Phages Actively Challenge Niche Communities in Antarctic Soils

ABSTRACT By modulating the structure, diversity, and trophic outputs of microbial communities, phages play crucial roles in many biomes. In oligotrophic polar deserts, the effects of katabatic winds, constrained nutrients, and low water availability are known to limit microbial activity. Although ph...

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Autores principales:	Oliver K. I. Bezuidt, Pedro Humberto Lebre, Rian Pierneef, Carlos León-Sobrino, Evelien M. Adriaenssens, Don A. Cowan, Yves Van de Peer, Thulani P. Makhalanyane
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Antarctic soils archaea bacteria hypoliths phages viromics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/eb295f09c6b249fe924ae2ce889caa24
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spelling	oai:doaj.org-article:eb295f09c6b249fe924ae2ce889caa242021-12-02T19:47:39ZPhages Actively Challenge Niche Communities in Antarctic Soils10.1128/mSystems.00234-202379-5077https://doaj.org/article/eb295f09c6b249fe924ae2ce889caa242020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00234-20https://doaj.org/toc/2379-5077ABSTRACT By modulating the structure, diversity, and trophic outputs of microbial communities, phages play crucial roles in many biomes. In oligotrophic polar deserts, the effects of katabatic winds, constrained nutrients, and low water availability are known to limit microbial activity. Although phages may substantially govern trophic interactions in cold deserts, relatively little is known regarding the precise ecological mechanisms. Here, we provide the first evidence of widespread antiphage innate immunity in Antarctic environments using metagenomic sequence data from hypolith communities as model systems. In particular, immunity systems such as DISARM and BREX are shown to be dominant systems in these communities. Additionally, we show a direct correlation between the CRISPR-Cas adaptive immunity and the metavirome of hypolith communities, suggesting the existence of dynamic host-phage interactions. In addition to providing the first exploration of immune systems in cold deserts, our results suggest that phages actively challenge niche communities in Antarctic polar deserts. We provide evidence suggesting that the regulatory role played by phages in this system is an important determinant of bacterial host interactions in this environment. IMPORTANCE In Antarctic environments, the combination of both abiotic and biotic stressors results in simple trophic levels dominated by microbiomes. Although the past two decades have revealed substantial insights regarding the diversity and structure of microbiomes, we lack mechanistic insights regarding community interactions and how phages may affect these. By providing the first evidence of widespread antiphage innate immunity, we shed light on phage-host dynamics in Antarctic niche communities. Our analyses reveal several antiphage defense systems, including DISARM and BREX, which appear to dominate in cold desert niche communities. In contrast, our analyses revealed that genes which encode antiphage adaptive immunity were underrepresented in these communities, suggesting lower infection frequencies in cold edaphic environments. We propose that by actively challenging niche communities, phages play crucial roles in the diversification of Antarctic communities.Oliver K. I. BezuidtPedro Humberto LebreRian PierneefCarlos León-SobrinoEvelien M. AdriaenssensDon A. CowanYves Van de PeerThulani P. MakhalanyaneAmerican Society for MicrobiologyarticleAntarctic soilsarchaeabacteriahypolithsphagesviromicsMicrobiologyQR1-502ENmSystems, Vol 5, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antarctic soils archaea bacteria hypoliths phages viromics Microbiology QR1-502
spellingShingle	Antarctic soils archaea bacteria hypoliths phages viromics Microbiology QR1-502 Oliver K. I. Bezuidt Pedro Humberto Lebre Rian Pierneef Carlos León-Sobrino Evelien M. Adriaenssens Don A. Cowan Yves Van de Peer Thulani P. Makhalanyane Phages Actively Challenge Niche Communities in Antarctic Soils
description	ABSTRACT By modulating the structure, diversity, and trophic outputs of microbial communities, phages play crucial roles in many biomes. In oligotrophic polar deserts, the effects of katabatic winds, constrained nutrients, and low water availability are known to limit microbial activity. Although phages may substantially govern trophic interactions in cold deserts, relatively little is known regarding the precise ecological mechanisms. Here, we provide the first evidence of widespread antiphage innate immunity in Antarctic environments using metagenomic sequence data from hypolith communities as model systems. In particular, immunity systems such as DISARM and BREX are shown to be dominant systems in these communities. Additionally, we show a direct correlation between the CRISPR-Cas adaptive immunity and the metavirome of hypolith communities, suggesting the existence of dynamic host-phage interactions. In addition to providing the first exploration of immune systems in cold deserts, our results suggest that phages actively challenge niche communities in Antarctic polar deserts. We provide evidence suggesting that the regulatory role played by phages in this system is an important determinant of bacterial host interactions in this environment. IMPORTANCE In Antarctic environments, the combination of both abiotic and biotic stressors results in simple trophic levels dominated by microbiomes. Although the past two decades have revealed substantial insights regarding the diversity and structure of microbiomes, we lack mechanistic insights regarding community interactions and how phages may affect these. By providing the first evidence of widespread antiphage innate immunity, we shed light on phage-host dynamics in Antarctic niche communities. Our analyses reveal several antiphage defense systems, including DISARM and BREX, which appear to dominate in cold desert niche communities. In contrast, our analyses revealed that genes which encode antiphage adaptive immunity were underrepresented in these communities, suggesting lower infection frequencies in cold edaphic environments. We propose that by actively challenging niche communities, phages play crucial roles in the diversification of Antarctic communities.
format	article
author	Oliver K. I. Bezuidt Pedro Humberto Lebre Rian Pierneef Carlos León-Sobrino Evelien M. Adriaenssens Don A. Cowan Yves Van de Peer Thulani P. Makhalanyane
author_facet	Oliver K. I. Bezuidt Pedro Humberto Lebre Rian Pierneef Carlos León-Sobrino Evelien M. Adriaenssens Don A. Cowan Yves Van de Peer Thulani P. Makhalanyane
author_sort	Oliver K. I. Bezuidt
title	Phages Actively Challenge Niche Communities in Antarctic Soils
title_short	Phages Actively Challenge Niche Communities in Antarctic Soils
title_full	Phages Actively Challenge Niche Communities in Antarctic Soils
title_fullStr	Phages Actively Challenge Niche Communities in Antarctic Soils
title_full_unstemmed	Phages Actively Challenge Niche Communities in Antarctic Soils
title_sort	phages actively challenge niche communities in antarctic soils
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/eb295f09c6b249fe924ae2ce889caa24
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Phages Actively Challenge Niche Communities in Antarctic Soils

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