Augmented TLR2 expression on monocytes in both human Kawasaki disease and a mouse model of coronary arteritis.

<h4>Background</h4>Kawasaki disease (KD) of unknown immunopathogenesis is an acute febrile systemic vasculitis and the leading cause of acquired heart diseases in childhood. To search for a better strategy for the prevention and treatment of KD, this study compared and validated human KD...

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Autores principales: I-Chun Lin, Ho-Chang Kuo, Ying-Jui Lin, Feng-Shen Wang, Lin Wang, Shun-Chen Huang, Shao-Ju Chien, Chien-Fu Huang, Chih-Lu Wang, Hong-Ren Yu, Rong-Fu Chen, Kuender D Yang
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Publicado: Public Library of Science (PLoS) 2012
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spelling oai:doaj.org-article:eba314ddc95e4eab82d121b0c5b1cae22021-11-18T07:14:46ZAugmented TLR2 expression on monocytes in both human Kawasaki disease and a mouse model of coronary arteritis.1932-620310.1371/journal.pone.0038635https://doaj.org/article/eba314ddc95e4eab82d121b0c5b1cae22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22737215/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Kawasaki disease (KD) of unknown immunopathogenesis is an acute febrile systemic vasculitis and the leading cause of acquired heart diseases in childhood. To search for a better strategy for the prevention and treatment of KD, this study compared and validated human KD immunopathogenesis in a mouse model of Lactobacillus casei cell wall extract (LCWE)-induced coronary arteritis.<h4>Methods</h4>Recruited subjects fulfilled the criteria of KD and were admitted for intravenous gamma globulin (IVIG) treatment at the Kaohsiung Chang Gung Memorial Hospital from 2001 to 2009. Blood samples from KD patients were collected before and after IVIG treatment, and cardiovascular abnormalities were examined by transthoracic echocardiography. Wild-type male BALB/c mice (4-week-old) were intraperitoneally injected with LCWE (1 mg/mL) to induce coronary arteritis. The induced immune response in mice was examined on days 1, 3, 7, and 14 post injections, and histopathology studies were performed on days 7 and 14.<h4>Results</h4>Both human KD patients and LCWE-treated mice developed coronary arteritis, myocarditis, valvulitis, and pericarditis, as well as elevated plasma levels of interleukin (IL)-2, IL-6, IL-10, monocyte chemoattractant protein (MCP)-1, and tumor necrosis factor (TNF)-α in acute phase. Most of these proinflammatory cytokines declined to normal levels in mice, whereas normal levels were achieved in patients only after IVIG treatment, with a few exceptions. Toll-like receptor (TLR)-2, but not TLR4 surface enhancement on circulating CD14+ monocytes, was augmented in KD patients before IVIG treatment and in LCWE-treated mice, which declined in patients after IVIG treatment.<h4>Conclusion</h4>This result suggests that that not only TLR2 augmentation on CD14+ monocytes might be an inflammatory marker for both human KD patients and LCWE-induced CAL mouse model but also this model is feasible for studying therapeutic strategies of coronary arteritis in human KD by modulating TLR2-mediated immune activation on CD14+ monocytes.I-Chun LinHo-Chang KuoYing-Jui LinFeng-Shen WangLin WangShun-Chen HuangShao-Ju ChienChien-Fu HuangChih-Lu WangHong-Ren YuRong-Fu ChenKuender D YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e38635 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
I-Chun Lin
Ho-Chang Kuo
Ying-Jui Lin
Feng-Shen Wang
Lin Wang
Shun-Chen Huang
Shao-Ju Chien
Chien-Fu Huang
Chih-Lu Wang
Hong-Ren Yu
Rong-Fu Chen
Kuender D Yang
Augmented TLR2 expression on monocytes in both human Kawasaki disease and a mouse model of coronary arteritis.
description <h4>Background</h4>Kawasaki disease (KD) of unknown immunopathogenesis is an acute febrile systemic vasculitis and the leading cause of acquired heart diseases in childhood. To search for a better strategy for the prevention and treatment of KD, this study compared and validated human KD immunopathogenesis in a mouse model of Lactobacillus casei cell wall extract (LCWE)-induced coronary arteritis.<h4>Methods</h4>Recruited subjects fulfilled the criteria of KD and were admitted for intravenous gamma globulin (IVIG) treatment at the Kaohsiung Chang Gung Memorial Hospital from 2001 to 2009. Blood samples from KD patients were collected before and after IVIG treatment, and cardiovascular abnormalities were examined by transthoracic echocardiography. Wild-type male BALB/c mice (4-week-old) were intraperitoneally injected with LCWE (1 mg/mL) to induce coronary arteritis. The induced immune response in mice was examined on days 1, 3, 7, and 14 post injections, and histopathology studies were performed on days 7 and 14.<h4>Results</h4>Both human KD patients and LCWE-treated mice developed coronary arteritis, myocarditis, valvulitis, and pericarditis, as well as elevated plasma levels of interleukin (IL)-2, IL-6, IL-10, monocyte chemoattractant protein (MCP)-1, and tumor necrosis factor (TNF)-α in acute phase. Most of these proinflammatory cytokines declined to normal levels in mice, whereas normal levels were achieved in patients only after IVIG treatment, with a few exceptions. Toll-like receptor (TLR)-2, but not TLR4 surface enhancement on circulating CD14+ monocytes, was augmented in KD patients before IVIG treatment and in LCWE-treated mice, which declined in patients after IVIG treatment.<h4>Conclusion</h4>This result suggests that that not only TLR2 augmentation on CD14+ monocytes might be an inflammatory marker for both human KD patients and LCWE-induced CAL mouse model but also this model is feasible for studying therapeutic strategies of coronary arteritis in human KD by modulating TLR2-mediated immune activation on CD14+ monocytes.
format article
author I-Chun Lin
Ho-Chang Kuo
Ying-Jui Lin
Feng-Shen Wang
Lin Wang
Shun-Chen Huang
Shao-Ju Chien
Chien-Fu Huang
Chih-Lu Wang
Hong-Ren Yu
Rong-Fu Chen
Kuender D Yang
author_facet I-Chun Lin
Ho-Chang Kuo
Ying-Jui Lin
Feng-Shen Wang
Lin Wang
Shun-Chen Huang
Shao-Ju Chien
Chien-Fu Huang
Chih-Lu Wang
Hong-Ren Yu
Rong-Fu Chen
Kuender D Yang
author_sort I-Chun Lin
title Augmented TLR2 expression on monocytes in both human Kawasaki disease and a mouse model of coronary arteritis.
title_short Augmented TLR2 expression on monocytes in both human Kawasaki disease and a mouse model of coronary arteritis.
title_full Augmented TLR2 expression on monocytes in both human Kawasaki disease and a mouse model of coronary arteritis.
title_fullStr Augmented TLR2 expression on monocytes in both human Kawasaki disease and a mouse model of coronary arteritis.
title_full_unstemmed Augmented TLR2 expression on monocytes in both human Kawasaki disease and a mouse model of coronary arteritis.
title_sort augmented tlr2 expression on monocytes in both human kawasaki disease and a mouse model of coronary arteritis.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/eba314ddc95e4eab82d121b0c5b1cae2
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