Layer-by-layer siRNA/poly(L-lysine) Multilayers on Polydopamine-coated Surface for Efficient Cell Adhesion and Gene Silencing

Abstract For tissue engineering applications, small interfering RNA (siRNA) is an attractive agent for controlling cellular functions and differentiation. Although polyionic condensation of nucleic acids with polycations has been widely used for gene delivery, siRNA is not strongly associated with c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cheol Am Hong, Ho Yeon Son, Yoon Sung Nam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb299038bc9645deb1e452480ca0da6a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eb299038bc9645deb1e452480ca0da6a
record_format	dspace
spelling	oai:doaj.org-article:eb299038bc9645deb1e452480ca0da6a2021-12-02T15:08:54ZLayer-by-layer siRNA/poly(L-lysine) Multilayers on Polydopamine-coated Surface for Efficient Cell Adhesion and Gene Silencing10.1038/s41598-018-25655-72045-2322https://doaj.org/article/eb299038bc9645deb1e452480ca0da6a2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25655-7https://doaj.org/toc/2045-2322Abstract For tissue engineering applications, small interfering RNA (siRNA) is an attractive agent for controlling cellular functions and differentiation. Although polyionic condensation of nucleic acids with polycations has been widely used for gene delivery, siRNA is not strongly associated with cationic carriers due to its low charge density and rigid molecular structures. The use of an excess amount of cationic carriers is often used for siRNA condensation, though they can induce severe cytotoxicity. Here we introduce the self-assembly of siRNA with mild polyelectrolytes into multilayers for efficient gene silencing during cell proliferation. The multilayers were prepared through the sequential layer-by-layer deposition of siRNA and poly-L-lysine (PLL) on a polydopamine-coated substrate. The cells, grown on the siRNA/PLL multilayers, exhibited a remarkable inhibition of the expression of target genes as compared to the use of scrambled siRNA. The gene silencing efficiency depends on the number of siRNA layers within a multilayer. This result indicates that siRNA/PLL multilayers can be potentially utilized for efficient surface-mediated siRNA delivery.Cheol Am HongHo Yeon SonYoon Sung NamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cheol Am Hong Ho Yeon Son Yoon Sung Nam Layer-by-layer siRNA/poly(L-lysine) Multilayers on Polydopamine-coated Surface for Efficient Cell Adhesion and Gene Silencing
description	Abstract For tissue engineering applications, small interfering RNA (siRNA) is an attractive agent for controlling cellular functions and differentiation. Although polyionic condensation of nucleic acids with polycations has been widely used for gene delivery, siRNA is not strongly associated with cationic carriers due to its low charge density and rigid molecular structures. The use of an excess amount of cationic carriers is often used for siRNA condensation, though they can induce severe cytotoxicity. Here we introduce the self-assembly of siRNA with mild polyelectrolytes into multilayers for efficient gene silencing during cell proliferation. The multilayers were prepared through the sequential layer-by-layer deposition of siRNA and poly-L-lysine (PLL) on a polydopamine-coated substrate. The cells, grown on the siRNA/PLL multilayers, exhibited a remarkable inhibition of the expression of target genes as compared to the use of scrambled siRNA. The gene silencing efficiency depends on the number of siRNA layers within a multilayer. This result indicates that siRNA/PLL multilayers can be potentially utilized for efficient surface-mediated siRNA delivery.
format	article
author	Cheol Am Hong Ho Yeon Son Yoon Sung Nam
author_facet	Cheol Am Hong Ho Yeon Son Yoon Sung Nam
author_sort	Cheol Am Hong
title	Layer-by-layer siRNA/poly(L-lysine) Multilayers on Polydopamine-coated Surface for Efficient Cell Adhesion and Gene Silencing
title_short	Layer-by-layer siRNA/poly(L-lysine) Multilayers on Polydopamine-coated Surface for Efficient Cell Adhesion and Gene Silencing
title_full	Layer-by-layer siRNA/poly(L-lysine) Multilayers on Polydopamine-coated Surface for Efficient Cell Adhesion and Gene Silencing
title_fullStr	Layer-by-layer siRNA/poly(L-lysine) Multilayers on Polydopamine-coated Surface for Efficient Cell Adhesion and Gene Silencing
title_full_unstemmed	Layer-by-layer siRNA/poly(L-lysine) Multilayers on Polydopamine-coated Surface for Efficient Cell Adhesion and Gene Silencing
title_sort	layer-by-layer sirna/poly(l-lysine) multilayers on polydopamine-coated surface for efficient cell adhesion and gene silencing
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/eb299038bc9645deb1e452480ca0da6a
work_keys_str_mv	AT cheolamhong layerbylayersirnapolyllysinemultilayersonpolydopaminecoatedsurfaceforefficientcelladhesionandgenesilencing AT hoyeonson layerbylayersirnapolyllysinemultilayersonpolydopaminecoatedsurfaceforefficientcelladhesionandgenesilencing AT yoonsungnam layerbylayersirnapolyllysinemultilayersonpolydopaminecoatedsurfaceforefficientcelladhesionandgenesilencing
_version_	1718387972157472768

Layer-by-layer siRNA/poly(L-lysine) Multilayers on Polydopamine-coated Surface for Efficient Cell Adhesion and Gene Silencing

Ejemplares similares