An abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis.

Plant stomata function in innate immunity against bacterial invasion and abscisic acid (ABA) has been suggested to regulate this process. Using genetic, biochemical, and pharmacological approaches, we demonstrate that (i) the Arabidopsis thaliana nine-specific-lipoxygenase encoding gene, LOX1, which...

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Autores principales:	Jean-Luc Montillet, Nathalie Leonhardt, Samuel Mondy, Sylvain Tranchimand, Dominique Rumeau, Marie Boudsocq, Ana Victoria Garcia, Thierry Douki, Jean Bigeard, Christiane Laurière, Anne Chevalier, Carmen Castresana, Heribert Hirt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/eb85d1aecdd843b799f15ba64abaf177
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spelling	oai:doaj.org-article:eb85d1aecdd843b799f15ba64abaf1772021-11-18T05:37:12ZAn abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis.1544-91731545-788510.1371/journal.pbio.1001513https://doaj.org/article/eb85d1aecdd843b799f15ba64abaf1772013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23526882/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Plant stomata function in innate immunity against bacterial invasion and abscisic acid (ABA) has been suggested to regulate this process. Using genetic, biochemical, and pharmacological approaches, we demonstrate that (i) the Arabidopsis thaliana nine-specific-lipoxygenase encoding gene, LOX1, which is expressed in guard cells, is required to trigger stomatal closure in response to both bacteria and the pathogen-associated molecular pattern flagellin peptide flg22; (ii) LOX1 participates in stomatal defense; (iii) polyunsaturated fatty acids, the LOX substrates, trigger stomatal closure; (iv) the LOX products, fatty acid hydroperoxides, or reactive electrophile oxylipins induce stomatal closure; and (v) the flg22-mediated stomatal closure is conveyed by both LOX1 and the mitogen-activated protein kinases MPK3 and MPK6 and involves salicylic acid whereas the ABA-induced process depends on the protein kinases OST1, MPK9, or MPK12. Finally, we show that the oxylipin and the ABA pathways converge at the level of the anion channel SLAC1 to regulate stomatal closure. Collectively, our results demonstrate that early biotic signaling in guard cells is an ABA-independent process revealing a novel function of LOX1-dependent stomatal pathway in plant immunity.Jean-Luc MontilletNathalie LeonhardtSamuel MondySylvain TranchimandDominique RumeauMarie BoudsocqAna Victoria GarciaThierry DoukiJean BigeardChristiane LaurièreAnne ChevalierCarmen CastresanaHeribert HirtPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 11, Iss 3, p e1001513 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Jean-Luc Montillet Nathalie Leonhardt Samuel Mondy Sylvain Tranchimand Dominique Rumeau Marie Boudsocq Ana Victoria Garcia Thierry Douki Jean Bigeard Christiane Laurière Anne Chevalier Carmen Castresana Heribert Hirt An abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis.
description	Plant stomata function in innate immunity against bacterial invasion and abscisic acid (ABA) has been suggested to regulate this process. Using genetic, biochemical, and pharmacological approaches, we demonstrate that (i) the Arabidopsis thaliana nine-specific-lipoxygenase encoding gene, LOX1, which is expressed in guard cells, is required to trigger stomatal closure in response to both bacteria and the pathogen-associated molecular pattern flagellin peptide flg22; (ii) LOX1 participates in stomatal defense; (iii) polyunsaturated fatty acids, the LOX substrates, trigger stomatal closure; (iv) the LOX products, fatty acid hydroperoxides, or reactive electrophile oxylipins induce stomatal closure; and (v) the flg22-mediated stomatal closure is conveyed by both LOX1 and the mitogen-activated protein kinases MPK3 and MPK6 and involves salicylic acid whereas the ABA-induced process depends on the protein kinases OST1, MPK9, or MPK12. Finally, we show that the oxylipin and the ABA pathways converge at the level of the anion channel SLAC1 to regulate stomatal closure. Collectively, our results demonstrate that early biotic signaling in guard cells is an ABA-independent process revealing a novel function of LOX1-dependent stomatal pathway in plant immunity.
format	article
author	Jean-Luc Montillet Nathalie Leonhardt Samuel Mondy Sylvain Tranchimand Dominique Rumeau Marie Boudsocq Ana Victoria Garcia Thierry Douki Jean Bigeard Christiane Laurière Anne Chevalier Carmen Castresana Heribert Hirt
author_facet	Jean-Luc Montillet Nathalie Leonhardt Samuel Mondy Sylvain Tranchimand Dominique Rumeau Marie Boudsocq Ana Victoria Garcia Thierry Douki Jean Bigeard Christiane Laurière Anne Chevalier Carmen Castresana Heribert Hirt
author_sort	Jean-Luc Montillet
title	An abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis.
title_short	An abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis.
title_full	An abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis.
title_fullStr	An abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis.
title_full_unstemmed	An abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis.
title_sort	abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in arabidopsis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/eb85d1aecdd843b799f15ba64abaf177
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An abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis.

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