Concerted evolution in the ribosomal RNA cistron.

<h4>Background</h4>Gene conversion is the mechanism proposed to be responsible for the homogenization of multigene families such as the nuclear ribosomal gene clusters. This concerted evolutionary process prevents individual genes in gene clusters from accumulating mutations. The mechani...

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Autores principales:	Kershney Naidoo, Emma T Steenkamp, Martin P A Coetzee, Michael J Wingfield, Brenda D Wingfield
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb60008f5b8844db81385801c33f3b12
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spelling	oai:doaj.org-article:eb60008f5b8844db81385801c33f3b122021-11-18T07:53:45ZConcerted evolution in the ribosomal RNA cistron.1932-620310.1371/journal.pone.0059355https://doaj.org/article/eb60008f5b8844db81385801c33f3b122013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23555022/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Gene conversion is the mechanism proposed to be responsible for the homogenization of multigene families such as the nuclear ribosomal gene clusters. This concerted evolutionary process prevents individual genes in gene clusters from accumulating mutations. The mechanism responsible for concerted evolution is not well understood but recombination during meiosis has been hypothesized to play a significant role in this homogenization. In this study we tested the hypothesis of unequal crossing over playing a significant role in gene conversion events within the ribosomal RNA cistron during meiosis, mitosis or both life stages in the fungal tree pathogen Ceratocystis manginecans.<h4>Methods</h4>Ceratocystis manginecans, a haploid ascomycete, reproduces homothallically and was found to have two distinct sequences within the internally transcribed spacer (ITS) region of the ribosomal RNA cistron. The different ITS types were scored using PCR-RFLP assays and chi-square analyses to determine the level of significance of the changes in the ratios of the ITS types.<h4>Results</h4>The relative ratios of the two ITS sequence types changed when the fungal isolates were cultured vegetatively or allowed to produced sexual structures and spores. These active changes were shown to occur more frequently during meiosis than mitosis.<h4>Conclusion</h4>The evidence presented provides concrete support for homogenization in the rRNA gene clusters found in this fungus and that the most reasonable explanation for this process is unequal crossing over.Kershney NaidooEmma T SteenkampMartin P A CoetzeeMichael J WingfieldBrenda D WingfieldPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e59355 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kershney Naidoo Emma T Steenkamp Martin P A Coetzee Michael J Wingfield Brenda D Wingfield Concerted evolution in the ribosomal RNA cistron.
description	<h4>Background</h4>Gene conversion is the mechanism proposed to be responsible for the homogenization of multigene families such as the nuclear ribosomal gene clusters. This concerted evolutionary process prevents individual genes in gene clusters from accumulating mutations. The mechanism responsible for concerted evolution is not well understood but recombination during meiosis has been hypothesized to play a significant role in this homogenization. In this study we tested the hypothesis of unequal crossing over playing a significant role in gene conversion events within the ribosomal RNA cistron during meiosis, mitosis or both life stages in the fungal tree pathogen Ceratocystis manginecans.<h4>Methods</h4>Ceratocystis manginecans, a haploid ascomycete, reproduces homothallically and was found to have two distinct sequences within the internally transcribed spacer (ITS) region of the ribosomal RNA cistron. The different ITS types were scored using PCR-RFLP assays and chi-square analyses to determine the level of significance of the changes in the ratios of the ITS types.<h4>Results</h4>The relative ratios of the two ITS sequence types changed when the fungal isolates were cultured vegetatively or allowed to produced sexual structures and spores. These active changes were shown to occur more frequently during meiosis than mitosis.<h4>Conclusion</h4>The evidence presented provides concrete support for homogenization in the rRNA gene clusters found in this fungus and that the most reasonable explanation for this process is unequal crossing over.
format	article
author	Kershney Naidoo Emma T Steenkamp Martin P A Coetzee Michael J Wingfield Brenda D Wingfield
author_facet	Kershney Naidoo Emma T Steenkamp Martin P A Coetzee Michael J Wingfield Brenda D Wingfield
author_sort	Kershney Naidoo
title	Concerted evolution in the ribosomal RNA cistron.
title_short	Concerted evolution in the ribosomal RNA cistron.
title_full	Concerted evolution in the ribosomal RNA cistron.
title_fullStr	Concerted evolution in the ribosomal RNA cistron.
title_full_unstemmed	Concerted evolution in the ribosomal RNA cistron.
title_sort	concerted evolution in the ribosomal rna cistron.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/eb60008f5b8844db81385801c33f3b12
work_keys_str_mv	AT kershneynaidoo concertedevolutionintheribosomalrnacistron AT emmatsteenkamp concertedevolutionintheribosomalrnacistron AT martinpacoetzee concertedevolutionintheribosomalrnacistron AT michaeljwingfield concertedevolutionintheribosomalrnacistron AT brendadwingfield concertedevolutionintheribosomalrnacistron
_version_	1718422800828465152

Concerted evolution in the ribosomal RNA cistron.

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