Quantitative theory for the diffusive dynamics of liquid condensates

Key processes of biological condensates are diffusion and material exchange with their environment. Experimentally, diffusive dynamics are typically probed via fluorescent labels. However, to date, a physics-based, quantitative framework for the dynamics of labeled condensate components is lacking....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lars Hubatsch, Louise M Jawerth, Celina Love, Jonathan Bauermann, TY Dora Tang, Stefano Bo, Anthony A Hyman, Christoph A Weber
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	phase separation FRAP quantitative modelling Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/eb40ee1913f644cab05a643ca5e29dae
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eb40ee1913f644cab05a643ca5e29dae
record_format	dspace
spelling	oai:doaj.org-article:eb40ee1913f644cab05a643ca5e29dae2021-11-10T11:50:57ZQuantitative theory for the diffusive dynamics of liquid condensates10.7554/eLife.686202050-084Xe68620https://doaj.org/article/eb40ee1913f644cab05a643ca5e29dae2021-10-01T00:00:00Zhttps://elifesciences.org/articles/68620https://doaj.org/toc/2050-084XKey processes of biological condensates are diffusion and material exchange with their environment. Experimentally, diffusive dynamics are typically probed via fluorescent labels. However, to date, a physics-based, quantitative framework for the dynamics of labeled condensate components is lacking. Here, we derive the corresponding dynamic equations, building on the physics of phase separation, and quantitatively validate the related framework via experiments. We show that by using our framework, we can precisely determine diffusion coefficients inside liquid condensates via a spatio-temporal analysis of fluorescence recovery after photobleaching (FRAP) experiments. We showcase the accuracy and precision of our approach by considering space- and time-resolved data of protein condensates and two different polyelectrolyte-coacervate systems. Interestingly, our theory can also be used to determine a relationship between the diffusion coefficient in the dilute phase and the partition coefficient, without relying on fluorescence measurements in the dilute phase. This enables us to investigate the effect of salt addition on partitioning and bypasses recently described quenching artifacts in the dense phase. Our approach opens new avenues for theoretically describing molecule dynamics in condensates, measuring concentrations based on the dynamics of fluorescence intensities, and quantifying rates of biochemical reactions in liquid condensates.Lars HubatschLouise M JawerthCelina LoveJonathan BauermannTY Dora TangStefano BoAnthony A HymanChristoph A WebereLife Sciences Publications Ltdarticlephase separationFRAPquantitative modellingMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	phase separation FRAP quantitative modelling Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	phase separation FRAP quantitative modelling Medicine R Science Q Biology (General) QH301-705.5 Lars Hubatsch Louise M Jawerth Celina Love Jonathan Bauermann TY Dora Tang Stefano Bo Anthony A Hyman Christoph A Weber Quantitative theory for the diffusive dynamics of liquid condensates
description	Key processes of biological condensates are diffusion and material exchange with their environment. Experimentally, diffusive dynamics are typically probed via fluorescent labels. However, to date, a physics-based, quantitative framework for the dynamics of labeled condensate components is lacking. Here, we derive the corresponding dynamic equations, building on the physics of phase separation, and quantitatively validate the related framework via experiments. We show that by using our framework, we can precisely determine diffusion coefficients inside liquid condensates via a spatio-temporal analysis of fluorescence recovery after photobleaching (FRAP) experiments. We showcase the accuracy and precision of our approach by considering space- and time-resolved data of protein condensates and two different polyelectrolyte-coacervate systems. Interestingly, our theory can also be used to determine a relationship between the diffusion coefficient in the dilute phase and the partition coefficient, without relying on fluorescence measurements in the dilute phase. This enables us to investigate the effect of salt addition on partitioning and bypasses recently described quenching artifacts in the dense phase. Our approach opens new avenues for theoretically describing molecule dynamics in condensates, measuring concentrations based on the dynamics of fluorescence intensities, and quantifying rates of biochemical reactions in liquid condensates.
format	article
author	Lars Hubatsch Louise M Jawerth Celina Love Jonathan Bauermann TY Dora Tang Stefano Bo Anthony A Hyman Christoph A Weber
author_facet	Lars Hubatsch Louise M Jawerth Celina Love Jonathan Bauermann TY Dora Tang Stefano Bo Anthony A Hyman Christoph A Weber
author_sort	Lars Hubatsch
title	Quantitative theory for the diffusive dynamics of liquid condensates
title_short	Quantitative theory for the diffusive dynamics of liquid condensates
title_full	Quantitative theory for the diffusive dynamics of liquid condensates
title_fullStr	Quantitative theory for the diffusive dynamics of liquid condensates
title_full_unstemmed	Quantitative theory for the diffusive dynamics of liquid condensates
title_sort	quantitative theory for the diffusive dynamics of liquid condensates
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/eb40ee1913f644cab05a643ca5e29dae
work_keys_str_mv	AT larshubatsch quantitativetheoryforthediffusivedynamicsofliquidcondensates AT louisemjawerth quantitativetheoryforthediffusivedynamicsofliquidcondensates AT celinalove quantitativetheoryforthediffusivedynamicsofliquidcondensates AT jonathanbauermann quantitativetheoryforthediffusivedynamicsofliquidcondensates AT tydoratang quantitativetheoryforthediffusivedynamicsofliquidcondensates AT stefanobo quantitativetheoryforthediffusivedynamicsofliquidcondensates AT anthonyahyman quantitativetheoryforthediffusivedynamicsofliquidcondensates AT christophaweber quantitativetheoryforthediffusivedynamicsofliquidcondensates
_version_	1718440053632401408

Quantitative theory for the diffusive dynamics of liquid condensates

Ejemplares similares