Improving Cell Penetration of Gold Nanorods by Using an Amphipathic Arginine Rich Peptide

Ana L Riveros,1,2 Cynthia Eggeling,3 Sebastián Riquelme,1 Carolina Adura,1 Carmen López-Iglesias,4 Fanny Guzmán,3 Eyleen Araya,5 Mario Almada,6 Josué Juárez,6 Miguel A Valdez,6 Ignacio A Fuentevilla,1,2,7 Olga López,8 Marcelo...

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Autores principales:	Riveros AL, Eggeling C, Riquelme S, Adura C, López-Iglesias C, Guzmán F, Araya E, Almada M, Juárez J, Valdez MA, Fuentevilla IA, López O, Kogan MJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	gold nanorods cell-penetrating peptides amphipathic arginine rich peptide liposome biological barrier permeation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eb51247877d64e91b2e3b95b0423ebcd
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id	oai:doaj.org-article:eb51247877d64e91b2e3b95b0423ebcd
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	gold nanorods cell-penetrating peptides amphipathic arginine rich peptide liposome biological barrier permeation Medicine (General) R5-920
spellingShingle	gold nanorods cell-penetrating peptides amphipathic arginine rich peptide liposome biological barrier permeation Medicine (General) R5-920 Riveros AL Eggeling C Riquelme S Adura C López-Iglesias C Guzmán F Araya E Almada M Juárez J Valdez MA Fuentevilla IA López O Kogan MJ Improving Cell Penetration of Gold Nanorods by Using an Amphipathic Arginine Rich Peptide
description	Ana L Riveros,1,2 Cynthia Eggeling,3 Sebastián Riquelme,1 Carolina Adura,1 Carmen López-Iglesias,4 Fanny Guzmán,3 Eyleen Araya,5 Mario Almada,6 Josué Juárez,6 Miguel A Valdez,6 Ignacio A Fuentevilla,1,2,7 Olga López,8 Marcelo J Kogan1,2 1Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile; 2Advanced Center for Chronic Diseases (ACCDiS), Universidad de Chile, Santiago, Chile; 3Núcleo de Biotecnología Curauma (NBC), Universidad Católica de Valparaíso, Valparaíso, Chile; 4Microscopy CORE Lab, The Maastricht Multimodal Molecular Imaging Institute FHML, Maastricht University, Maastrich, Netherlands; 5Departamento de Ciencias Quimicas, Facultad de Ciencias Exactas, Universidad Andres Bello, Santiago, Chile; 6Departamento de Física, Universidad de Sonora, Hermosillo, Sonora, México; 7Laboratorio de Investigación en nutrición funcional (LINF), Instituto de Nutrición y Tecnología de los alimentos (INTA), Universidad de Chile, Santiago, Chile; 8Department Surfactants and Nanobiotechnology, Institute for advanced chemistry of Catalonia, Consejo Superior de Investigaciones Científicas (IQAC-CSIC), Barcelona, SpainCorrespondence: Marcelo J Kogan; Ana L RiverosDepartment of Pharmacological and Toxicological Chemistry, University of Chile, Santos Dumont 964, Independencia, Santiago 8380494, ChileTel +56 2 29782897; +56 2 29782918Email mkogan@ciq.uchile.cl; ariveros@uchile.clIntroduction: Gold nanorods are highly reactive, have a large surface-to-volume ratio, and can be functionalized with biomolecules. Gold nanorods can absorb infrared electromagnetic radiation, which is subsequently dispersed as local heat. Gold nanoparticles can be used as powerful tools for the diagnosis and therapy of different diseases. To improve the biological barrier permeation of nanoparticles with low cytotoxicity, in this study, we conjugated gold nanorods with cell-penetrating peptides (oligoarginines) and with the amphipathic peptide CLPFFD.Methods: We studied the interaction of the functionalized gold nanorods with biological membrane models (liposomes) by dynamic light scattering, transmission electron microscopy and the Langmuir balance. Furthermore, we evaluated the effects on cell viability and permeability with an MTS assay and TEM.Results and Discussion: The interaction study by DLS, the Langmuir balance and cryo-TEM support that GNR-Arg7CLPFFD enhances the interactions between GNRs and biological membranes. In addition, cells treated with GNR-Arg7CLPFFD internalized 80% more nanoparticles than cells treated with GNR alone and did not induce cell damage.Conclusion: Our results indicate that incorporation of an amphipathic sequence into oligoarginines for the functionalization of gold nanorods enhances biological membrane nanoparticle interactions and nanoparticle cell permeability with respect to nanorods functionalized with oligoarginine. Overall, functionalized gold nanorods with amphipathic arginine rich peptides might be candidates for improving drug delivery by facilitating biological barrier permeation.Keywords: gold nanorods, cell-penetrating peptides, amphipathic arginine rich peptide, liposome, biological barrier permeation
format	article
author	Riveros AL Eggeling C Riquelme S Adura C López-Iglesias C Guzmán F Araya E Almada M Juárez J Valdez MA Fuentevilla IA López O Kogan MJ
