UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates.

The risk of sepsis through bacterial transmission is one of the most serious problems in platelet transfusion. In processing platelet concentrates (PCs), several methods have been put into practice to minimize the risk of bacterial transmission, such as stringent monitoring by cultivation assays and...

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Autores principales:	Tomoya Hayashi, Kumiko Oguma, Yoshihiro Fujimura, Rika A Furuta, Mitsunobu Tanaka, Mikako Masaki, Yasuhito Shinbata, Takafumi Kimura, Yoshihiko Tani, Fumiya Hirayama, Yoshihiro Takihara, Koki Takahashi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eba895aea34f4e7c97672990c03a0dc9
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spelling	oai:doaj.org-article:eba895aea34f4e7c97672990c03a0dc92021-11-25T06:19:05ZUV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates.1932-620310.1371/journal.pone.0251650https://doaj.org/article/eba895aea34f4e7c97672990c03a0dc92021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251650https://doaj.org/toc/1932-6203The risk of sepsis through bacterial transmission is one of the most serious problems in platelet transfusion. In processing platelet concentrates (PCs), several methods have been put into practice to minimize the risk of bacterial transmission, such as stringent monitoring by cultivation assays and inactivation treatment by photoirradiation with or without chemical agents. As another potential option, we applied a light-emitting diode (LED) with a peak emission wavelength of 265 nm, which has been shown to be effective for water, to disinfect PCs. In a bench-scale UV-LED exposure setup, a 10-min irradiation, corresponding to an average fluence of 9.2 mJ/cm2, resulted in >2.0 log, 1.0 log, and 0.6 log inactivation (mean, n = 6) of Escherichia coli, Staphylococcus aureus, and Bacillus cereus, respectively, in non-diluted plasma PCs. After a 30-min exposure, platelet counts decreased slightly (18 ± 7%: mean ± SD, n = 7); however, platelet surface expressions of CD42b, CD61, CD62P, and PAC-1 binding did not change significantly (P>0.005), and agonist-induced aggregation and adhesion/aggregation under flow conditions were well maintained. Our findings indicated that the 265 nm UV-LED has high potential as a novel disinfection method to ensure the microbial safety of platelet transfusion.Tomoya HayashiKumiko OgumaYoshihiro FujimuraRika A FurutaMitsunobu TanakaMikako MasakiYasuhito ShinbataTakafumi KimuraYoshihiko TaniFumiya HirayamaYoshihiro TakiharaKoki TakahashiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251650 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomoya Hayashi Kumiko Oguma Yoshihiro Fujimura Rika A Furuta Mitsunobu Tanaka Mikako Masaki Yasuhito Shinbata Takafumi Kimura Yoshihiko Tani Fumiya Hirayama Yoshihiro Takihara Koki Takahashi UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates.
description	The risk of sepsis through bacterial transmission is one of the most serious problems in platelet transfusion. In processing platelet concentrates (PCs), several methods have been put into practice to minimize the risk of bacterial transmission, such as stringent monitoring by cultivation assays and inactivation treatment by photoirradiation with or without chemical agents. As another potential option, we applied a light-emitting diode (LED) with a peak emission wavelength of 265 nm, which has been shown to be effective for water, to disinfect PCs. In a bench-scale UV-LED exposure setup, a 10-min irradiation, corresponding to an average fluence of 9.2 mJ/cm2, resulted in >2.0 log, 1.0 log, and 0.6 log inactivation (mean, n = 6) of Escherichia coli, Staphylococcus aureus, and Bacillus cereus, respectively, in non-diluted plasma PCs. After a 30-min exposure, platelet counts decreased slightly (18 ± 7%: mean ± SD, n = 7); however, platelet surface expressions of CD42b, CD61, CD62P, and PAC-1 binding did not change significantly (P>0.005), and agonist-induced aggregation and adhesion/aggregation under flow conditions were well maintained. Our findings indicated that the 265 nm UV-LED has high potential as a novel disinfection method to ensure the microbial safety of platelet transfusion.
format	article
author	Tomoya Hayashi Kumiko Oguma Yoshihiro Fujimura Rika A Furuta Mitsunobu Tanaka Mikako Masaki Yasuhito Shinbata Takafumi Kimura Yoshihiko Tani Fumiya Hirayama Yoshihiro Takihara Koki Takahashi
author_facet	Tomoya Hayashi Kumiko Oguma Yoshihiro Fujimura Rika A Furuta Mitsunobu Tanaka Mikako Masaki Yasuhito Shinbata Takafumi Kimura Yoshihiko Tani Fumiya Hirayama Yoshihiro Takihara Koki Takahashi
author_sort	Tomoya Hayashi
title	UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates.
title_short	UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates.
title_full	UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates.
title_fullStr	UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates.
title_full_unstemmed	UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates.
title_sort	uv light-emitting diode (uv-led) at 265 nm as a potential light source for disinfecting human platelet concentrates.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/eba895aea34f4e7c97672990c03a0dc9
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UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates.

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