Sevoflurane attenuates pulmonary inflammation and ventilator-induced lung injury by upregulation of HO-1 mRNA expression in mice

Sevoflurane attenuates pulmonary inflammation and ventilator-induced lung injury by upregulation of HO-1 mRNA expression in mice

Xiang-qing Xiong, Li-na Lin, Liang-rong Wang, Li-da JinDepartment of Anesthesiology, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou, People's Republic of ChinaBackground: Mechanical ventilation has been documented to paradoxically cause lung injury. As a commonly used...

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Autores principales: Xiong XQ, Lin LN, Wang LR, Jin LD
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2013
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Medicine (General)
R5-920
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spelling oai:doaj.org-article:eba753bc856347d2941cb1bff44e6ddc2021-12-02T05:02:10ZSevoflurane attenuates pulmonary inflammation and ventilator-induced lung injury by upregulation of HO-1 mRNA expression in mice1176-91141178-2013https://doaj.org/article/eba753bc856347d2941cb1bff44e6ddc2013-03-01T00:00:00Zhttp://www.dovepress.com/sevoflurane-attenuates-pulmonary-inflammation-and-ventilator-induced-l-a12454https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Xiang-qing Xiong, Li-na Lin, Liang-rong Wang, Li-da JinDepartment of Anesthesiology, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou, People's Republic of ChinaBackground: Mechanical ventilation has been documented to paradoxically cause lung injury. As a commonly used volatile anesthetic, sevoflurane has been proven to possess antiinflammatory and antioxidative properties. This study aims to investigate the protective effects of sevoflurane on inflammation and ventilator-induced lung injury during mechanical ventilation in healthy mice.Methods: The adult healthy mice were divided into four groups, each consisting of ten subjects: mice in group Con-LVT and group Sev-LVT were ventilated with tidal volumes of 8 mL/kg for 4 hours, while those in group Con-HVT and group Sev-HVT were ventilated with tidal volumes of 16 mL/kg instead. Control mice (group Con-LVT and Con-HVT) were subjected to fresh air, while sevoflurane-treated mice (groups Sev-LVT and Sev-HVT) were subjected to air mixed with 1 vol% sevoflurane. After 4 hours of ventilation, the bronchoalveolar lavage (BAL) fluid was collected and analyzed for the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10. Lung homogenates were harvested to detect the expression of nuclear factor-kppa B (NF-кB) and heme oxygenase (HO)-1 mRNA by reverse transcription-polymerase chain reaction method. Lung damage was evaluated using the modified Ventilator-Induced Lung Injury histological scoring system.Results: Compared to group Con-LVT, the levels of TNF-α, IL-1β, IL-6, and IL-10 in BAL fluid, mRNA expressions of NF-кB and HO-1 in lung tissue, and lung injury scores were significantly increased in group Con-HVT; compared to group Con-HVT, group Sev-HVT BAL samples showed decreased levels of TNF-α, IL-1β, and IL-6; they also showed increased levels of IL-10, the downregulation of NF-кB mRNA, and HO-1 mRNA upregulation; the lung injury scores were significantly lower in group Sev-HVT than group Con-HVT.Conclusion: Mechanical ventilation with high tidal volume might lead to lung injury, which could be significantly, but not completely, attenuated by sevoflurane inhalation by inhibiting the NF-кB-mediated proinflammatory cytokine generation and upregulating HO-1 expression.Keywords: mechanical ventilation, sevoflurane, inflammation, lung injuryXiong XQLin LNWang LRJin LDDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1075-1081 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Xiong XQ
Lin LN
Wang LR
Jin LD
Sevoflurane attenuates pulmonary inflammation and ventilator-induced lung injury by upregulation of HO-1 mRNA expression in mice
description Xiang-qing Xiong, Li-na Lin, Liang-rong Wang, Li-da JinDepartment of Anesthesiology, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou, People's Republic of ChinaBackground: Mechanical ventilation has been documented to paradoxically cause lung injury. As a commonly used volatile anesthetic, sevoflurane has been proven to possess antiinflammatory and antioxidative properties. This study aims to investigate the protective effects of sevoflurane on inflammation and ventilator-induced lung injury during mechanical ventilation in healthy mice.Methods: The adult healthy mice were divided into four groups, each consisting of ten subjects: mice in group Con-LVT and group Sev-LVT were ventilated with tidal volumes of 8 mL/kg for 4 hours, while those in group Con-HVT and group Sev-HVT were ventilated with tidal volumes of 16 mL/kg instead. Control mice (group Con-LVT and Con-HVT) were subjected to fresh air, while sevoflurane-treated mice (groups Sev-LVT and Sev-HVT) were subjected to air mixed with 1 vol% sevoflurane. After 4 hours of ventilation, the bronchoalveolar lavage (BAL) fluid was collected and analyzed for the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10. Lung homogenates were harvested to detect the expression of nuclear factor-kppa B (NF-кB) and heme oxygenase (HO)-1 mRNA by reverse transcription-polymerase chain reaction method. Lung damage was evaluated using the modified Ventilator-Induced Lung Injury histological scoring system.Results: Compared to group Con-LVT, the levels of TNF-α, IL-1β, IL-6, and IL-10 in BAL fluid, mRNA expressions of NF-кB and HO-1 in lung tissue, and lung injury scores were significantly increased in group Con-HVT; compared to group Con-HVT, group Sev-HVT BAL samples showed decreased levels of TNF-α, IL-1β, and IL-6; they also showed increased levels of IL-10, the downregulation of NF-кB mRNA, and HO-1 mRNA upregulation; the lung injury scores were significantly lower in group Sev-HVT than group Con-HVT.Conclusion: Mechanical ventilation with high tidal volume might lead to lung injury, which could be significantly, but not completely, attenuated by sevoflurane inhalation by inhibiting the NF-кB-mediated proinflammatory cytokine generation and upregulating HO-1 expression.Keywords: mechanical ventilation, sevoflurane, inflammation, lung injury
format article
author Xiong XQ
Lin LN
Wang LR
Jin LD
author_facet Xiong XQ
Lin LN
Wang LR
Jin LD
author_sort Xiong XQ
title Sevoflurane attenuates pulmonary inflammation and ventilator-induced lung injury by upregulation of HO-1 mRNA expression in mice
title_short Sevoflurane attenuates pulmonary inflammation and ventilator-induced lung injury by upregulation of HO-1 mRNA expression in mice
title_full Sevoflurane attenuates pulmonary inflammation and ventilator-induced lung injury by upregulation of HO-1 mRNA expression in mice
title_fullStr Sevoflurane attenuates pulmonary inflammation and ventilator-induced lung injury by upregulation of HO-1 mRNA expression in mice
title_full_unstemmed Sevoflurane attenuates pulmonary inflammation and ventilator-induced lung injury by upregulation of HO-1 mRNA expression in mice
title_sort sevoflurane attenuates pulmonary inflammation and ventilator-induced lung injury by upregulation of ho-1 mrna expression in mice
publisher Dove Medical Press
publishDate 2013
url https://doaj.org/article/eba753bc856347d2941cb1bff44e6ddc
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AT wanglr sevofluraneattenuatespulmonaryinflammationandventilatorinducedlunginjurybyupregulationofho1mrnaexpressioninmice
AT jinld sevofluraneattenuatespulmonaryinflammationandventilatorinducedlunginjurybyupregulationofho1mrnaexpressioninmice
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