Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.

Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique under different osmotic pressures (Π), and est...

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Autores principales:	Malay Kumar Sarkar, Mohammad Abu Sayem Karal, Marzuk Ahmed, Md Kabir Ahamed, Shareef Ahammed, Sabrina Sharmin, Sayed Ul Alam Shibly
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb8f88e3f7d04d3ea6507fb9b41af263
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spelling	oai:doaj.org-article:eb8f88e3f7d04d3ea6507fb9b41af2632021-11-25T06:19:13ZEffects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.1932-620310.1371/journal.pone.0251690https://doaj.org/article/eb8f88e3f7d04d3ea6507fb9b41af2632021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251690https://doaj.org/toc/1932-6203Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique under different osmotic pressures (Π), and estimated the membrane tension due to Π. Two categories of GUVs were used in this study. One was prepared with a mixture of dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylcholine (DOPC) and cholesterol (chol) for obtaining more biological relevance while other with a mixture of DOPG and DOPC, with specific molar ratios. We determined the rate constant (kp) of rupture of DOPG/DOPC/chol (46/39/15)-GUVs and DOPG/DOPC (40/60)-GUVs induced by constant electric tension (σc) under different Π. The σc dependent kp values were fitted with a theoretical equation, and the corresponding membrane tension (σoseq) at swelling equilibrium under Π was estimated. The estimated membrane tension agreed well with the theoretical calculation within the experimental error. Interestingly, the values of σoseq were almost same for both types of synthesized GUVs under same osmotic pressure. We also examined the sucrose leakage, due to large osmotic pressure-induced pore formation, from the inside of DOPG/DOPC/chol(46/39/15)-GUVs. The estimated membrane tension due to large Π at which sucrose leaked out was very similar to the electric tension at which GUVs were ruptured without Π. We explained the σc and Π induced pore formation in the lipid membranes of GUVs.Malay Kumar SarkarMohammad Abu Sayem KaralMarzuk AhmedMd Kabir AhamedShareef AhammedSabrina SharminSayed Ul Alam ShiblyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251690 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Malay Kumar Sarkar Mohammad Abu Sayem Karal Marzuk Ahmed Md Kabir Ahamed Shareef Ahammed Sabrina Sharmin Sayed Ul Alam Shibly Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.
description	Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique under different osmotic pressures (Π), and estimated the membrane tension due to Π. Two categories of GUVs were used in this study. One was prepared with a mixture of dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylcholine (DOPC) and cholesterol (chol) for obtaining more biological relevance while other with a mixture of DOPG and DOPC, with specific molar ratios. We determined the rate constant (kp) of rupture of DOPG/DOPC/chol (46/39/15)-GUVs and DOPG/DOPC (40/60)-GUVs induced by constant electric tension (σc) under different Π. The σc dependent kp values were fitted with a theoretical equation, and the corresponding membrane tension (σoseq) at swelling equilibrium under Π was estimated. The estimated membrane tension agreed well with the theoretical calculation within the experimental error. Interestingly, the values of σoseq were almost same for both types of synthesized GUVs under same osmotic pressure. We also examined the sucrose leakage, due to large osmotic pressure-induced pore formation, from the inside of DOPG/DOPC/chol(46/39/15)-GUVs. The estimated membrane tension due to large Π at which sucrose leaked out was very similar to the electric tension at which GUVs were ruptured without Π. We explained the σc and Π induced pore formation in the lipid membranes of GUVs.
format	article
author	Malay Kumar Sarkar Mohammad Abu Sayem Karal Marzuk Ahmed Md Kabir Ahamed Shareef Ahammed Sabrina Sharmin Sayed Ul Alam Shibly
author_facet	Malay Kumar Sarkar Mohammad Abu Sayem Karal Marzuk Ahmed Md Kabir Ahamed Shareef Ahammed Sabrina Sharmin Sayed Ul Alam Shibly
author_sort	Malay Kumar Sarkar
title	Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.
title_short	Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.
title_full	Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.
title_fullStr	Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.
title_full_unstemmed	Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.
title_sort	effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/eb8f88e3f7d04d3ea6507fb9b41af263
work_keys_str_mv	AT malaykumarsarkar effectsofosmoticpressureontheirreversibleelectroporationingiantlipidvesicles AT mohammadabusayemkaral effectsofosmoticpressureontheirreversibleelectroporationingiantlipidvesicles AT marzukahmed effectsofosmoticpressureontheirreversibleelectroporationingiantlipidvesicles AT mdkabirahamed effectsofosmoticpressureontheirreversibleelectroporationingiantlipidvesicles AT shareefahammed effectsofosmoticpressureontheirreversibleelectroporationingiantlipidvesicles AT sabrinasharmin effectsofosmoticpressureontheirreversibleelectroporationingiantlipidvesicles AT sayedulalamshibly effectsofosmoticpressureontheirreversibleelectroporationingiantlipidvesicles
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Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.

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