Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase

Here, the authors use solid-state NMR and EPR measurements to characterise the ATP hydrolysis transition state of the oligomeric bacterial DnaB helicase from Helicobacter pylori, which was trapped by using aluminium fluoride as a chemical mimic. They identify protein protons that coordinate to the p...

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Autores principales:	Alexander A. Malär, Nino Wili, Laura A. Völker, Maria I. Kozlova, Riccardo Cadalbert, Alexander Däpp, Marco E. Weber, Johannes Zehnder, Gunnar Jeschke, Hellmut Eckert, Anja Böckmann, Daniel Klose, Armen Y. Mulkidjanian, Beat H. Meier, Thomas Wiegand
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/eb891282fac642d58cd86a545184715e
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spelling	oai:doaj.org-article:eb891282fac642d58cd86a545184715e2021-12-02T19:12:25ZSpectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase10.1038/s41467-021-25599-z2041-1723https://doaj.org/article/eb891282fac642d58cd86a545184715e2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25599-zhttps://doaj.org/toc/2041-1723Here, the authors use solid-state NMR and EPR measurements to characterise the ATP hydrolysis transition state of the oligomeric bacterial DnaB helicase from Helicobacter pylori, which was trapped by using aluminium fluoride as a chemical mimic. They identify protein protons that coordinate to the phosphate groups of ADP and DNA and observe that the aluminium fluoride unit is highly mobile and fast-rotating.Alexander A. MalärNino WiliLaura A. VölkerMaria I. KozlovaRiccardo CadalbertAlexander DäppMarco E. WeberJohannes ZehnderGunnar JeschkeHellmut EckertAnja BöckmannDaniel KloseArmen Y. MulkidjanianBeat H. MeierThomas WiegandNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alexander A. Malär Nino Wili Laura A. Völker Maria I. Kozlova Riccardo Cadalbert Alexander Däpp Marco E. Weber Johannes Zehnder Gunnar Jeschke Hellmut Eckert Anja Böckmann Daniel Klose Armen Y. Mulkidjanian Beat H. Meier Thomas Wiegand Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase
description	Here, the authors use solid-state NMR and EPR measurements to characterise the ATP hydrolysis transition state of the oligomeric bacterial DnaB helicase from Helicobacter pylori, which was trapped by using aluminium fluoride as a chemical mimic. They identify protein protons that coordinate to the phosphate groups of ADP and DNA and observe that the aluminium fluoride unit is highly mobile and fast-rotating.
format	article
author	Alexander A. Malär Nino Wili Laura A. Völker Maria I. Kozlova Riccardo Cadalbert Alexander Däpp Marco E. Weber Johannes Zehnder Gunnar Jeschke Hellmut Eckert Anja Böckmann Daniel Klose Armen Y. Mulkidjanian Beat H. Meier Thomas Wiegand
author_facet	Alexander A. Malär Nino Wili Laura A. Völker Maria I. Kozlova Riccardo Cadalbert Alexander Däpp Marco E. Weber Johannes Zehnder Gunnar Jeschke Hellmut Eckert Anja Böckmann Daniel Klose Armen Y. Mulkidjanian Beat H. Meier Thomas Wiegand
author_sort	Alexander A. Malär
title	Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase
title_short	Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase
title_full	Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase
title_fullStr	Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase
title_full_unstemmed	Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase
title_sort	spectroscopic glimpses of the transition state of atp hydrolysis trapped in a bacterial dnab helicase
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eb891282fac642d58cd86a545184715e
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Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase

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