author_facet	Riveros AL Eggeling C Riquelme S Adura C López-Iglesias C Guzmán F Araya E Almada M Juárez J Valdez MA Fuentevilla IA López O Kogan MJ
author_sort	Riveros AL
title	Improving Cell Penetration of Gold Nanorods by Using an Amphipathic Arginine Rich Peptide
title_short	Improving Cell Penetration of Gold Nanorods by Using an Amphipathic Arginine Rich Peptide
title_full	Improving Cell Penetration of Gold Nanorods by Using an Amphipathic Arginine Rich Peptide
title_fullStr	Improving Cell Penetration of Gold Nanorods by Using an Amphipathic Arginine Rich Peptide
title_full_unstemmed	Improving Cell Penetration of Gold Nanorods by Using an Amphipathic Arginine Rich Peptide
title_sort	improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/eb51247877d64e91b2e3b95b0423ebcd
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spelling	oai:doaj.org-article:eb51247877d64e91b2e3b95b0423ebcd2021-12-02T11:32:14ZImproving Cell Penetration of Gold Nanorods by Using an Amphipathic Arginine Rich Peptide1178-2013https://doaj.org/article/eb51247877d64e91b2e3b95b0423ebcd2020-03-01T00:00:00Zhttps://www.dovepress.com/improving-cell-penetration-of-gold-nanorods-by-using-an-amphipathic-ar-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ana L Riveros,1,2 Cynthia Eggeling,3 Sebastián Riquelme,1 Carolina Adura,1 Carmen López-Iglesias,4 Fanny Guzmán,3 Eyleen Araya,5 Mario Almada,6 Josué Juárez,6 Miguel A Valdez,6 Ignacio A Fuentevilla,1,2,7 Olga López,8 Marcelo J Kogan1,2 1Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile; 2Advanced Center for Chronic Diseases (ACCDiS), Universidad de Chile, Santiago, Chile; 3Núcleo de Biotecnología Curauma (NBC), Universidad Católica de Valparaíso, Valparaíso, Chile; 4Microscopy CORE Lab, The Maastricht Multimodal Molecular Imaging Institute FHML, Maastricht University, Maastrich, Netherlands; 5Departamento de Ciencias Quimicas, Facultad de Ciencias Exactas, Universidad Andres Bello, Santiago, Chile; 6Departamento de Física, Universidad de Sonora, Hermosillo, Sonora, México; 7Laboratorio de Investigación en nutrición funcional (LINF), Instituto de Nutrición y Tecnología de los alimentos (INTA), Universidad de Chile, Santiago, Chile; 8Department Surfactants and Nanobiotechnology, Institute for advanced chemistry of Catalonia, Consejo Superior de Investigaciones Científicas (IQAC-CSIC), Barcelona, SpainCorrespondence: Marcelo J Kogan; Ana L RiverosDepartment of Pharmacological and Toxicological Chemistry, University of Chile, Santos Dumont 964, Independencia, Santiago 8380494, ChileTel +56 2 29782897; +56 2 29782918Email mkogan@ciq.uchile.cl; ariveros@uchile.clIntroduction: Gold nanorods are highly reactive, have a large surface-to-volume ratio, and can be functionalized with biomolecules. Gold nanorods can absorb infrared electromagnetic radiation, which is subsequently dispersed as local heat. Gold nanoparticles can be used as powerful tools for the diagnosis and therapy of different diseases. To improve the biological barrier permeation of nanoparticles with low cytotoxicity, in this study, we conjugated gold nanorods with cell-penetrating peptides (oligoarginines) and with the amphipathic peptide CLPFFD.Methods: We studied the interaction of the functionalized gold nanorods with biological membrane models (liposomes) by dynamic light scattering, transmission electron microscopy and the Langmuir balance. Furthermore, we evaluated the effects on cell viability and permeability with an MTS assay and TEM.Results and Discussion: The interaction study by DLS, the Langmuir balance and cryo-TEM support that GNR-Arg7CLPFFD enhances the interactions between GNRs and biological membranes. In addition, cells treated with GNR-Arg7CLPFFD internalized 80% more nanoparticles than cells treated with GNR alone and did not induce cell damage.Conclusion: Our results indicate that incorporation of an amphipathic sequence into oligoarginines for the functionalization of gold nanorods enhances biological membrane nanoparticle interactions and nanoparticle cell permeability with respect to nanorods functionalized with oligoarginine. Overall, functionalized gold nanorods with amphipathic arginine rich peptides might be candidates for improving drug delivery by facilitating biological barrier permeation.Keywords: gold nanorods, cell-penetrating peptides, amphipathic arginine rich peptide, liposome, biological barrier permeationRiveros ALEggeling CRiquelme SAdura CLópez-Iglesias CGuzmán FAraya EAlmada MJuárez JValdez MAFuentevilla IALópez OKogan MJDove Medical Pressarticlegold nanorodscell-penetrating peptidesamphipathic arginine rich peptideliposomebiological barrier permeationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 1837-1851 (2020)

Improving Cell Penetration of Gold Nanorods by Using an Amphipathic Arginine Rich Peptide

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