Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation

In the past two decades, testicular tissue grafting and xenografting have been well established, with the production of fertilization-competent sperm in some studies. However, few studies have been carried out to observe the development of grafted prepubertal testicular tissue of rats and compare th...

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Autores principales:	Yu-Bo Ma, Ming Gao, Tong-Dian Zhang, Tie Chong, He-Cheng Li, Zi-Ming Wang, Lian-Dong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/eb3854e359aa4ca9ac0e18263d687cc0
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spelling	oai:doaj.org-article:eb3854e359aa4ca9ac0e18263d687cc02021-11-29T00:56:49ZOxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation1942-099410.1155/2021/1699990https://doaj.org/article/eb3854e359aa4ca9ac0e18263d687cc02021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/1699990https://doaj.org/toc/1942-0994In the past two decades, testicular tissue grafting and xenografting have been well established, with the production of fertilization-competent sperm in some studies. However, few studies have been carried out to observe the development of grafted prepubertal testicular tissue of rats and compare the biological differences between in situ testis and grafted testis. In this study, we established the prepubertal testicular tissue xenografting model using a 22-day-old rat and evaluated certain parameters, including testicular histology, testosterone production, and ultrastructure of the grafted testes. We also assessed gene expression of cell proliferation markers, testicular cell markers, and antioxidative defense system. Our results showed that 47 days after transplantation, intratesticular testosterone concentration was not significantly altered; however, cell proliferation, spermatogenesis, and Sertoli cell markers in the transplanted testes were significantly disrupted compared with the control group, accompanied by aggravated apoptosis and oxidative damage. Moreover, the transplanted testes showed smaller tubular diameter and disrupted spermatogenic epithelium with apparent vacuoles, distorted and degenerated germ cells with obscure nuclear margin, and no spermatids in the center of the tubules. Although testis xenografting has been extensively tested and attained great achievement in other species, the prepubertal rat testicular tissue xenografting to immunodeficient mice exhibited obvious spermatogenesis arrest and oxidative damage. The protocol still needs further optimization, and there are still some unknown factors in prepubertal rat testes transplantation.Yu-Bo MaMing GaoTong-Dian ZhangTie ChongHe-Cheng LiZi-Ming WangLian-Dong ZhangHindawi LimitedarticleCytologyQH573-671ENOxidative Medicine and Cellular Longevity, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Yu-Bo Ma Ming Gao Tong-Dian Zhang Tie Chong He-Cheng Li Zi-Ming Wang Lian-Dong Zhang Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation
description	In the past two decades, testicular tissue grafting and xenografting have been well established, with the production of fertilization-competent sperm in some studies. However, few studies have been carried out to observe the development of grafted prepubertal testicular tissue of rats and compare the biological differences between in situ testis and grafted testis. In this study, we established the prepubertal testicular tissue xenografting model using a 22-day-old rat and evaluated certain parameters, including testicular histology, testosterone production, and ultrastructure of the grafted testes. We also assessed gene expression of cell proliferation markers, testicular cell markers, and antioxidative defense system. Our results showed that 47 days after transplantation, intratesticular testosterone concentration was not significantly altered; however, cell proliferation, spermatogenesis, and Sertoli cell markers in the transplanted testes were significantly disrupted compared with the control group, accompanied by aggravated apoptosis and oxidative damage. Moreover, the transplanted testes showed smaller tubular diameter and disrupted spermatogenic epithelium with apparent vacuoles, distorted and degenerated germ cells with obscure nuclear margin, and no spermatids in the center of the tubules. Although testis xenografting has been extensively tested and attained great achievement in other species, the prepubertal rat testicular tissue xenografting to immunodeficient mice exhibited obvious spermatogenesis arrest and oxidative damage. The protocol still needs further optimization, and there are still some unknown factors in prepubertal rat testes transplantation.
format	article
author	Yu-Bo Ma Ming Gao Tong-Dian Zhang Tie Chong He-Cheng Li Zi-Ming Wang Lian-Dong Zhang
author_facet	Yu-Bo Ma Ming Gao Tong-Dian Zhang Tie Chong He-Cheng Li Zi-Ming Wang Lian-Dong Zhang
author_sort	Yu-Bo Ma
title	Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation
title_short	Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation
title_full	Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation
title_fullStr	Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation
title_full_unstemmed	Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation
title_sort	oxidative stress disrupted prepubertal rat testicular development after xenotransplantation
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/eb3854e359aa4ca9ac0e18263d687cc0
work_keys_str_mv	AT yuboma oxidativestressdisruptedprepubertalrattesticulardevelopmentafterxenotransplantation AT minggao oxidativestressdisruptedprepubertalrattesticulardevelopmentafterxenotransplantation AT tongdianzhang oxidativestressdisruptedprepubertalrattesticulardevelopmentafterxenotransplantation AT tiechong oxidativestressdisruptedprepubertalrattesticulardevelopmentafterxenotransplantation AT hechengli oxidativestressdisruptedprepubertalrattesticulardevelopmentafterxenotransplantation AT zimingwang oxidativestressdisruptedprepubertalrattesticulardevelopmentafterxenotransplantation AT liandongzhang oxidativestressdisruptedprepubertalrattesticulardevelopmentafterxenotransplantation
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Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation

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