Optimized PCR conditions and increased shRNA fold representation improve reproducibility of pooled shRNA screens.

Optimized PCR conditions and increased shRNA fold representation improve reproducibility of pooled shRNA screens.

RNAi screening using pooled shRNA libraries is a valuable tool for identifying genetic regulators of biological processes. However, for a successful pooled shRNA screen, it is imperative to thoroughly optimize experimental conditions to obtain reproducible data. Here we performed viability screens w...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Žaklina Strezoska, Abel Licon, Josh Haimes, Katie Jansen Spayd, Kruti M Patel, Kevin Sullivan, Katarzyna Jastrzebski, Kaylene J Simpson, Devin Leake, Anja van Brabant Smith, Annaleen Vermeulen
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/eb94d0add74e441f93937a750fe12195
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares

Ejemplares similares