Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer

Abstract Ribosome inactivating proteins (RIPs) are RNA N-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28S rRNA. These enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. Recently, we rep...

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Autores principales:	Walter J. Lapadula, Paula L. Marcet, María L. Mascotti, M. Virginia Sanchez-Puerta, Maximiliano Juri Ayub
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb64b55e5be14a56912b7b6edd203acd
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spelling	oai:doaj.org-article:eb64b55e5be14a56912b7b6edd203acd2021-12-02T12:30:54ZMetazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer10.1038/s41598-017-01859-12045-2322https://doaj.org/article/eb64b55e5be14a56912b7b6edd203acd2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01859-1https://doaj.org/toc/2045-2322Abstract Ribosome inactivating proteins (RIPs) are RNA N-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28S rRNA. These enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. Recently, we reported for the first time in silico evidence of RIP encoding genes in metazoans, in two closely related species of insects: Aedes aegypti and Culex quinquefasciatus. Here, we have experimentally confirmed the presence of these genes in mosquitoes and attempted to unveil their evolutionary history. A detailed study was conducted, including evaluation of taxonomic distribution, phylogenetic inferences and microsynteny analyses, indicating that mosquito RIP genes derived from a single Horizontal Gene Transfer (HGT) event, probably from a cyanobacterial donor species. Moreover, evolutionary analyses show that, after the HGT event, these genes evolved under purifying selection, strongly suggesting they play functional roles in these organisms.Walter J. LapadulaPaula L. MarcetMaría L. MascottiM. Virginia Sanchez-PuertaMaximiliano Juri AyubNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Walter J. Lapadula Paula L. Marcet María L. Mascotti M. Virginia Sanchez-Puerta Maximiliano Juri Ayub Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer
description	Abstract Ribosome inactivating proteins (RIPs) are RNA N-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28S rRNA. These enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. Recently, we reported for the first time in silico evidence of RIP encoding genes in metazoans, in two closely related species of insects: Aedes aegypti and Culex quinquefasciatus. Here, we have experimentally confirmed the presence of these genes in mosquitoes and attempted to unveil their evolutionary history. A detailed study was conducted, including evaluation of taxonomic distribution, phylogenetic inferences and microsynteny analyses, indicating that mosquito RIP genes derived from a single Horizontal Gene Transfer (HGT) event, probably from a cyanobacterial donor species. Moreover, evolutionary analyses show that, after the HGT event, these genes evolved under purifying selection, strongly suggesting they play functional roles in these organisms.
format	article
author	Walter J. Lapadula Paula L. Marcet María L. Mascotti M. Virginia Sanchez-Puerta Maximiliano Juri Ayub
author_facet	Walter J. Lapadula Paula L. Marcet María L. Mascotti M. Virginia Sanchez-Puerta Maximiliano Juri Ayub
author_sort	Walter J. Lapadula
title	Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer
title_short	Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer
title_full	Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer
title_fullStr	Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer
title_full_unstemmed	Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer
title_sort	metazoan ribosome inactivating protein encoding genes acquired by horizontal gene transfer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/eb64b55e5be14a56912b7b6edd203acd
work_keys_str_mv	AT walterjlapadula metazoanribosomeinactivatingproteinencodinggenesacquiredbyhorizontalgenetransfer AT paulalmarcet metazoanribosomeinactivatingproteinencodinggenesacquiredbyhorizontalgenetransfer AT marialmascotti metazoanribosomeinactivatingproteinencodinggenesacquiredbyhorizontalgenetransfer AT mvirginiasanchezpuerta metazoanribosomeinactivatingproteinencodinggenesacquiredbyhorizontalgenetransfer AT maximilianojuriayub metazoanribosomeinactivatingproteinencodinggenesacquiredbyhorizontalgenetransfer
_version_	1718394311748354048

Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer

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