Molecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in Saccharomyces cerevisiae

Abstract The use of lignocellulosic-based fermentation media will be a necessary part of the transition to a circular bio-economy. These media contain many inhibitors to microbial growth, including acetic acid. Under industrially relevant conditions, acetic acid enters the cell predominantly through...

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Autores principales:	Jeroen M. Maertens, Simone Scrima, Matteo Lambrughi, Samuel Genheden, Cecilia Trivellin, Leif A. Eriksson, Elena Papaleo, Lisbeth Olsson, Maurizio Bettiga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb4202100acf44439af78cf73782172f
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spelling	oai:doaj.org-article:eb4202100acf44439af78cf73782172f2021-12-02T16:38:49ZMolecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in Saccharomyces cerevisiae10.1038/s41598-021-96757-y2045-2322https://doaj.org/article/eb4202100acf44439af78cf73782172f2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96757-yhttps://doaj.org/toc/2045-2322Abstract The use of lignocellulosic-based fermentation media will be a necessary part of the transition to a circular bio-economy. These media contain many inhibitors to microbial growth, including acetic acid. Under industrially relevant conditions, acetic acid enters the cell predominantly through passive diffusion across the plasma membrane. The lipid composition of the membrane determines the rate of uptake of acetic acid, and thicker, more rigid membranes impede passive diffusion. We hypothesized that the elongation of glycerophospholipid fatty acids would lead to thicker and more rigid membranes, reducing the influx of acetic acid. Molecular dynamics simulations were used to predict the changes in membrane properties. Heterologous expression of Arabidopsis thaliana genes fatty acid elongase 1 (FAE1) and glycerol-3-phosphate acyltransferase 5 (GPAT5) increased the average fatty acid chain length. However, this did not lead to a reduction in the net uptake rate of acetic acid. Despite successful strain engineering, the net uptake rate of acetic acid did not decrease. We suggest that changes in the relative abundance of certain membrane lipid headgroups could mitigate the effect of longer fatty acid chains, resulting in a higher net uptake rate of acetic acid.Jeroen M. MaertensSimone ScrimaMatteo LambrughiSamuel GenhedenCecilia TrivellinLeif A. ErikssonElena PapaleoLisbeth OlssonMaurizio BettigaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeroen M. Maertens Simone Scrima Matteo Lambrughi Samuel Genheden Cecilia Trivellin Leif A. Eriksson Elena Papaleo Lisbeth Olsson Maurizio Bettiga Molecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in Saccharomyces cerevisiae
description	Abstract The use of lignocellulosic-based fermentation media will be a necessary part of the transition to a circular bio-economy. These media contain many inhibitors to microbial growth, including acetic acid. Under industrially relevant conditions, acetic acid enters the cell predominantly through passive diffusion across the plasma membrane. The lipid composition of the membrane determines the rate of uptake of acetic acid, and thicker, more rigid membranes impede passive diffusion. We hypothesized that the elongation of glycerophospholipid fatty acids would lead to thicker and more rigid membranes, reducing the influx of acetic acid. Molecular dynamics simulations were used to predict the changes in membrane properties. Heterologous expression of Arabidopsis thaliana genes fatty acid elongase 1 (FAE1) and glycerol-3-phosphate acyltransferase 5 (GPAT5) increased the average fatty acid chain length. However, this did not lead to a reduction in the net uptake rate of acetic acid. Despite successful strain engineering, the net uptake rate of acetic acid did not decrease. We suggest that changes in the relative abundance of certain membrane lipid headgroups could mitigate the effect of longer fatty acid chains, resulting in a higher net uptake rate of acetic acid.
format	article
author	Jeroen M. Maertens Simone Scrima Matteo Lambrughi Samuel Genheden Cecilia Trivellin Leif A. Eriksson Elena Papaleo Lisbeth Olsson Maurizio Bettiga
author_facet	Jeroen M. Maertens Simone Scrima Matteo Lambrughi Samuel Genheden Cecilia Trivellin Leif A. Eriksson Elena Papaleo Lisbeth Olsson Maurizio Bettiga
author_sort	Jeroen M. Maertens
title	Molecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in Saccharomyces cerevisiae
title_short	Molecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in Saccharomyces cerevisiae
title_full	Molecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in Saccharomyces cerevisiae
title_fullStr	Molecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in Saccharomyces cerevisiae
title_full_unstemmed	Molecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in Saccharomyces cerevisiae
title_sort	molecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in saccharomyces cerevisiae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eb4202100acf44439af78cf73782172f
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Molecular-dynamics-simulation-guided membrane engineering allows the increase of membrane fatty acid chain length in Saccharomyces cerevisiae

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