The effect of pupil transmittance on axial resolution of reflection phase microscopy

Abstract A reflection phase microscope (RPM) can be equipped with the capability of depth selection by employing a gating mechanism. However, it is difficult to achieve an axial resolution close to the diffraction limit in real implementation. Here, we systematically investigated the uneven interfer...

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Autores principales:	Min Gyu Hyeon, Kwanjun Park, Taeseok Daniel Yang, Taedong Kong, Beop-Min Kim, Youngwoon Choi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb2e38b36b9f49039764e055bb71174e
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spelling	oai:doaj.org-article:eb2e38b36b9f49039764e055bb71174e2021-11-28T12:19:59ZThe effect of pupil transmittance on axial resolution of reflection phase microscopy10.1038/s41598-021-02188-02045-2322https://doaj.org/article/eb2e38b36b9f49039764e055bb71174e2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02188-0https://doaj.org/toc/2045-2322Abstract A reflection phase microscope (RPM) can be equipped with the capability of depth selection by employing a gating mechanism. However, it is difficult to achieve an axial resolution close to the diffraction limit in real implementation. Here, we systematically investigated the uneven interference contrast produced by pupil transmittance of the objective lens and found that it was the main cause of the practical limit that prevents the axial resolution from reaching its diffraction limit. Then we modulated the power of illumination light to obtain a uniform interference contrast over the entire pupil. Consequently, we could achieve an axial resolution fairly close to the diffraction limit set by the experimental conditions.Min Gyu HyeonKwanjun ParkTaeseok Daniel YangTaedong KongBeop-Min KimYoungwoon ChoiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Min Gyu Hyeon Kwanjun Park Taeseok Daniel Yang Taedong Kong Beop-Min Kim Youngwoon Choi The effect of pupil transmittance on axial resolution of reflection phase microscopy
description	Abstract A reflection phase microscope (RPM) can be equipped with the capability of depth selection by employing a gating mechanism. However, it is difficult to achieve an axial resolution close to the diffraction limit in real implementation. Here, we systematically investigated the uneven interference contrast produced by pupil transmittance of the objective lens and found that it was the main cause of the practical limit that prevents the axial resolution from reaching its diffraction limit. Then we modulated the power of illumination light to obtain a uniform interference contrast over the entire pupil. Consequently, we could achieve an axial resolution fairly close to the diffraction limit set by the experimental conditions.
format	article
author	Min Gyu Hyeon Kwanjun Park Taeseok Daniel Yang Taedong Kong Beop-Min Kim Youngwoon Choi
author_facet	Min Gyu Hyeon Kwanjun Park Taeseok Daniel Yang Taedong Kong Beop-Min Kim Youngwoon Choi
author_sort	Min Gyu Hyeon
title	The effect of pupil transmittance on axial resolution of reflection phase microscopy
title_short	The effect of pupil transmittance on axial resolution of reflection phase microscopy
title_full	The effect of pupil transmittance on axial resolution of reflection phase microscopy
title_fullStr	The effect of pupil transmittance on axial resolution of reflection phase microscopy
title_full_unstemmed	The effect of pupil transmittance on axial resolution of reflection phase microscopy
title_sort	effect of pupil transmittance on axial resolution of reflection phase microscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eb2e38b36b9f49039764e055bb71174e
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The effect of pupil transmittance on axial resolution of reflection phase microscopy

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