Antiproliferative and apoptotic activity of glycyrrhizinic acid in MCF-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-TOR/PI3K/Akt signalling pathway

Introduction Glycyrrhizinic acid is a natural product of pharmacological relevance and its anticancer activity against breast cancer cell lines has not been evaluated. Therefore the main purpose of the present study was to investigate the anticancer effects of glycyrrhizinic acid in MCF-7 human brea...

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Autores principales:	Zhen Zhang, Yun Feng, Zhen-Yu Li, Xiao-Zhong Cao
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2018
Materias:	apoptosis breast cancer cell cycle flow cytometry glycyrrhizinic acid Medicine R
Acceso en línea:	https://doaj.org/article/eb521726738c40a284d9e41c9d40b284
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spelling	oai:doaj.org-article:eb521726738c40a284d9e41c9d40b2842021-12-02T16:58:24ZAntiproliferative and apoptotic activity of glycyrrhizinic acid in MCF-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-TOR/PI3K/Akt signalling pathway1734-19221896-915110.5114/aoms.2018.79429https://doaj.org/article/eb521726738c40a284d9e41c9d40b2842018-12-01T00:00:00Zhttps://www.archivesofmedicalscience.com/Antiproliferative-and-apoptotic-activity-of-glycyrrhizinic-acid-in-MCF-7-human-breast,66117,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction Glycyrrhizinic acid is a natural product of pharmacological relevance and its anticancer activity against breast cancer cell lines has not been evaluated. Therefore the main purpose of the present study was to investigate the anticancer effects of glycyrrhizinic acid in MCF-7 human breast cancer cells. Material and methods The MTT assay was used to evaluate the anticancer effects while a clonogenic assay was used to study its effects on colony formation tendency. Flow cytometry was used to study the effects on cell cycle phase distribution and apoptosis. Western blot assay was used to study changes in protein expression of the m-TOR/PI3K/Akt pathway. Results The results indicated that glycyrrhizinic acid caused significant (p < 0.01). The growth inhibitory effects MCF-7 human breast cancer cells. The growth inhibitory effects of glycyrrhizinic acid exhibited concentration-dependent as well as time-dependent growth inhibitory trend. Different doses of glycyrrhizinic acid had a tendency to significantly (p < 0.01) inhibit the colony formation tendency of MCF-7 cells. As compared to the control group, glycyrrhizinic acid-treated cells showed a high percentage of apoptotic cells. Cells treated with a 10, 50 and 100 µM dose of glycyrrhizinic acid led to a 24.3%, 41.5% and 82.1% increase in the sub-G1 phase (apoptotic) cells. Glycyrrhizinic acid also led to significant (p < 0.01) inhibition of cell invasion along with downregulation of m-TOR/PI3K/Akt protein expression. Conclusions Glycyrrhizinic acid inhibited MCF-7 human breast cancer cell growth and therefore may prove essential lead molecule in the treatment of breast cancer.Zhen ZhangYun FengZhen-Yu LiXiao-Zhong CaoTermedia Publishing Housearticleapoptosisbreast cancercell cycleflow cytometryglycyrrhizinic acidapoptosisMedicineRENArchives of Medical Science, Vol 15, Iss 1, Pp 174-182 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis breast cancer cell cycle flow cytometry glycyrrhizinic acid apoptosis Medicine R
spellingShingle	apoptosis breast cancer cell cycle flow cytometry glycyrrhizinic acid apoptosis Medicine R Zhen Zhang Yun Feng Zhen-Yu Li Xiao-Zhong Cao Antiproliferative and apoptotic activity of glycyrrhizinic acid in MCF-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-TOR/PI3K/Akt signalling pathway
description	Introduction Glycyrrhizinic acid is a natural product of pharmacological relevance and its anticancer activity against breast cancer cell lines has not been evaluated. Therefore the main purpose of the present study was to investigate the anticancer effects of glycyrrhizinic acid in MCF-7 human breast cancer cells. Material and methods The MTT assay was used to evaluate the anticancer effects while a clonogenic assay was used to study its effects on colony formation tendency. Flow cytometry was used to study the effects on cell cycle phase distribution and apoptosis. Western blot assay was used to study changes in protein expression of the m-TOR/PI3K/Akt pathway. Results The results indicated that glycyrrhizinic acid caused significant (p < 0.01). The growth inhibitory effects MCF-7 human breast cancer cells. The growth inhibitory effects of glycyrrhizinic acid exhibited concentration-dependent as well as time-dependent growth inhibitory trend. Different doses of glycyrrhizinic acid had a tendency to significantly (p < 0.01) inhibit the colony formation tendency of MCF-7 cells. As compared to the control group, glycyrrhizinic acid-treated cells showed a high percentage of apoptotic cells. Cells treated with a 10, 50 and 100 µM dose of glycyrrhizinic acid led to a 24.3%, 41.5% and 82.1% increase in the sub-G1 phase (apoptotic) cells. Glycyrrhizinic acid also led to significant (p < 0.01) inhibition of cell invasion along with downregulation of m-TOR/PI3K/Akt protein expression. Conclusions Glycyrrhizinic acid inhibited MCF-7 human breast cancer cell growth and therefore may prove essential lead molecule in the treatment of breast cancer.
format	article
author	Zhen Zhang Yun Feng Zhen-Yu Li Xiao-Zhong Cao
author_facet	Zhen Zhang Yun Feng Zhen-Yu Li Xiao-Zhong Cao
author_sort	Zhen Zhang
title	Antiproliferative and apoptotic activity of glycyrrhizinic acid in MCF-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-TOR/PI3K/Akt signalling pathway
title_short	Antiproliferative and apoptotic activity of glycyrrhizinic acid in MCF-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-TOR/PI3K/Akt signalling pathway
title_full	Antiproliferative and apoptotic activity of glycyrrhizinic acid in MCF-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-TOR/PI3K/Akt signalling pathway
title_fullStr	Antiproliferative and apoptotic activity of glycyrrhizinic acid in MCF-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-TOR/PI3K/Akt signalling pathway
title_full_unstemmed	Antiproliferative and apoptotic activity of glycyrrhizinic acid in MCF-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-TOR/PI3K/Akt signalling pathway
title_sort	antiproliferative and apoptotic activity of glycyrrhizinic acid in mcf-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-tor/pi3k/akt signalling pathway
publisher	Termedia Publishing House
publishDate	2018
url	https://doaj.org/article/eb521726738c40a284d9e41c9d40b284
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Antiproliferative and apoptotic activity of glycyrrhizinic acid in MCF-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-TOR/PI3K/Akt signalling pathway

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