Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ

MS2 is a single-stranded RNA bacteriophage that infects its host via adsorption to bacterial pili. Here the authors visualize the MS2 virion with asymmetric cryo-EM reconstruction, revealing that the genome of MS2 adopts a specific structure of asymmetrically distributed stem-loops connected to the...

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Autores principales: Roman I Koning, Josue Gomez-Blanco, Inara Akopjana, Javier Vargas, Andris Kazaks, Kaspars Tars, José María Carazo, Abraham J. Koster
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/eb4699af3d054381a83bc6accac7f614
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Sumario:MS2 is a single-stranded RNA bacteriophage that infects its host via adsorption to bacterial pili. Here the authors visualize the MS2 virion with asymmetric cryo-EM reconstruction, revealing that the genome of MS2 adopts a specific structure of asymmetrically distributed stem-loops connected to the capsid.