses
Khalid Salaita
author_sort Aaron Blanchard
title Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
title_short Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
title_full Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
title_fullStr Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
title_full_unstemmed Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
title_sort turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/eb25c5882deb4c9a8bdcaa77f44d63b7
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