Natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.

Accumulation of filamentous actin (F-actin) at the immunological synapse (IS) is a prerequisite for the cytotoxic function of natural killer (NK) cells. Subsequent to reorganization of the actin network, lytic granules polarize to the IS where their contents are secreted directly toward a target cel...

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Autores principales:	Gregory D Rak, Emily M Mace, Pinaki P Banerjee, Tatyana Svitkina, Jordan S Orange
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/eb2e3b7659274a04bdb6c15af54a7b2a
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spelling	oai:doaj.org-article:eb2e3b7659274a04bdb6c15af54a7b2a2021-11-04T05:33:00ZNatural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.1544-91731545-788510.1371/journal.pbio.1001151https://doaj.org/article/eb2e3b7659274a04bdb6c15af54a7b2a2011-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21931536/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Accumulation of filamentous actin (F-actin) at the immunological synapse (IS) is a prerequisite for the cytotoxic function of natural killer (NK) cells. Subsequent to reorganization of the actin network, lytic granules polarize to the IS where their contents are secreted directly toward a target cell, providing critical access to host defense. There has been limited investigation into the relationship between the actin network and degranulation. Thus, we have evaluated the actin network and secretion using microscopy techniques that provide unprecedented resolution and/or functional insight. We show that the actin network extends throughout the IS and that degranulation occurs in areas where there is actin, albeit in sub-micron relatively hypodense regions. Therefore we propose that granules reach the plasma membrane in clearances in the network that are appropriately sized to minimally accommodate a granule and allow it to interact with the filaments. Our data support a model whereby lytic granules and the actin network are intimately associated during the secretion process and broadly suggest a mechanism for the secretion of large organelles in the context of a cortical actin barrier.Gregory D RakEmily M MacePinaki P BanerjeeTatyana SvitkinaJordan S OrangePublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 9, Iss 9, p e1001151 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Gregory D Rak Emily M Mace Pinaki P Banerjee Tatyana Svitkina Jordan S Orange Natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.
description	Accumulation of filamentous actin (F-actin) at the immunological synapse (IS) is a prerequisite for the cytotoxic function of natural killer (NK) cells. Subsequent to reorganization of the actin network, lytic granules polarize to the IS where their contents are secreted directly toward a target cell, providing critical access to host defense. There has been limited investigation into the relationship between the actin network and degranulation. Thus, we have evaluated the actin network and secretion using microscopy techniques that provide unprecedented resolution and/or functional insight. We show that the actin network extends throughout the IS and that degranulation occurs in areas where there is actin, albeit in sub-micron relatively hypodense regions. Therefore we propose that granules reach the plasma membrane in clearances in the network that are appropriately sized to minimally accommodate a granule and allow it to interact with the filaments. Our data support a model whereby lytic granules and the actin network are intimately associated during the secretion process and broadly suggest a mechanism for the secretion of large organelles in the context of a cortical actin barrier.
format	article
author	Gregory D Rak Emily M Mace Pinaki P Banerjee Tatyana Svitkina Jordan S Orange
author_facet	Gregory D Rak Emily M Mace Pinaki P Banerjee Tatyana Svitkina Jordan S Orange
author_sort	Gregory D Rak
title	Natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.
title_short	Natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.
title_full	Natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.
title_fullStr	Natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.
title_full_unstemmed	Natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.
title_sort	natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/eb2e3b7659274a04bdb6c15af54a7b2a
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Natural killer cell lytic granule secretion occurs through a pervasive actin network at the immune synapse.

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