Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells

Jin Sil Lee, Won Hur Department of Bioengineering and Technology, Kangwon National University, Chuncheon, South Korea Abstract: Purified fibroin protein can be obtained in large quantities from silk fibers and processed to form microscopic particles as delivery vehicles for therapeutic agents. In...

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Autores principales:	Lee JS, Hur W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Microspheres DNA delivery Transcellular transport Fibroin Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eb267f9088624831a4c78c03bd7bfde3
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spelling	oai:doaj.org-article:eb267f9088624831a4c78c03bd7bfde32021-12-02T08:20:22ZCellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells1178-2013https://doaj.org/article/eb267f9088624831a4c78c03bd7bfde32016-05-01T00:00:00Zhttps://www.dovepress.com/cellular-uptake-and-fate-of-fibroin-microspheres-loaded-with-randomly--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jin Sil Lee, Won Hur Department of Bioengineering and Technology, Kangwon National University, Chuncheon, South Korea Abstract: Purified fibroin protein can be obtained in large quantities from silk fibers and processed to form microscopic particles as delivery vehicles for therapeutic agents. In this study, we demonstrated that fibroin microspheres were taken up by 3T3 cells, localized in the nonlysosomal compartment, and secreted from the cytoplasm after medium replenishment. DNA-loaded microspheres were taken up by >95% of 3T3 cells. DNA cargo had no influence on the intracellular trafficking of microspheres, while fluorescently labeled cargo DNA was observed in the lysosomal compartment and in the microspheres. These results indicate that fibroin microspheres can travel through 3T3 cells without making any contact with the lysosomal compartments. The amount of DNA loaded in the microspheres taken up by 3T3 cells was estimated up to 831.0 pg/cell. Thus, fibroin microspheres can deliver a large amount of randomly fragmented DNA (<10 kb) into the cytoplasmic compartment of 3T3 cells. Keywords: microspheres, DNA delivery, transcellular transport, fibroin Lee JSHur WDove Medical PressarticleMicrospheresDNA deliveryTranscellular transportFibroinMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2069-2079 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microspheres DNA delivery Transcellular transport Fibroin Medicine (General) R5-920
spellingShingle	Microspheres DNA delivery Transcellular transport Fibroin Medicine (General) R5-920 Lee JS Hur W Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells
description	Jin Sil Lee, Won Hur Department of Bioengineering and Technology, Kangwon National University, Chuncheon, South Korea Abstract: Purified fibroin protein can be obtained in large quantities from silk fibers and processed to form microscopic particles as delivery vehicles for therapeutic agents. In this study, we demonstrated that fibroin microspheres were taken up by 3T3 cells, localized in the nonlysosomal compartment, and secreted from the cytoplasm after medium replenishment. DNA-loaded microspheres were taken up by >95% of 3T3 cells. DNA cargo had no influence on the intracellular trafficking of microspheres, while fluorescently labeled cargo DNA was observed in the lysosomal compartment and in the microspheres. These results indicate that fibroin microspheres can travel through 3T3 cells without making any contact with the lysosomal compartments. The amount of DNA loaded in the microspheres taken up by 3T3 cells was estimated up to 831.0 pg/cell. Thus, fibroin microspheres can deliver a large amount of randomly fragmented DNA (<10 kb) into the cytoplasmic compartment of 3T3 cells. Keywords: microspheres, DNA delivery, transcellular transport, fibroin
format	article
author	Lee JS Hur W
author_facet	Lee JS Hur W
author_sort	Lee JS
title	Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells
title_short	Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells
title_full	Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells
title_fullStr	Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells
title_full_unstemmed	Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells
title_sort	cellular uptake and fate of fibroin microspheres loaded with randomly fragmented dna in 3t3 cells
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/eb267f9088624831a4c78c03bd7bfde3
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Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3&nbsp;cells

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Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells