Medium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and Δ9-tetrahydrocannabinol

Abstract In light of legislative changes and the widespread use of cannabis as a recreational and medicinal drug, delayed effects of cannabis upon brief exposure during embryonic development are of high interest as early pregnancies often go undetected. Here, zebrafish embryos were exposed to cannab...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Richard Kanyo, Md Ruhul Amin, Laszlo F. Locskai, Danika D. Bouvier, Alexandria M. Olthuis, W. Ted Allison, Declan W. Ali
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb7b922cbe2f4b83b92b7e09ca9ec140
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eb7b922cbe2f4b83b92b7e09ca9ec140
record_format	dspace
spelling	oai:doaj.org-article:eb7b922cbe2f4b83b92b7e09ca9ec1402021-12-02T18:24:53ZMedium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and Δ9-tetrahydrocannabinol10.1038/s41598-021-90902-32045-2322https://doaj.org/article/eb7b922cbe2f4b83b92b7e09ca9ec1402021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90902-3https://doaj.org/toc/2045-2322Abstract In light of legislative changes and the widespread use of cannabis as a recreational and medicinal drug, delayed effects of cannabis upon brief exposure during embryonic development are of high interest as early pregnancies often go undetected. Here, zebrafish embryos were exposed to cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC) until the end of gastrulation (1–10 h post-fertilization) and analyzed later in development (4–5 days post-fertilization). In order to measure neural activity, we implemented Calcium-Modulated Photoactivatable Ratiometric Integrator (CaMPARI) and optimized the protocol for a 96-well format complemented by locomotor analysis. Our results revealed that neural activity was decreased by CBD more than THC. At higher doses, both cannabinoids could dramatically reduce neural activity and locomotor activity. Interestingly, the decrease was more pronounced when CBD and THC were combined. At the receptor level, CBD-mediated reduction of locomotor activity was partially prevented using cannabinoid type 1 and 2 receptor inhibitors. Overall, we report that CBD toxicity occurs via two cannabinoid receptors and is synergistically enhanced by THC exposure to negatively impact neural activity late in larval development. Future studies are warranted to reveal other cannabinoids and their receptors to understand the implications of cannabis consumption on fetal development.Richard KanyoMd Ruhul AminLaszlo F. LocskaiDanika D. BouvierAlexandria M. OlthuisW. Ted AllisonDeclan W. AliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Richard Kanyo Md Ruhul Amin Laszlo F. Locskai Danika D. Bouvier Alexandria M. Olthuis W. Ted Allison Declan W. Ali Medium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and Δ9-tetrahydrocannabinol
description	Abstract In light of legislative changes and the widespread use of cannabis as a recreational and medicinal drug, delayed effects of cannabis upon brief exposure during embryonic development are of high interest as early pregnancies often go undetected. Here, zebrafish embryos were exposed to cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC) until the end of gastrulation (1–10 h post-fertilization) and analyzed later in development (4–5 days post-fertilization). In order to measure neural activity, we implemented Calcium-Modulated Photoactivatable Ratiometric Integrator (CaMPARI) and optimized the protocol for a 96-well format complemented by locomotor analysis. Our results revealed that neural activity was decreased by CBD more than THC. At higher doses, both cannabinoids could dramatically reduce neural activity and locomotor activity. Interestingly, the decrease was more pronounced when CBD and THC were combined. At the receptor level, CBD-mediated reduction of locomotor activity was partially prevented using cannabinoid type 1 and 2 receptor inhibitors. Overall, we report that CBD toxicity occurs via two cannabinoid receptors and is synergistically enhanced by THC exposure to negatively impact neural activity late in larval development. Future studies are warranted to reveal other cannabinoids and their receptors to understand the implications of cannabis consumption on fetal development.
format	article
author	Richard Kanyo Md Ruhul Amin Laszlo F. Locskai Danika D. Bouvier Alexandria M. Olthuis W. Ted Allison Declan W. Ali
author_facet	Richard Kanyo Md Ruhul Amin Laszlo F. Locskai Danika D. Bouvier Alexandria M. Olthuis W. Ted Allison Declan W. Ali
author_sort	Richard Kanyo
title	Medium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and Δ9-tetrahydrocannabinol
title_short	Medium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and Δ9-tetrahydrocannabinol
title_full	Medium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and Δ9-tetrahydrocannabinol
title_fullStr	Medium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and Δ9-tetrahydrocannabinol
title_full_unstemmed	Medium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and Δ9-tetrahydrocannabinol
title_sort	medium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and δ9-tetrahydrocannabinol
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eb7b922cbe2f4b83b92b7e09ca9ec140
work_keys_str_mv	AT richardkanyo mediumthroughputzebrafishoptogeneticplatformidentifiesdeficitsinsubsequentneuralactivityfollowingbriefearlyexposuretocannabidiolandd9tetrahydrocannabinol AT mdruhulamin mediumthroughputzebrafishoptogeneticplatformidentifiesdeficitsinsubsequentneuralactivityfollowingbriefearlyexposuretocannabidiolandd9tetrahydrocannabinol AT laszloflocskai mediumthroughputzebrafishoptogeneticplatformidentifiesdeficitsinsubsequentneuralactivityfollowingbriefearlyexposuretocannabidiolandd9tetrahydrocannabinol AT danikadbouvier mediumthroughputzebrafishoptogeneticplatformidentifiesdeficitsinsubsequentneuralactivityfollowingbriefearlyexposuretocannabidiolandd9tetrahydrocannabinol AT alexandriamolthuis mediumthroughputzebrafishoptogeneticplatformidentifiesdeficitsinsubsequentneuralactivityfollowingbriefearlyexposuretocannabidiolandd9tetrahydrocannabinol AT wtedallison mediumthroughputzebrafishoptogeneticplatformidentifiesdeficitsinsubsequentneuralactivityfollowingbriefearlyexposuretocannabidiolandd9tetrahydrocannabinol AT declanwali mediumthroughputzebrafishoptogeneticplatformidentifiesdeficitsinsubsequentneuralactivityfollowingbriefearlyexposuretocannabidiolandd9tetrahydrocannabinol
_version_	1718378132913782784

Medium-throughput zebrafish optogenetic platform identifies deficits in subsequent neural activity following brief early exposure to cannabidiol and Δ9-tetrahydrocannabinol

Ejemplares similares