Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

V&aacute;clav Vanecek,1,5 Vitalii Zablotskiim,2 Serhiy Forostyakm,1,5 Jir&iacute; Ružickam,1 V&iacute;t Herynekm,3 Michal Babic,4 Pavla Jendelov&aacute;,1,5 &Scaron;&aacute;rka Kubinov&aacute;,1 Alexandr Dejneka,2 Eva Sykov&aacute;1,51I...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vaněček V, Zablotskii V, Forostyak S, Růžička J, Herynek V, Babič M, Jendelov&aacute; P, Kubinov&aacute; &Scaron;, Dejneka A, Sykov&aacute; E
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ebac36c3f68f4c0bb5ac6ad76e8f0239
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ebac36c3f68f4c0bb5ac6ad76e8f0239
record_format	dspace
spelling	oai:doaj.org-article:ebac36c3f68f4c0bb5ac6ad76e8f02392021-12-02T01:08:09ZHighly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury1176-91141178-2013https://doaj.org/article/ebac36c3f68f4c0bb5ac6ad76e8f02392012-07-01T00:00:00Zhttp://www.dovepress.com/highly-efficient-magnetic-targeting-of-mesenchymal-stem-cells-in-spina-a10413https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013V&aacute;clav Vanecek,1,5 Vitalii Zablotskiim,2 Serhiy Forostyakm,1,5 Jir&iacute; Ružickam,1 V&iacute;t Herynekm,3 Michal Babic,4 Pavla Jendelov&aacute;,1,5 &Scaron;&aacute;rka Kubinov&aacute;,1 Alexandr Dejneka,2 Eva Sykov&aacute;1,51Institute of Experimental Medicine AS CR, Prague, Czech Republic; 2Institute of Physics AS CR, Prague, Czech Republic; 3MR-Unit, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; 4Institute of Macromolecular Chemistry AS CR, Prague, Czech Republic; 5Department of Neuroscience, 2nd Faculty of Medicine, Charles University, Prague, Czech RepublicAbstract: The transplantation of mesenchymal stem cells (MSC) is currently under study as a therapeutic approach for spinal cord injury, and the number of transplanted cells that reach the lesioned tissue is one of the critical parameters. In this study, intrathecally transplanted cells labeled with superparamagnetic iron oxide nanoparticles were guided by a magnetic field and successfully targeted near the lesion site in the rat spinal cord. Magnetic resonance imaging and histological analysis revealed significant differences in cell numbers and cell distribution near the lesion site under the magnet in comparison to control groups. The cell distribution correlated well with the calculated distribution of magnetic forces exerted on the transplanted cells in the subarachnoid space and lesion site. The kinetics of the cells&rsquo; accumulation near the lesion site is described within the framework of a mathematical model that reveals those parameters critical for cell targeting and suggests ways to enhance the efficiency of magnetic cell delivery. In particular, we show that the targeting efficiency can be increased by using magnets that produce spatially modulated stray fields. Such magnetic systems with tunable geometric parameters may provide the additional level of control needed to enhance the efficiency of stem cell delivery in spinal cord injury.Keywords: magnetism, mesenchymal stem cell, nanoparticle, spinal cord injury, modelingVaněček VZablotskii VForostyak SRůžička JHerynek VBabič MJendelov&aacute; PKubinov&aacute; &Scaron;Dejneka ASykov&aacute; EDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3719-3730 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Vaněček V Zablotskii V Forostyak S Růžička J Herynek V Babič M Jendelov&aacute; P Kubinov&aacute; &Scaron; Dejneka A Sykov&aacute; E Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury
description	V&aacute;clav Vanecek,1,5 Vitalii Zablotskiim,2 Serhiy Forostyakm,1,5 Jir&iacute; Ružickam,1 V&iacute;t Herynekm,3 Michal Babic,4 Pavla Jendelov&aacute;,1,5 &Scaron;&aacute;rka Kubinov&aacute;,1 Alexandr Dejneka,2 Eva Sykov&aacute;1,51Institute of Experimental Medicine AS CR, Prague, Czech Republic; 2Institute of Physics AS CR, Prague, Czech Republic; 3MR-Unit, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; 4Institute of Macromolecular Chemistry AS CR, Prague, Czech Republic; 5Department of Neuroscience, 2nd Faculty of Medicine, Charles University, Prague, Czech RepublicAbstract: The transplantation of mesenchymal stem cells (MSC) is currently under study as a therapeutic approach for spinal cord injury, and the number of transplanted cells that reach the lesioned tissue is one of the critical parameters. In this study, intrathecally transplanted cells labeled with superparamagnetic iron oxide nanoparticles were guided by a magnetic field and successfully targeted near the lesion site in the rat spinal cord. Magnetic resonance imaging and histological analysis revealed significant differences in cell numbers and cell distribution near the lesion site under the magnet in comparison to control groups. The cell distribution correlated well with the calculated distribution of magnetic forces exerted on the transplanted cells in the subarachnoid space and lesion site. The kinetics of the cells&rsquo; accumulation near the lesion site is described within the framework of a mathematical model that reveals those parameters critical for cell targeting and suggests ways to enhance the efficiency of magnetic cell delivery. In particular, we show that the targeting efficiency can be increased by using magnets that produce spatially modulated stray fields. Such magnetic systems with tunable geometric parameters may provide the additional level of control needed to enhance the efficiency of stem cell delivery in spinal cord injury.Keywords: magnetism, mesenchymal stem cell, nanoparticle, spinal cord injury, modeling
format	article
author	Vaněček V Zablotskii V Forostyak S Růžička J Herynek V Babič M Jendelov&aacute; P Kubinov&aacute; &Scaron; Dejneka A Sykov&aacute; E
author_facet	Vaněček V Zablotskii V Forostyak S Růžička J Herynek V Babič M Jendelov&aacute; P Kubinov&aacute; &Scaron; Dejneka A Sykov&aacute; E
author_sort	Vaněček V
title	Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury
title_short	Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury
title_full	Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury
title_fullStr	Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury
title_full_unstemmed	Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury
title_sort	highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/ebac36c3f68f4c0bb5ac6ad76e8f0239
work_keys_str_mv	AT vanecekv highlyefficientmagnetictargetingofmesenchymalstemcellsinspinalcordinjury AT zablotskiiv highlyefficientmagnetictargetingofmesenchymalstemcellsinspinalcordinjury AT forostyaks highlyefficientmagnetictargetingofmesenchymalstemcellsinspinalcordinjury AT ruzickaj highlyefficientmagnetictargetingofmesenchymalstemcellsinspinalcordinjury AT herynekv highlyefficientmagnetictargetingofmesenchymalstemcellsinspinalcordinjury AT babicm highlyefficientmagnetictargetingofmesenchymalstemcellsinspinalcordinjury AT jendelovampaacutep highlyefficientmagnetictargetingofmesenchymalstemcellsinspinalcordinjury AT kubinovampaacuteampscaron highlyefficientmagnetictargetingofmesenchymalstemcellsinspinalcordinjury AT dejnekaa highlyefficientmagnetictargetingofmesenchymalstemcellsinspinalcordinjury AT sykovampaacutee highlyefficientmagnetictargetingofmesenchymalstemcellsinspinalcordinjury
_version_	1718403289965395968

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Ejemplares similares