First report on establishment and characterization of a carcinosarcoma tumour cell line model of the bladder

Abstract Carcinosarcoma of the urinary bladder is a very rare and aggressive subtype of bladder cancer with poor prognosis. Characteristically carcinosarcomas exhibit biphasic nature with both epithelial and mesenchymal differentiation. Limited information is available regarding its clinical feature...

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Autores principales:	Johannes Eberhard, Daniela Hirsch, Oliver Schilling, Wilhelm G. Dirks, Feng Guo, Alice Fabarius, Felix Rückert, Christoph Reißfelder, Peter Hohenberger, Prama Pallavi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb599c0904f24b9c9e029802e5c2223b
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spelling	oai:doaj.org-article:eb599c0904f24b9c9e029802e5c2223b2021-12-02T17:05:12ZFirst report on establishment and characterization of a carcinosarcoma tumour cell line model of the bladder10.1038/s41598-021-85400-52045-2322https://doaj.org/article/eb599c0904f24b9c9e029802e5c2223b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85400-5https://doaj.org/toc/2045-2322Abstract Carcinosarcoma of the urinary bladder is a very rare and aggressive subtype of bladder cancer with poor prognosis. Characteristically carcinosarcomas exhibit biphasic nature with both epithelial and mesenchymal differentiation. Limited information is available regarding its clinical features and appropriate treatments due to its rarity. Development of tumour models can further our understanding of bladder carcinosarcoma. We report establishment and characterization of the first-ever bladder carcinosarcoma cell line MaS-3. It is established by the outgrow method from 86 year-old caucasian male who underwent a radical pelvic resection after neoadjuvant radiotherapy. MaS-3 showed carcinosarcoma profile with high conformity with to the original tumour in terms of immunocytochemistry. Proteome analysis also aligned the MaS-3 cell line with the carcinosarcoma specimen rather than corresponding non-malignant tissue. Chemotherapy sensitivity testing revealed a great sensitivity of MaS-3 growth to 5-Fluorouracil, Gemcitabine and Cisplatin, with almost no impact of Irinotecan. Additionally, the suitability of MaS-3 for 3D in vitro experiments was also demonstrated. The newly established cell line MaS-3 shows typical characteristics of the tumour and may thus be a useful in vitro model system for studying the tumour biology and developing future of treatments of this rare but very aggressive entity.Johannes EberhardDaniela HirschOliver SchillingWilhelm G. DirksFeng GuoAlice FabariusFelix RückertChristoph ReißfelderPeter HohenbergerPrama PallaviNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johannes Eberhard Daniela Hirsch Oliver Schilling Wilhelm G. Dirks Feng Guo Alice Fabarius Felix Rückert Christoph Reißfelder Peter Hohenberger Prama Pallavi First report on establishment and characterization of a carcinosarcoma tumour cell line model of the bladder
description	Abstract Carcinosarcoma of the urinary bladder is a very rare and aggressive subtype of bladder cancer with poor prognosis. Characteristically carcinosarcomas exhibit biphasic nature with both epithelial and mesenchymal differentiation. Limited information is available regarding its clinical features and appropriate treatments due to its rarity. Development of tumour models can further our understanding of bladder carcinosarcoma. We report establishment and characterization of the first-ever bladder carcinosarcoma cell line MaS-3. It is established by the outgrow method from 86 year-old caucasian male who underwent a radical pelvic resection after neoadjuvant radiotherapy. MaS-3 showed carcinosarcoma profile with high conformity with to the original tumour in terms of immunocytochemistry. Proteome analysis also aligned the MaS-3 cell line with the carcinosarcoma specimen rather than corresponding non-malignant tissue. Chemotherapy sensitivity testing revealed a great sensitivity of MaS-3 growth to 5-Fluorouracil, Gemcitabine and Cisplatin, with almost no impact of Irinotecan. Additionally, the suitability of MaS-3 for 3D in vitro experiments was also demonstrated. The newly established cell line MaS-3 shows typical characteristics of the tumour and may thus be a useful in vitro model system for studying the tumour biology and developing future of treatments of this rare but very aggressive entity.
format	article
author	Johannes Eberhard Daniela Hirsch Oliver Schilling Wilhelm G. Dirks Feng Guo Alice Fabarius Felix Rückert Christoph Reißfelder Peter Hohenberger Prama Pallavi
author_facet	Johannes Eberhard Daniela Hirsch Oliver Schilling Wilhelm G. Dirks Feng Guo Alice Fabarius Felix Rückert Christoph Reißfelder Peter Hohenberger Prama Pallavi
author_sort	Johannes Eberhard
title	First report on establishment and characterization of a carcinosarcoma tumour cell line model of the bladder
title_short	First report on establishment and characterization of a carcinosarcoma tumour cell line model of the bladder
title_full	First report on establishment and characterization of a carcinosarcoma tumour cell line model of the bladder
title_fullStr	First report on establishment and characterization of a carcinosarcoma tumour cell line model of the bladder
title_full_unstemmed	First report on establishment and characterization of a carcinosarcoma tumour cell line model of the bladder
title_sort	first report on establishment and characterization of a carcinosarcoma tumour cell line model of the bladder
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eb599c0904f24b9c9e029802e5c2223b
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First report on establishment and characterization of a carcinosarcoma tumour cell line model of the bladder

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