A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation

Abstract Cap-independent translation initiation plays crucial roles in fine-tuning gene expression under global translation shutdown conditions. Translation of uncapped or de-capped transcripts can be stimulated by Cap-independent translation enhancer (CITE) elements, but the mechanisms of CITE-medi...

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Autores principales:	Brandon M. Trainor, Arnab Ghosh, Dimitri G. Pestov, Christopher U. T. Hellen, Natalia Shcherbik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eb979a51c5a94d25b5b2eaade80f1f01
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spelling	oai:doaj.org-article:eb979a51c5a94d25b5b2eaade80f1f012021-12-02T10:47:54ZA translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation10.1038/s41598-021-82025-62045-2322https://doaj.org/article/eb979a51c5a94d25b5b2eaade80f1f012021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82025-6https://doaj.org/toc/2045-2322Abstract Cap-independent translation initiation plays crucial roles in fine-tuning gene expression under global translation shutdown conditions. Translation of uncapped or de-capped transcripts can be stimulated by Cap-independent translation enhancer (CITE) elements, but the mechanisms of CITE-mediated translation initiation remain understudied. Here, we characterized a short 5ʹ-UTR RNA sequence from black beetle virus, BBV-seq. Mutational analysis indicates that the entire BBV-seq is required for efficient translation initiation, but this sequence does not operate as an IRES-type module. In yeast cell-free translation extracts, BBV-seq promoted efficient initiation on cap-free mRNA using a scanning mechanism. Moreover, BBV-seq can increase translation efficiency resulting from conventional cap-dependent translation initiation. Using genetic approaches, we found that BBV-seq exploits RNA-binding properties of eIF4G1 to promote initiation. Thus, BBV-seq constitutes a previously uncharacterized short, linear CITE that influences eIF4G1 to initiate 5′ end-dependent, cap-independent translation. These findings bring new insights into CITE-mediated translational control of gene expression.Brandon M. TrainorArnab GhoshDimitri G. PestovChristopher U. T. HellenNatalia ShcherbikNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brandon M. Trainor Arnab Ghosh Dimitri G. Pestov Christopher U. T. Hellen Natalia Shcherbik A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation
description	Abstract Cap-independent translation initiation plays crucial roles in fine-tuning gene expression under global translation shutdown conditions. Translation of uncapped or de-capped transcripts can be stimulated by Cap-independent translation enhancer (CITE) elements, but the mechanisms of CITE-mediated translation initiation remain understudied. Here, we characterized a short 5ʹ-UTR RNA sequence from black beetle virus, BBV-seq. Mutational analysis indicates that the entire BBV-seq is required for efficient translation initiation, but this sequence does not operate as an IRES-type module. In yeast cell-free translation extracts, BBV-seq promoted efficient initiation on cap-free mRNA using a scanning mechanism. Moreover, BBV-seq can increase translation efficiency resulting from conventional cap-dependent translation initiation. Using genetic approaches, we found that BBV-seq exploits RNA-binding properties of eIF4G1 to promote initiation. Thus, BBV-seq constitutes a previously uncharacterized short, linear CITE that influences eIF4G1 to initiate 5′ end-dependent, cap-independent translation. These findings bring new insights into CITE-mediated translational control of gene expression.
format	article
author	Brandon M. Trainor Arnab Ghosh Dimitri G. Pestov Christopher U. T. Hellen Natalia Shcherbik
author_facet	Brandon M. Trainor Arnab Ghosh Dimitri G. Pestov Christopher U. T. Hellen Natalia Shcherbik
author_sort	Brandon M. Trainor
title	A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation
title_short	A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation
title_full	A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation
title_fullStr	A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation
title_full_unstemmed	A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation
title_sort	translation enhancer element from black beetle virus engages yeast eif4g1 to drive cap-independent translation initiation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eb979a51c5a94d25b5b2eaade80f1f01
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A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation

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