Histamine H2 Receptor-Mediated Suppression of Intestinal Inflammation by Probiotic <named-content content-type="genus-species">Lactobacillus reuteri</named-content>

ABSTRACT Probiotics and commensal intestinal microbes suppress mammalian cytokine production and intestinal inflammation in various experimental model systems. Limited information exists regarding potential mechanisms of probiotic-mediated immunomodulation in vivo. In this report, we demonstrate tha...

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Autores principales:	Chunxu Gao, Angela Major, David Rendon, Monica Lugo, Vanessa Jackson, Zhongcheng Shi, Yuko Mori-Akiyama, James Versalovic
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/eb3b3982c86c4934b19bccfa57f04b6e
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spelling	oai:doaj.org-article:eb3b3982c86c4934b19bccfa57f04b6e2021-11-15T15:41:24ZHistamine H2 Receptor-Mediated Suppression of Intestinal Inflammation by Probiotic <named-content content-type="genus-species">Lactobacillus reuteri</named-content>10.1128/mBio.01358-152150-7511https://doaj.org/article/eb3b3982c86c4934b19bccfa57f04b6e2015-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01358-15https://doaj.org/toc/2150-7511ABSTRACT Probiotics and commensal intestinal microbes suppress mammalian cytokine production and intestinal inflammation in various experimental model systems. Limited information exists regarding potential mechanisms of probiotic-mediated immunomodulation in vivo. In this report, we demonstrate that specific probiotic strains of Lactobacillus reuteri suppress intestinal inflammation in a trinitrobenzene sulfonic acid (TNBS)-induced mouse colitis model. Only strains that possess the hdc gene cluster, including the histidine decarboxylase and histidine-histamine antiporter genes, can suppress colitis and mucosal cytokine (interleukin-6 [IL-6] and IL-1β in the colon) gene expression. Suppression of acute colitis in mice was documented by diminished weight loss, colonic injury, serum amyloid A (SAA) protein concentrations, and reduced uptake of [18F]fluorodeoxyglucose ([18F]FDG) in the colon by positron emission tomography (PET). The ability of probiotic L. reuteri to suppress colitis depends on the presence of a bacterial histidine decarboxylase gene(s) in the intestinal microbiome, consumption of a histidine-containing diet, and signaling via the histamine H2 receptor (H2R). Collectively, luminal conversion of l-histidine to histamine by hdc+ L. reuteri activates H2R, and H2R signaling results in suppression of acute inflammation within the mouse colon.IMPORTANCE Probiotics are microorganisms that when administered in adequate amounts confer beneficial effects on the host. Supplementation with probiotic strains was shown to suppress intestinal inflammation in patients with inflammatory bowel disease and in rodent colitis models. However, the mechanisms of probiosis are not clear. Our current studies suggest that supplementation with hdc+ L. reuteri, which can convert l-histidine to histamine in the gut, resulted in suppression of colonic inflammation. These findings link luminal conversion of dietary components (amino acid metabolism) by gut microbes and probiotic-mediated suppression of colonic inflammation. The effective combination of diet, gut bacteria, and host receptor-mediated signaling may result in opportunities for therapeutic microbiology and provide clues for discovery and development of next-generation probiotics.Chunxu GaoAngela MajorDavid RendonMonica LugoVanessa JacksonZhongcheng ShiYuko Mori-AkiyamaJames VersalovicAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 6 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Chunxu Gao Angela Major David Rendon Monica Lugo Vanessa Jackson Zhongcheng Shi Yuko Mori-Akiyama James Versalovic Histamine H2 Receptor-Mediated Suppression of Intestinal Inflammation by Probiotic <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
description	ABSTRACT Probiotics and commensal intestinal microbes suppress mammalian cytokine production and intestinal inflammation in various experimental model systems. Limited information exists regarding potential mechanisms of probiotic-mediated immunomodulation in vivo. In this report, we demonstrate that specific probiotic strains of Lactobacillus reuteri suppress intestinal inflammation in a trinitrobenzene sulfonic acid (TNBS)-induced mouse colitis model. Only strains that possess the hdc gene cluster, including the histidine decarboxylase and histidine-histamine antiporter genes, can suppress colitis and mucosal cytokine (interleukin-6 [IL-6] and IL-1β in the colon) gene expression. Suppression of acute colitis in mice was documented by diminished weight loss, colonic injury, serum amyloid A (SAA) protein concentrations, and reduced uptake of [18F]fluorodeoxyglucose ([18F]FDG) in the colon by positron emission tomography (PET). The ability of probiotic L. reuteri to suppress colitis depends on the presence of a bacterial histidine decarboxylase gene(s) in the intestinal microbiome, consumption of a histidine-containing diet, and signaling via the histamine H2 receptor (H2R). Collectively, luminal conversion of l-histidine to histamine by hdc+ L. reuteri activates H2R, and H2R signaling results in suppression of acute inflammation within the mouse colon.IMPORTANCE Probiotics are microorganisms that when administered in adequate amounts confer beneficial effects on the host. Supplementation with probiotic strains was shown to suppress intestinal inflammation in patients with inflammatory bowel disease and in rodent colitis models. However, the mechanisms of probiosis are not clear. Our current studies suggest that supplementation with hdc+ L. reuteri, which can convert l-histidine to histamine in the gut, resulted in suppression of colonic inflammation. These findings link luminal conversion of dietary components (amino acid metabolism) by gut microbes and probiotic-mediated suppression of colonic inflammation. The effective combination of diet, gut bacteria, and host receptor-mediated signaling may result in opportunities for therapeutic microbiology and provide clues for discovery and development of next-generation probiotics.
format	article
author	Chunxu Gao Angela Major David Rendon Monica Lugo Vanessa Jackson Zhongcheng Shi Yuko Mori-Akiyama James Versalovic
author_facet	Chunxu Gao Angela Major David Rendon Monica Lugo Vanessa Jackson Zhongcheng Shi Yuko Mori-Akiyama James Versalovic
author_sort	Chunxu Gao
title	Histamine H2 Receptor-Mediated Suppression of Intestinal Inflammation by Probiotic <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
title_short	Histamine H2 Receptor-Mediated Suppression of Intestinal Inflammation by Probiotic <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
title_full	Histamine H2 Receptor-Mediated Suppression of Intestinal Inflammation by Probiotic <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
title_fullStr	Histamine H2 Receptor-Mediated Suppression of Intestinal Inflammation by Probiotic <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
title_full_unstemmed	Histamine H2 Receptor-Mediated Suppression of Intestinal Inflammation by Probiotic <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
title_sort	histamine h2 receptor-mediated suppression of intestinal inflammation by probiotic <named-content content-type="genus-species">lactobacillus reuteri</named-content>
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/eb3b3982c86c4934b19bccfa57f04b6e
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Histamine H2 Receptor-Mediated Suppression of Intestinal Inflammation by Probiotic <named-content content-type="genus-species">Lactobacillus reuteri</named-content>

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