CHANGES OF INNATE IMMUNITY INDEXES IN SEVERE ASTHMA IN CHILDREN

At the present time, the role of innate immunity in pathogenesis of bronchial asthma (BA) is actively studied, in particular, significance of TLRs and cytokines. The study included 42 patients with severe bronchial asthma (from 3 to 12 years old), and 67 healthy children at the same age. Expression...

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Autores principales:	L. V. Gankovskaya, L. S. Namazova-Baranova, G. V. Poriadin, V. V. Grechenko, V. A. Gankovsky, A. A. Alekseeva, Zh. M. Salmashi, A. N. Kazimirsky, B. G. Bragvadze, O. A. Svitich
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	tlr2 tlr4 tlr9 proinflammatory cytokine expression asthma Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/eb9e06dda0eb44dbb2f8961f9f64d941
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spelling	oai:doaj.org-article:eb9e06dda0eb44dbb2f8961f9f64d9412021-11-18T08:03:48ZCHANGES OF INNATE IMMUNITY INDEXES IN SEVERE ASTHMA IN CHILDREN1563-06252313-741X10.15789/1563-0625-2019-1-99-106https://doaj.org/article/eb9e06dda0eb44dbb2f8961f9f64d9412019-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1703https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAt the present time, the role of innate immunity in pathogenesis of bronchial asthma (BA) is actively studied, in particular, significance of TLRs and cytokines. The study included 42 patients with severe bronchial asthma (from 3 to 12 years old), and 67 healthy children at the same age. Expression of TLR2, TLR4, and TLR9 genes was evaluated by PCR-RT from the scrapings of nasal mucosa; cytokines (IL-33, TSLP, IL-4, TGF-β1 and IL-28B) were assayed in nasal swabs by ELISA technique. The main results were as follows: an increased gene expression of TLR2, TLR4, TLR9 genes was revealed in the nasal mucosa scraps from the patients with bronchial asthma as compared to healthy children. We have also measured the contents of important cytokines secreted by the respiratory epithelium in the course of TLRs activation. The study of IL-33, TSLP, IL-4 in nasal samples revealed significantly increased concentrations of these cytokines in the patients with severe BA against the control group. A study of TGF-βlevels in nasal cavity swabs revealed a significant decrease of this regulatory cytokine in the group of pediatric patients with asthma. Worth of note, evaluation of antiviral IL-28B cytokine in the group of patients with severe BA showed a significant downward trend, in comparison to the control indexes. Hence, one may conclude on some disturbances of local innate immunity system in the patients with severe BA which manifest as hyperexpression of TLRs genes, increased production of proinflammatory and epithelial cytokines, decreased production of antiviral IL-28B cytokine, and TGF-β1.L. V. GankovskayaL. S. Namazova-BaranovaG. V. PoriadinV. V. GrechenkoV. A. GankovskyA. A. AlekseevaZh. M. SalmashiA. N. KazimirskyB. G. BragvadzeO. A. SvitichSPb RAACIarticletlr2tlr4tlr9proinflammatory cytokineexpressionasthmaImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 1, Pp 99-106 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	tlr2 tlr4 tlr9 proinflammatory cytokine expression asthma Immunologic diseases. Allergy RC581-607
spellingShingle	tlr2 tlr4 tlr9 proinflammatory cytokine expression asthma Immunologic diseases. Allergy RC581-607 L. V. Gankovskaya L. S. Namazova-Baranova G. V. Poriadin V. V. Grechenko V. A. Gankovsky A. A. Alekseeva Zh. M. Salmashi A. N. Kazimirsky B. G. Bragvadze O. A. Svitich CHANGES OF INNATE IMMUNITY INDEXES IN SEVERE ASTHMA IN CHILDREN
description	At the present time, the role of innate immunity in pathogenesis of bronchial asthma (BA) is actively studied, in particular, significance of TLRs and cytokines. The study included 42 patients with severe bronchial asthma (from 3 to 12 years old), and 67 healthy children at the same age. Expression of TLR2, TLR4, and TLR9 genes was evaluated by PCR-RT from the scrapings of nasal mucosa; cytokines (IL-33, TSLP, IL-4, TGF-β1 and IL-28B) were assayed in nasal swabs by ELISA technique. The main results were as follows: an increased gene expression of TLR2, TLR4, TLR9 genes was revealed in the nasal mucosa scraps from the patients with bronchial asthma as compared to healthy children. We have also measured the contents of important cytokines secreted by the respiratory epithelium in the course of TLRs activation. The study of IL-33, TSLP, IL-4 in nasal samples revealed significantly increased concentrations of these cytokines in the patients with severe BA against the control group. A study of TGF-βlevels in nasal cavity swabs revealed a significant decrease of this regulatory cytokine in the group of pediatric patients with asthma. Worth of note, evaluation of antiviral IL-28B cytokine in the group of patients with severe BA showed a significant downward trend, in comparison to the control indexes. Hence, one may conclude on some disturbances of local innate immunity system in the patients with severe BA which manifest as hyperexpression of TLRs genes, increased production of proinflammatory and epithelial cytokines, decreased production of antiviral IL-28B cytokine, and TGF-β1.
format	article
author	L. V. Gankovskaya L. S. Namazova-Baranova G. V. Poriadin V. V. Grechenko V. A. Gankovsky A. A. Alekseeva Zh. M. Salmashi A. N. Kazimirsky B. G. Bragvadze O. A. Svitich
author_facet	L. V. Gankovskaya L. S. Namazova-Baranova G. V. Poriadin V. V. Grechenko V. A. Gankovsky A. A. Alekseeva Zh. M. Salmashi A. N. Kazimirsky B. G. Bragvadze O. A. Svitich
author_sort	L. V. Gankovskaya
title	CHANGES OF INNATE IMMUNITY INDEXES IN SEVERE ASTHMA IN CHILDREN
title_short	CHANGES OF INNATE IMMUNITY INDEXES IN SEVERE ASTHMA IN CHILDREN
title_full	CHANGES OF INNATE IMMUNITY INDEXES IN SEVERE ASTHMA IN CHILDREN
title_fullStr	CHANGES OF INNATE IMMUNITY INDEXES IN SEVERE ASTHMA IN CHILDREN
title_full_unstemmed	CHANGES OF INNATE IMMUNITY INDEXES IN SEVERE ASTHMA IN CHILDREN
title_sort	changes of innate immunity indexes in severe asthma in children
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/eb9e06dda0eb44dbb2f8961f9f64d941
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CHANGES OF INNATE IMMUNITY INDEXES IN SEVERE ASTHMA IN CHILDREN

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