Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing

Yan Wang1, Chen-lu Zhang2, Qun Zhang1, Ping Li1,31School of Life Sciences and Technology, Tongji University, Shanghai, People&rsquo;s Republic of China; 2College of Life Sciences, Nanjing Agricultural University, Nanjing, People&rsquo;s Republic of China; 3Shanghai Key Laboratory of...

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Autores principales:	Li P, Wang Y, Zhang CL, Zhang Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eb3d591a1abc4cc98432f0b5dce798b2
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spelling	oai:doaj.org-article:eb3d591a1abc4cc98432f0b5dce798b22021-12-02T07:14:17ZComposite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing1176-91141178-2013https://doaj.org/article/eb3d591a1abc4cc98432f0b5dce798b22011-04-01T00:00:00Zhttp://www.dovepress.com/composite-electrospun-nanomembranes-of-fish-scale-collagen-peptideschi-a6995https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yan Wang1, Chen-lu Zhang2, Qun Zhang1, Ping Li1,31School of Life Sciences and Technology, Tongji University, Shanghai, People&rsquo;s Republic of China; 2College of Life Sciences, Nanjing Agricultural University, Nanjing, People&rsquo;s Republic of China; 3Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, People&rsquo;s Republic of ChinaPurpose: The objective of the present investigation was to evaluate the antibacterial properties and the biocompatibility of composite electrospun nanofibrous membranes (NFMs) with low-molecular-weight fish scale collagen peptides (FSCP) and chito-oligosaccharide (COS), to determine their potential for use as wound dressings.Methods: Low-molecular-weight FSCP were combined with COS to prepare nanofibers by electrospinning, and polyvinyl alcohol (PVA) was used for enhancing fiber-forming ability. Transmission electron microscope and scanning electron microscope methods were used to observe bacterial adhesion and the bacterial cell membrane. Fibroblast cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Results: The best FSCP/COS mass ratio for electrospinning was 2:1, and the nanofibers had small dimensions ranging from 50 to 100 nm. The NFM showed good antibacterial activities against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The antibacterial activity against S. aureus was higher than against E. coli. The pili and adhesive fimbriae of E. coli promoted bacterial adhesion to the NFM surfaces, and S. aureus biofilms aided S. aureus adhesion on the surface of NFMs. Damage to the bacterial cell membrane indicates that the NFMs could lead to the release of intracellular materials, particularly with S. aureus. In addition, FSCP/COS NFM rapidly increased the permeability of the outer membranes of E. coli. The electrospun NFM with FSCP and COS had good biocompatibility in vitro and supported proliferation of human skin fibroblasts.Conclusion: FSCP are superior to mammalian collagen, and have feasibility and potency for wound dressings. FSCP/COS NFMs had good anti-bactericidal activity that improved with increased COS, and showed good biocompatibility in vitro and supported the proliferation of fibroblasts.Keywords: composite electrospun nanomembranes, fish scale collagen peptides, bacterial adhesion, bacterial cell membrane, wound dressing Li PWang YZhang CLZhang QDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 667-676 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Li P Wang Y Zhang CL Zhang Q Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing
description	Yan Wang1, Chen-lu Zhang2, Qun Zhang1, Ping Li1,31School of Life Sciences and Technology, Tongji University, Shanghai, People&rsquo;s Republic of China; 2College of Life Sciences, Nanjing Agricultural University, Nanjing, People&rsquo;s Republic of China; 3Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, People&rsquo;s Republic of ChinaPurpose: The objective of the present investigation was to evaluate the antibacterial properties and the biocompatibility of composite electrospun nanofibrous membranes (NFMs) with low-molecular-weight fish scale collagen peptides (FSCP) and chito-oligosaccharide (COS), to determine their potential for use as wound dressings.Methods: Low-molecular-weight FSCP were combined with COS to prepare nanofibers by electrospinning, and polyvinyl alcohol (PVA) was used for enhancing fiber-forming ability. Transmission electron microscope and scanning electron microscope methods were used to observe bacterial adhesion and the bacterial cell membrane. Fibroblast cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Results: The best FSCP/COS mass ratio for electrospinning was 2:1, and the nanofibers had small dimensions ranging from 50 to 100 nm. The NFM showed good antibacterial activities against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The antibacterial activity against S. aureus was higher than against E. coli. The pili and adhesive fimbriae of E. coli promoted bacterial adhesion to the NFM surfaces, and S. aureus biofilms aided S. aureus adhesion on the surface of NFMs. Damage to the bacterial cell membrane indicates that the NFMs could lead to the release of intracellular materials, particularly with S. aureus. In addition, FSCP/COS NFM rapidly increased the permeability of the outer membranes of E. coli. The electrospun NFM with FSCP and COS had good biocompatibility in vitro and supported proliferation of human skin fibroblasts.Conclusion: FSCP are superior to mammalian collagen, and have feasibility and potency for wound dressings. FSCP/COS NFMs had good anti-bactericidal activity that improved with increased COS, and showed good biocompatibility in vitro and supported the proliferation of fibroblasts.Keywords: composite electrospun nanomembranes, fish scale collagen peptides, bacterial adhesion, bacterial cell membrane, wound dressing
format	article
author	Li P Wang Y Zhang CL Zhang Q
author_facet	Li P Wang Y Zhang CL Zhang Q
author_sort	Li P
title	Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing
title_short	Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing
title_full	Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing
title_fullStr	Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing
title_full_unstemmed	Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing
title_sort	composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/eb3d591a1abc4cc98432f0b5dce798b2
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Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing

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