Long-term optical brain imaging in live adult fruit flies

Time-lapse imaging studies of more than a day in the fly brain have been infeasible until now. Here the authors present a laser microsurgery approach to create a permanent window in the fly cuticle to enable time-lapse imaging of neural architecture and dynamics for up to 10–50 days.

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Auteurs principaux: Cheng Huang, Jessica R. Maxey, Supriyo Sinha, Joan Savall, Yiyang Gong, Mark J. Schnitzer
Format: article
Langue:EN
Publié: Nature Portfolio 2018
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Accès en ligne:https://doaj.org/article/eb2d1b6d6bab44e19661338f459d5e9d
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Résumé:Time-lapse imaging studies of more than a day in the fly brain have been infeasible until now. Here the authors present a laser microsurgery approach to create a permanent window in the fly cuticle to enable time-lapse imaging of neural architecture and dynamics for up to 10–50 days.