The Serum from Patients with Secondary Frozen Shoulder Following Rotator Cuff Repair Induces Shoulder Capsule Fibrosis and Promotes Macrophage Polarization and Fibroblast Activation

Yaying Sun,* Jinrong Lin,* Zhiwen Luo,* Yuhan Zhang, Jiwu Chen Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jiwu ChenDepartment of Sports Medicine, Huashan Hospital, Fud...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sun Y, Lin J, Luo Z, Zhang Y, Chen J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	rotator cuff secondary frozen shoulder serum macrophage fibroblast Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/eb3384372b634c0fbae899d1cfbf02ba
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eb3384372b634c0fbae899d1cfbf02ba
record_format	dspace
spelling	oai:doaj.org-article:eb3384372b634c0fbae899d1cfbf02ba2021-12-02T11:41:55ZThe Serum from Patients with Secondary Frozen Shoulder Following Rotator Cuff Repair Induces Shoulder Capsule Fibrosis and Promotes Macrophage Polarization and Fibroblast Activation1178-7031https://doaj.org/article/eb3384372b634c0fbae899d1cfbf02ba2021-03-01T00:00:00Zhttps://www.dovepress.com/the-serum-from-patients-with-secondary-frozen-shoulder-following-rotat-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Yaying Sun,* Jinrong Lin,* Zhiwen Luo,* Yuhan Zhang, Jiwu Chen Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jiwu ChenDepartment of Sports Medicine, Huashan Hospital, Fudan University, 12# Middle Wulumuqi Road, Jing’an District, Shanghai, 200040, People’s Republic of ChinaFax +86 21 52888255Email jeevechen@gmail.comPurpose: Disorders with systematic inflammation were prognostic for secondary frozen shoulder (sFS) following rotator cuff repair (RCR); however, how systematic inflammation affects sFS remains unclear. The aim of this study was to observe the effect of pre-operative serum from patients with sFS and the serum from those without on shoulder capsule in mice, and on macrophages and fibroblasts in vitro.Methods: Serum samples of a consecutive cohort of patients for RCR were collected pre-operatively. Three months after RCR, patients who developed sFS (Group S) were identified. Serum samples from gender- and age-matched controls without sFS (group NS) were also picked out. Firstly, the effect of serum on shoulder capsule fibrosis was observed histologically and biomechanically in a mouse model of RCR. Secondly, the roles of the serum on macrophage polarization and fibroblast activation were investigated, and the potentially involved signaling pathways were identified. Finally, inflammation and fibrosis-related cytokines in serum were quantified.Results: In our cohort, all patients had free pre-operative shoulder range of motion. Seven patients developed sFS at 3 months after surgery. Seven matched patients without sFS were selected as control. The inter-group difference of basic characteristics was not significant. Compared to the serum of group NS, the serum of group S significantly induced hypercellularity, capsular thickening, and range of motion deficiency in mice shoulders after RCR. Compared to the serum of group NS, samples of group S significantly promoted M2 polarization of THP-1 human macrophages and the activation of human capsule-derived fibroblasts. Meanwhile, Smad3 and p-Smad3 in macrophages and fibroblasts were significantly up-regulated. On the other hand, levels of inflammation and fibrosis-related cytokines were not significantly different between serum in group S and group NS.Conclusion: Although all patients in this cohort had free range of motion pre-operatively, the pre-operative serum from patients with sFS at 3 months after RCR could act as a trigger of shoulder capsule fibrosis post-operatively. This effect may be related to its promotion on macrophage polarization to M2 phenotype and fibroblast activation.Keywords: rotator cuff, secondary frozen shoulder, serum, macrophage, fibroblastSun YLin JLuo ZZhang YChen JDove Medical Pressarticlerotator cuffsecondary frozen shoulderserummacrophagefibroblastPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 1055-1068 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rotator cuff secondary frozen shoulder serum macrophage fibroblast Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	rotator cuff secondary frozen shoulder serum macrophage fibroblast Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Sun Y Lin J Luo Z Zhang Y Chen J The Serum from Patients with Secondary Frozen Shoulder Following Rotator Cuff Repair Induces Shoulder Capsule Fibrosis and Promotes Macrophage Polarization and Fibroblast Activation
