Antiviral mechanism of polyanionic carbosilane dendrimers against HIV-1

Enrique Vacas-Córdoba,1–4 Marek Maly,5,6 Francisco J De la Mata,4,7 Rafael Gómez,4,7 Marjorie Pion,1–4 Mª Ángeles Muñoz-Fernández1–41Molecular Immunobiology Laboratory, General Universitary Hospital Gregori...

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Autores principales:	Vacas-Córdoba E, Maly M, De la Mata FJ, Gómez R, Pion M, Muñoz-Fernández MA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	carbosilane dendrimer HIV mechanism microbicide nanotechnology Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eb67904b6f5140509b9ffcbbf142d2c9
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spelling	oai:doaj.org-article:eb67904b6f5140509b9ffcbbf142d2c92021-12-02T02:28:08ZAntiviral mechanism of polyanionic carbosilane dendrimers against HIV-11178-2013https://doaj.org/article/eb67904b6f5140509b9ffcbbf142d2c92016-04-01T00:00:00Zhttps://www.dovepress.com/antiviral-mechanism-of-polyanionic-carbosilane-dendrimers-against-hiv--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Enrique Vacas-Córdoba,1–4 Marek Maly,5,6 Francisco J De la Mata,4,7 Rafael Gómez,4,7 Marjorie Pion,1–4 Mª Ángeles Muñoz-Fernández1–41Molecular Immunobiology Laboratory, General Universitary Hospital Gregorio Marañon, 2Health Research Institute Gregorio Marañon, 3Spanish HIV HGM BioBanK, 4Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; 5Faculty of Science, Jan Evangelista Purkynĕ University, Ústí nad Labem, Czech Republic; 6Laboratory of Applied Mathematics and Physics (LaMFI), University of Applied Sciences and Arts of Southern Switzerland, Manno, Switzerland; 7Dendrimers for Biomedical Applications Group (BioInDen), University of Alcalá, Alcalá de Henares, Madrid, Spain Abstract: Nanotechnology-derived platforms, such as dendrimers, are very attractive in several biological applications. In the case of human immunodeficiency virus (HIV) infection, polyanionic carbosilane dendrimers have shown great potential as antiviral agents in the development of novel microbicides to prevent the sexual transmission of HIV-1. In this work, we studied the mechanism of two sulfated and naphthylsulfonated functionalized carbosilane dendrimers, G3-S16 and G2-NF16. They are able to inhibit viral infection at fusion and thus at the entry step. Both compounds impede the binding of viral particles to target cell surface and membrane fusion through the blockage of gp120–CD4 interaction. In addition, and for the first time, we demonstrate that dendrimers can inhibit cell-to-cell HIV transmission and difficult infectious synapse formation. Thus, carbosilane dendrimers’ mode of action is a multifactorial process targeting several proteins from viral envelope and from host cells that could block HIV infection at different stages during the first step of infection.Keywords: carbosilane dendrimer, HIV, mechanism, microbicide, nanotechnologyVacas-Córdoba EMaly MDe la Mata FJGómez RPion MMuñoz-Fernández MADove Medical Pressarticlecarbosilane dendrimerHIVmechanismmicrobicidenanotechnologyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1281-1294 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbosilane dendrimer HIV mechanism microbicide nanotechnology Medicine (General) R5-920
spellingShingle	carbosilane dendrimer HIV mechanism microbicide nanotechnology Medicine (General) R5-920 Vacas-Córdoba E Maly M De la Mata FJ Gómez R Pion M Muñoz-Fernández MA Antiviral mechanism of polyanionic carbosilane dendrimers against HIV-1
description	Enrique Vacas-Córdoba,1–4 Marek Maly,5,6 Francisco J De la Mata,4,7 Rafael Gómez,4,7 Marjorie Pion,1–4 Mª Ángeles Muñoz-Fernández1–41Molecular Immunobiology Laboratory, General Universitary Hospital Gregorio Marañon, 2Health Research Institute Gregorio Marañon, 3Spanish HIV HGM BioBanK, 4Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; 5Faculty of Science, Jan Evangelista Purkynĕ University, Ústí nad Labem, Czech Republic; 6Laboratory of Applied Mathematics and Physics (LaMFI), University of Applied Sciences and Arts of Southern Switzerland, Manno, Switzerland; 7Dendrimers for Biomedical Applications Group (BioInDen), University of Alcalá, Alcalá de Henares, Madrid, Spain Abstract: Nanotechnology-derived platforms, such as dendrimers, are very attractive in several biological applications. In the case of human immunodeficiency virus (HIV) infection, polyanionic carbosilane dendrimers have shown great potential as antiviral agents in the development of novel microbicides to prevent the sexual transmission of HIV-1. In this work, we studied the mechanism of two sulfated and naphthylsulfonated functionalized carbosilane dendrimers, G3-S16 and G2-NF16. They are able to inhibit viral infection at fusion and thus at the entry step. Both compounds impede the binding of viral particles to target cell surface and membrane fusion through the blockage of gp120–CD4 interaction. In addition, and for the first time, we demonstrate that dendrimers can inhibit cell-to-cell HIV transmission and difficult infectious synapse formation. Thus, carbosilane dendrimers’ mode of action is a multifactorial process targeting several proteins from viral envelope and from host cells that could block HIV infection at different stages during the first step of infection.Keywords: carbosilane dendrimer, HIV, mechanism, microbicide, nanotechnology
format	article
author	Vacas-Córdoba E Maly M De la Mata FJ Gómez R Pion M Muñoz-Fernández MA
author_facet	Vacas-Córdoba E Maly M De la Mata FJ Gómez R Pion M Muñoz-Fernández MA
author_sort	Vacas-Córdoba E
title	Antiviral mechanism of polyanionic carbosilane dendrimers against HIV-1
title_short	Antiviral mechanism of polyanionic carbosilane dendrimers against HIV-1
title_full	Antiviral mechanism of polyanionic carbosilane dendrimers against HIV-1
title_fullStr	Antiviral mechanism of polyanionic carbosilane dendrimers against HIV-1
title_full_unstemmed	Antiviral mechanism of polyanionic carbosilane dendrimers against HIV-1
title_sort	antiviral mechanism of polyanionic carbosilane dendrimers against hiv-1
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/eb67904b6f5140509b9ffcbbf142d2c9
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Antiviral mechanism of polyanionic carbosilane dendrimers against HIV-1

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