In silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids

Mevalonate pyrophosphate decarboxylase (MPD) is a key enzyme in terpenoid biosynthesis. MPD plays an important role in the upstream regulation of secondary plant metabolism. However, studies on the MPD gene are relatively very few despite its importance in plant metabolism. Currently, no systematic...

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Autores principales:	Cui Minghui, Lin Limei, Guo Hongyu, Zhang Duoduo, Zhang Jie, Cheng Wenwen, Song Xin, Xing Zhaobin, Long Yuehong
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	campanulids apiales mevalonate pyrophosphate decarboxylase gene family adaptive gene evolution Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/eb93fea9a1da44b8a32900fdb4e410f8
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spelling	oai:doaj.org-article:eb93fea9a1da44b8a32900fdb4e410f82021-12-05T14:10:41ZIn silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids2391-541210.1515/biol-2021-0103https://doaj.org/article/eb93fea9a1da44b8a32900fdb4e410f82021-09-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0103https://doaj.org/toc/2391-5412Mevalonate pyrophosphate decarboxylase (MPD) is a key enzyme in terpenoid biosynthesis. MPD plays an important role in the upstream regulation of secondary plant metabolism. However, studies on the MPD gene are relatively very few despite its importance in plant metabolism. Currently, no systematic analysis has been conducted on the MPD gene in plants under the order Apiales, which comprises important medicinal plants such as Panax ginseng and Panax notoginseng. This study sought to explore the structural characteristics of the MPD gene and the effect of adaptive evolution on the gene by comparing and analyzing MPD gene sequences of different campanulids species. For that, phylogenetic and adaptive evolution analyses were carried out using sequences for 11 Campanulids species. MPD sequence characteristics of each species were then analyzed, and the collinearity analysis of the genes was performed. As a result, a total of 21 MPD proteins were identified in 11 Campanulids species through BLAST analysis. Phylogenetic analysis, physical and chemical properties prediction, gene family analysis, and gene structure prediction showed that the MPD gene has undergone purifying selection and exhibited highly conserved structure. Analysis of physicochemical properties further showed that the MPD protein was a hydrophilic protein without a transmembrane region. Moreover, collinearity analysis in Apiales showed that MPD gene on chromosome 2 of D. carota and chromosome 1 of C. sativum were collinear. The findings showed that MPD gene is highly conserved. This may be a common characteristic of all essential enzymes in the biosynthesis pathways of medicinal plants. Notably, MPD gene is significantly affected by environmental factors which subsequently modulate its expression. The current study’s findings provide a basis for follow-up studies on MPD gene and key enzymes in other medicinal plants.Cui MinghuiLin LimeiGuo HongyuZhang DuoduoZhang JieCheng WenwenSong XinXing ZhaobinLong YuehongDe Gruyterarticlecampanulidsapialesmevalonate pyrophosphate decarboxylasegene familyadaptive gene evolutionBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 1022-1036 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	campanulids apiales mevalonate pyrophosphate decarboxylase gene family adaptive gene evolution Biology (General) QH301-705.5
spellingShingle	campanulids apiales mevalonate pyrophosphate decarboxylase gene family adaptive gene evolution Biology (General) QH301-705.5 Cui Minghui Lin Limei Guo Hongyu Zhang Duoduo Zhang Jie Cheng Wenwen Song Xin Xing Zhaobin Long Yuehong In silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids
description	Mevalonate pyrophosphate decarboxylase (MPD) is a key enzyme in terpenoid biosynthesis. MPD plays an important role in the upstream regulation of secondary plant metabolism. However, studies on the MPD gene are relatively very few despite its importance in plant metabolism. Currently, no systematic analysis has been conducted on the MPD gene in plants under the order Apiales, which comprises important medicinal plants such as Panax ginseng and Panax notoginseng. This study sought to explore the structural characteristics of the MPD gene and the effect of adaptive evolution on the gene by comparing and analyzing MPD gene sequences of different campanulids species. For that, phylogenetic and adaptive evolution analyses were carried out using sequences for 11 Campanulids species. MPD sequence characteristics of each species were then analyzed, and the collinearity analysis of the genes was performed. As a result, a total of 21 MPD proteins were identified in 11 Campanulids species through BLAST analysis. Phylogenetic analysis, physical and chemical properties prediction, gene family analysis, and gene structure prediction showed that the MPD gene has undergone purifying selection and exhibited highly conserved structure. Analysis of physicochemical properties further showed that the MPD protein was a hydrophilic protein without a transmembrane region. Moreover, collinearity analysis in Apiales showed that MPD gene on chromosome 2 of D. carota and chromosome 1 of C. sativum were collinear. The findings showed that MPD gene is highly conserved. This may be a common characteristic of all essential enzymes in the biosynthesis pathways of medicinal plants. Notably, MPD gene is significantly affected by environmental factors which subsequently modulate its expression. The current study’s findings provide a basis for follow-up studies on MPD gene and key enzymes in other medicinal plants.
format	article
author	Cui Minghui Lin Limei Guo Hongyu Zhang Duoduo Zhang Jie Cheng Wenwen Song Xin Xing Zhaobin Long Yuehong
author_facet	Cui Minghui Lin Limei Guo Hongyu Zhang Duoduo Zhang Jie Cheng Wenwen Song Xin Xing Zhaobin Long Yuehong
author_sort	Cui Minghui
title	In silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids
title_short	In silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids
title_full	In silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids
title_fullStr	In silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids
title_full_unstemmed	In silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids
title_sort	in silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in campanulids
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/eb93fea9a1da44b8a32900fdb4e410f8
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In silico/computational analysis of mevalonate pyrophosphate decarboxylase gene families in Campanulids

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