New chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo

Abstract It is extremely challenging to perform chemical analyses of the brain, particularly in humans, due to the restricted access to this organ. Imaging techniques are the primary approach used in clinical practice, but they only provide limited information about brain chemistry. Solid-phase micr...

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Autores principales:	Joanna Bogusiewicz, Katarzyna Burlikowska, Kamil Łuczykowski, Karol Jaroch, Marcin Birski, Jacek Furtak, Marek Harat, Janusz Pawliszyn, Barbara Bojko
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb4f2c45f96f46ac9c5ea7b72a5c2420
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spelling	oai:doaj.org-article:eb4f2c45f96f46ac9c5ea7b72a5c24202021-12-02T19:17:05ZNew chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo10.1038/s41598-021-98973-y2045-2322https://doaj.org/article/eb4f2c45f96f46ac9c5ea7b72a5c24202021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98973-yhttps://doaj.org/toc/2045-2322Abstract It is extremely challenging to perform chemical analyses of the brain, particularly in humans, due to the restricted access to this organ. Imaging techniques are the primary approach used in clinical practice, but they only provide limited information about brain chemistry. Solid-phase microextraction (SPME) has been presented recently as a chemical biopsy tool for the study of animal brains. The current work demonstrates for the first time the use of SPME for the spatially resolved sampling of the human brain in vivo. Specially designed multi-probe sampling device was used to simultaneously extract metabolites from the white and grey matter of patients undergoing brain tumor biopsies. Samples were collected by inserting the probes along the planned trajectory of the biopsy needle prior to the procedure, which was followed by metabolomic and lipidomic analyses. The results revealed that studied brain structures were predominantly composed of lipids, while the concentration and diversity of detected metabolites was higher in white than in grey matter. Although the small number of participants in this research precluded conclusions of a biological nature, the results highlight the advantages of the proposed SPME approach, as well as disadvantages that should be addressed in future studies.Joanna BogusiewiczKatarzyna BurlikowskaKamil ŁuczykowskiKarol JarochMarcin BirskiJacek FurtakMarek HaratJanusz PawliszynBarbara BojkoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joanna Bogusiewicz Katarzyna Burlikowska Kamil Łuczykowski Karol Jaroch Marcin Birski Jacek Furtak Marek Harat Janusz Pawliszyn Barbara Bojko New chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo
description	Abstract It is extremely challenging to perform chemical analyses of the brain, particularly in humans, due to the restricted access to this organ. Imaging techniques are the primary approach used in clinical practice, but they only provide limited information about brain chemistry. Solid-phase microextraction (SPME) has been presented recently as a chemical biopsy tool for the study of animal brains. The current work demonstrates for the first time the use of SPME for the spatially resolved sampling of the human brain in vivo. Specially designed multi-probe sampling device was used to simultaneously extract metabolites from the white and grey matter of patients undergoing brain tumor biopsies. Samples were collected by inserting the probes along the planned trajectory of the biopsy needle prior to the procedure, which was followed by metabolomic and lipidomic analyses. The results revealed that studied brain structures were predominantly composed of lipids, while the concentration and diversity of detected metabolites was higher in white than in grey matter. Although the small number of participants in this research precluded conclusions of a biological nature, the results highlight the advantages of the proposed SPME approach, as well as disadvantages that should be addressed in future studies.
format	article
author	Joanna Bogusiewicz Katarzyna Burlikowska Kamil Łuczykowski Karol Jaroch Marcin Birski Jacek Furtak Marek Harat Janusz Pawliszyn Barbara Bojko
author_facet	Joanna Bogusiewicz Katarzyna Burlikowska Kamil Łuczykowski Karol Jaroch Marcin Birski Jacek Furtak Marek Harat Janusz Pawliszyn Barbara Bojko
author_sort	Joanna Bogusiewicz
title	New chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo
title_short	New chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo
title_full	New chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo
title_fullStr	New chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo
title_full_unstemmed	New chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo
title_sort	new chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eb4f2c45f96f46ac9c5ea7b72a5c2420
work_keys_str_mv	AT joannabogusiewicz newchemicalbiopsytoolforspatiallyresolvedprofilingofhumanbraintissueinvivo AT katarzynaburlikowska newchemicalbiopsytoolforspatiallyresolvedprofilingofhumanbraintissueinvivo AT kamilłuczykowski newchemicalbiopsytoolforspatiallyresolvedprofilingofhumanbraintissueinvivo AT karoljaroch newchemicalbiopsytoolforspatiallyresolvedprofilingofhumanbraintissueinvivo AT marcinbirski newchemicalbiopsytoolforspatiallyresolvedprofilingofhumanbraintissueinvivo AT jacekfurtak newchemicalbiopsytoolforspatiallyresolvedprofilingofhumanbraintissueinvivo AT marekharat newchemicalbiopsytoolforspatiallyresolvedprofilingofhumanbraintissueinvivo AT januszpawliszyn newchemicalbiopsytoolforspatiallyresolvedprofilingofhumanbraintissueinvivo AT barbarabojko newchemicalbiopsytoolforspatiallyresolvedprofilingofhumanbraintissueinvivo
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New chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo

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