description	Yaying Sun,* Jinrong Lin,* Zhiwen Luo,* Yuhan Zhang, Jiwu Chen Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jiwu ChenDepartment of Sports Medicine, Huashan Hospital, Fudan University, 12# Middle Wulumuqi Road, Jing’an District, Shanghai, 200040, People’s Republic of ChinaFax +86 21 52888255Email jeevechen@gmail.comPurpose: Disorders with systematic inflammation were prognostic for secondary frozen shoulder (sFS) following rotator cuff repair (RCR); however, how systematic inflammation affects sFS remains unclear. The aim of this study was to observe the effect of pre-operative serum from patients with sFS and the serum from those without on shoulder capsule in mice, and on macrophages and fibroblasts in vitro.Methods: Serum samples of a consecutive cohort of patients for RCR were collected pre-operatively. Three months after RCR, patients who developed sFS (Group S) were identified. Serum samples from gender- and age-matched controls without sFS (group NS) were also picked out. Firstly, the effect of serum on shoulder capsule fibrosis was observed histologically and biomechanically in a mouse model of RCR. Secondly, the roles of the serum on macrophage polarization and fibroblast activation were investigated, and the potentially involved signaling pathways were identified. Finally, inflammation and fibrosis-related cytokines in serum were quantified.Results: In our cohort, all patients had free pre-operative shoulder range of motion. Seven patients developed sFS at 3 months after surgery. Seven matched patients without sFS were selected as control. The inter-group difference of basic characteristics was not significant. Compared to the serum of group NS, the serum of group S significantly induced hypercellularity, capsular thickening, and range of motion deficiency in mice shoulders after RCR. Compared to the serum of group NS, samples of group S significantly promoted M2 polarization of THP-1 human macrophages and the activation of human capsule-derived fibroblasts. Meanwhile, Smad3 and p-Smad3 in macrophages and fibroblasts were significantly up-regulated. On the other hand, levels of inflammation and fibrosis-related cytokines were not significantly different between serum in group S and group NS.Conclusion: Although all patients in this cohort had free range of motion pre-operatively, the pre-operative serum from patients with sFS at 3 months after RCR could act as a trigger of shoulder capsule fibrosis post-operatively. This effect may be related to its promotion on macrophage polarization to M2 phenotype and fibroblast activation.Keywords: rotator cuff, secondary frozen shoulder, serum, macrophage, fibroblast
format	article
author	Sun Y Lin J Luo Z Zhang Y Chen J
author_facet	Sun Y Lin J Luo Z Zhang Y Chen J
author_sort	Sun Y
title	The Serum from Patients with Secondary Frozen Shoulder Following Rotator Cuff Repair Induces Shoulder Capsule Fibrosis and Promotes Macrophage Polarization and Fibroblast Activation
title_short	The Serum from Patients with Secondary Frozen Shoulder Following Rotator Cuff Repair Induces Shoulder Capsule Fibrosis and Promotes Macrophage Polarization and Fibroblast Activation
title_full	The Serum from Patients with Secondary Frozen Shoulder Following Rotator Cuff Repair Induces Shoulder Capsule Fibrosis and Promotes Macrophage Polarization and Fibroblast Activation
title_fullStr	The Serum from Patients with Secondary Frozen Shoulder Following Rotator Cuff Repair Induces Shoulder Capsule Fibrosis and Promotes Macrophage Polarization and Fibroblast Activation
title_full_unstemmed	The Serum from Patients with Secondary Frozen Shoulder Following Rotator Cuff Repair Induces Shoulder Capsule Fibrosis and Promotes Macrophage Polarization and Fibroblast Activation
title_sort	serum from patients with secondary frozen shoulder following rotator cuff repair induces shoulder capsule fibrosis and promotes macrophage polarization and fibroblast activation
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/eb3384372b634c0fbae899d1cfbf02ba
work_keys_str_mv	AT suny theserumfrompatientswithsecondaryfrozenshoulderfollowingrotatorcuffrepairinducesshouldercapsulefibrosisandpromotesmacrophagepolarizationandfibroblastactivation AT linj theserumfrompatientswithsecondaryfrozenshoulderfollowingrotatorcuffrepairinducesshouldercapsulefibrosisandpromotesmacrophagepolarizationandfibroblastactivation AT luoz theserumfrompatientswithsecondaryfrozenshoulderfollowingrotatorcuffrepairinducesshouldercapsulefibrosisandpromotesmacrophagepolarizationandfibroblastactivation AT zhangy theserumfrompatientswithsecondaryfrozenshoulderfollowingrotatorcuffrepairinducesshouldercapsulefibrosisandpromotesmacrophagepolarizationandfibroblastactivation AT chenj theserumfrompatientswithsecondaryfrozenshoulderfollowingrotatorcuffrepairinducesshouldercapsulefibrosisandpromotesmacrophagepolarizationandfibroblastactivation AT suny serumfrompatientswithsecondaryfrozenshoulderfollowingrotatorcuffrepairinducesshouldercapsulefibrosisandpromotesmacrophagepolarizationandfibroblastactivation AT linj serumfrompatientswithsecondaryfrozenshoulderfollowingrotatorcuffrepairinducesshouldercapsulefibrosisandpromotesmacrophagepolarizationandfibroblastactivation AT luoz serumfrompatientswithsecondaryfrozenshoulderfollowingrotatorcuffrepairinducesshouldercapsulefibrosisandpromotesmacrophagepolarizationandfibroblastactivation AT zhangy serumfrompatientswithsecondaryfrozenshoulderfollowingrotatorcuffrepairinducesshouldercapsulefibrosisandpromotesmacrophagepolarizationandfibroblastactivation AT chenj serumfrompatientswithsecondaryfrozenshoulderfollowingrotatorcuffrepairinducesshouldercapsulefibrosisandpromotesmacrophagepolarizationandfibroblastactivation
_version_	1718395434658955264

The Serum from Patients with Secondary Frozen Shoulder Following Rotator Cuff Repair Induces Shoulder Capsule Fibrosis and Promotes Macrophage Polarization and Fibroblast Activation

Ejemplares similares